Expression of Toll-Like Receptors 2, 4 and Nuclear Factor Kappa B in Mucosal Lesions of Human Otitis: Pattern and Relationship in a Clinical Immunohistochemical Study
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Аутори
Ješić, SnežanaJotić, Ana
Tomanović, Nada
Živković, Maja
Kolaković, Ana
Stanković, Aleksandra
Чланак у часопису
Метаподаци
Приказ свих података о документуАпстракт
Objectives: The objectives were to detect and compare the expression of toll-like receptors (TLRs) 2, 4 and nuclear factor kappa B in mucosal lesions of chronic otitis. Methods: Fifty-five tissue samples obtained from children and adults operated on for otitis were investigated by semiquantitative immunohistochemical methods using polyclonal antibodies for TLR 2, 4 and NFkB. Kruskal-Wallis, Mann-Whitney, and Kendalls tau rank correlation tests were used. Results: Stronger expression of TLR2, 4 was found in inflamed mucosa than in the control for children and adults (TLR2: H = 23.86, P LT .001; TLR4: H = 22.80, P LT .001) (TLR2: H = 17.53, P LT .001; TLR4: H = 11.99, P LT .001); in cholesteatoma perimatrix compared to tubotympanic lesions in children (TLR2: H = 11.06, P = .004; TLR4: H = 10.61, P = .005) and adults (TLR2: H = 10.73, P =.013; TLR4: H = 9.65, P = .021). No differences were found in NFkB expression (H = 0.042, P = .99). Significant correlations were found for all pairs of ...molecules in cholesteatoma and tubotympanic mucosa of adults (TLR2, 4: P = .002, P LT .001; TLR2-NfkB: P = .032, P = .021; TLR4-NFkB: P =.035, P = .0013), only TLR4-NFkB in tubotympanic otitis of children (P = .026). Conclusions: Toll-like receptors 2, 4 and NFkB mediate inflammation in cholesteatoma and mucosal lesions of tubotympanic otitis in children and adults. Significant correlations between all pairs of molecules in all samples were detected in adults, but only TLR4-NFkB in children.
Кључне речи:
toll-like receptors / nuclear factor kappa B / otitis / humansИзвор:
Annals of Otology Rhinology and Laryngology, 2014, 123, 6, 434-441Финансирање / пројекти:
- Генетска основа хуманих васкуларних и инфламаторних болести (RS-MESTD-Basic Research (BR or ON)-175085)
DOI: 10.1177/0003489414527229
ISSN: 0003-4894; 1943-572X
PubMed: 24690988
WoS: 000337974100010
Scopus: 2-s2.0-84903549085
Колекције
Институција/група
VinčaTY - JOUR AU - Ješić, Snežana AU - Jotić, Ana AU - Tomanović, Nada AU - Živković, Maja AU - Kolaković, Ana AU - Stanković, Aleksandra PY - 2014 UR - https://vinar.vin.bg.ac.rs/handle/123456789/6051 AB - Objectives: The objectives were to detect and compare the expression of toll-like receptors (TLRs) 2, 4 and nuclear factor kappa B in mucosal lesions of chronic otitis. Methods: Fifty-five tissue samples obtained from children and adults operated on for otitis were investigated by semiquantitative immunohistochemical methods using polyclonal antibodies for TLR 2, 4 and NFkB. Kruskal-Wallis, Mann-Whitney, and Kendalls tau rank correlation tests were used. Results: Stronger expression of TLR2, 4 was found in inflamed mucosa than in the control for children and adults (TLR2: H = 23.86, P LT .001; TLR4: H = 22.80, P LT .001) (TLR2: H = 17.53, P LT .001; TLR4: H = 11.99, P LT .001); in cholesteatoma perimatrix compared to tubotympanic lesions in children (TLR2: H = 11.06, P = .004; TLR4: H = 10.61, P = .005) and adults (TLR2: H = 10.73, P =.013; TLR4: H = 9.65, P = .021). No differences were found in NFkB expression (H = 0.042, P = .99). Significant correlations were found for all pairs of molecules in cholesteatoma and tubotympanic mucosa of adults (TLR2, 4: P = .002, P LT .001; TLR2-NfkB: P = .032, P = .021; TLR4-NFkB: P =.035, P = .0013), only TLR4-NFkB in tubotympanic otitis of children (P = .026). Conclusions: Toll-like receptors 2, 4 and NFkB mediate inflammation in cholesteatoma and mucosal lesions of tubotympanic otitis in children and adults. Significant correlations between all pairs of molecules in all samples were detected in adults, but only TLR4-NFkB in children. T2 - Annals of Otology Rhinology and Laryngology T1 - Expression of Toll-Like Receptors 2, 4 and Nuclear Factor Kappa B in Mucosal Lesions of Human Otitis: Pattern and Relationship in a Clinical Immunohistochemical Study VL - 123 IS - 6 SP - 434 EP - 441 DO - 10.1177/0003489414527229 ER -
@article{ author = "Ješić, Snežana and Jotić, Ana and Tomanović, Nada and Živković, Maja and Kolaković, Ana and Stanković, Aleksandra", year = "2014", abstract = "Objectives: The objectives were to detect and compare the expression of toll-like receptors (TLRs) 2, 4 and nuclear factor kappa B in mucosal lesions of chronic otitis. Methods: Fifty-five tissue samples obtained from children and adults operated on for otitis were investigated by semiquantitative immunohistochemical methods using polyclonal antibodies for TLR 2, 4 and NFkB. Kruskal-Wallis, Mann-Whitney, and Kendalls tau rank correlation tests were used. Results: Stronger expression of TLR2, 4 was found in inflamed mucosa than in the control for children and adults (TLR2: H = 23.86, P LT .001; TLR4: H = 22.80, P LT .001) (TLR2: H = 17.53, P LT .001; TLR4: H = 11.99, P LT .001); in cholesteatoma perimatrix compared to tubotympanic lesions in children (TLR2: H = 11.06, P = .004; TLR4: H = 10.61, P = .005) and adults (TLR2: H = 10.73, P =.013; TLR4: H = 9.65, P = .021). No differences were found in NFkB expression (H = 0.042, P = .99). Significant correlations were found for all pairs of molecules in cholesteatoma and tubotympanic mucosa of adults (TLR2, 4: P = .002, P LT .001; TLR2-NfkB: P = .032, P = .021; TLR4-NFkB: P =.035, P = .0013), only TLR4-NFkB in tubotympanic otitis of children (P = .026). Conclusions: Toll-like receptors 2, 4 and NFkB mediate inflammation in cholesteatoma and mucosal lesions of tubotympanic otitis in children and adults. Significant correlations between all pairs of molecules in all samples were detected in adults, but only TLR4-NFkB in children.", journal = "Annals of Otology Rhinology and Laryngology", title = "Expression of Toll-Like Receptors 2, 4 and Nuclear Factor Kappa B in Mucosal Lesions of Human Otitis: Pattern and Relationship in a Clinical Immunohistochemical Study", volume = "123", number = "6", pages = "434-441", doi = "10.1177/0003489414527229" }
Ješić, S., Jotić, A., Tomanović, N., Živković, M., Kolaković, A.,& Stanković, A.. (2014). Expression of Toll-Like Receptors 2, 4 and Nuclear Factor Kappa B in Mucosal Lesions of Human Otitis: Pattern and Relationship in a Clinical Immunohistochemical Study. in Annals of Otology Rhinology and Laryngology, 123(6), 434-441. https://doi.org/10.1177/0003489414527229
Ješić S, Jotić A, Tomanović N, Živković M, Kolaković A, Stanković A. Expression of Toll-Like Receptors 2, 4 and Nuclear Factor Kappa B in Mucosal Lesions of Human Otitis: Pattern and Relationship in a Clinical Immunohistochemical Study. in Annals of Otology Rhinology and Laryngology. 2014;123(6):434-441. doi:10.1177/0003489414527229 .
Ješić, Snežana, Jotić, Ana, Tomanović, Nada, Živković, Maja, Kolaković, Ana, Stanković, Aleksandra, "Expression of Toll-Like Receptors 2, 4 and Nuclear Factor Kappa B in Mucosal Lesions of Human Otitis: Pattern and Relationship in a Clinical Immunohistochemical Study" in Annals of Otology Rhinology and Laryngology, 123, no. 6 (2014):434-441, https://doi.org/10.1177/0003489414527229 . .