Apolipoprotein E gene polymorphisms as risk factors for carotid atherosclerosis
Apstrakt
Background/Aim. Atherosclerosis is still the leading cause of death in Western world. Development of atherosclerotic plaque involves accumulation of inflammatory cells, lipids, smooth muscle cells and extracellular matrix proteins in the intima of the vascular wall. Apolipoprotein E participates in the transport of exogenous cholesterol, endogenously synthesized lipids and triglycerides in the organism. Apolipoprotein E gene has been identified as one of the candidate genes for atherosclerosis. Previous studies in different populations have clearly implicated apolipoprotein E genetic variation (epsilon polymorphisms) as a major modulator of low density lipoprotein cholesterol levels. Data considering apolipoprotein E polymorphisrns in relation to carotid atherosclerosis gave results that are not in full compliance. The aim of present study was to investigate the apolipoprotein E polymorphisms in association with carotid plaque presence, apolipoprotein E and lipid serum levels in patien...ts with carotid atherosclerosis from Serbia. Methods. The study group enrolled 495 participants: 285 controls and 210 consecutive patients with carotid atherosclerosis who underwent carotid endarterectomy. Genotyping of apolipoprotein E polymorphisms were done using polymerase chain reaction and restriction fragment length polymorphism methods. Results. Patients had significantly decreased frequency of the epsilon 2 allele compared to controls. Patients who carry at least one epsilon 2 allele had a significantly higher level of serum apolipoprotein E and significantly lower low density lipoprotein cholesterol levels compared to those who do not carry this allele. Conclusion. Our results suggest protective effect of apolipoprotein E epsilon 2 allele on susceptibility for carotid plaque presence as well as low density lipoprotein cholesterol lowering effect in Serbian patients with carotid atherosclerosis. Further research of multiple gene and environmental factors that contribute to the appearance and the progression of atherosclerosis should be continued with respect to different populations.
Ključne reči:
genetic predisposition to disease / carotid artery disease / polymorphism / genetic / apolipoproteins eIzvor:
Vojnosanitetski pregled, 2014, 71, 4, 362-367Finansiranje / projekti:
- Genetska osnova humanih vaskularnih i inflamatornih bolesti (RS-MESTD-Basic Research (BR or ON)-175085)
- Integralna studija identifikacije regionalnih genetskih faktora rizika i faktora rizika životne sredine za masovne nezarazne bolesti humane populacije u Srbiji - INGEMA_S (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41028)
DOI: 10.2298/VSP1404362Z
ISSN: 0042-8450
PubMed: 24783416
WoS: 000334570800005
Scopus: 2-s2.0-84897932129
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Zurnic, Irena AU - Đurić, Tamara AU - Končar, Igor AU - Stanković, Aleksandra AU - Dinčić, Dragan AU - Živković, Maja PY - 2014 UR - https://vinar.vin.bg.ac.rs/handle/123456789/5961 AB - Background/Aim. Atherosclerosis is still the leading cause of death in Western world. Development of atherosclerotic plaque involves accumulation of inflammatory cells, lipids, smooth muscle cells and extracellular matrix proteins in the intima of the vascular wall. Apolipoprotein E participates in the transport of exogenous cholesterol, endogenously synthesized lipids and triglycerides in the organism. Apolipoprotein E gene has been identified as one of the candidate genes for atherosclerosis. Previous studies in different populations have clearly implicated apolipoprotein E genetic variation (epsilon polymorphisms) as a major modulator of low density lipoprotein cholesterol levels. Data considering apolipoprotein E polymorphisrns in relation to carotid atherosclerosis gave results that are not in full compliance. The aim of present study was to investigate the apolipoprotein E polymorphisms in association with carotid plaque presence, apolipoprotein E and lipid serum levels in patients with carotid atherosclerosis from Serbia. Methods. The study group enrolled 495 participants: 285 controls and 210 consecutive patients with carotid atherosclerosis who underwent carotid endarterectomy. Genotyping of apolipoprotein E polymorphisms were done using polymerase chain reaction and restriction fragment length polymorphism methods. Results. Patients had significantly decreased frequency of the epsilon 2 allele compared to controls. Patients who carry at least one epsilon 2 allele had a significantly higher level of serum apolipoprotein E and significantly lower low density lipoprotein cholesterol levels compared to those who do not carry this allele. Conclusion. Our results suggest protective effect of apolipoprotein E epsilon 2 allele on susceptibility for carotid plaque presence as well as low density lipoprotein cholesterol lowering effect in Serbian patients with carotid atherosclerosis. Further research of multiple gene and environmental factors that contribute to the appearance and the progression of atherosclerosis should be continued with respect to different populations. T2 - Vojnosanitetski pregled T1 - Apolipoprotein E gene polymorphisms as risk factors for carotid atherosclerosis VL - 71 IS - 4 SP - 362 EP - 367 DO - 10.2298/VSP1404362Z ER -
@article{ author = "Zurnic, Irena and Đurić, Tamara and Končar, Igor and Stanković, Aleksandra and Dinčić, Dragan and Živković, Maja", year = "2014", abstract = "Background/Aim. Atherosclerosis is still the leading cause of death in Western world. Development of atherosclerotic plaque involves accumulation of inflammatory cells, lipids, smooth muscle cells and extracellular matrix proteins in the intima of the vascular wall. Apolipoprotein E participates in the transport of exogenous cholesterol, endogenously synthesized lipids and triglycerides in the organism. Apolipoprotein E gene has been identified as one of the candidate genes for atherosclerosis. Previous studies in different populations have clearly implicated apolipoprotein E genetic variation (epsilon polymorphisms) as a major modulator of low density lipoprotein cholesterol levels. Data considering apolipoprotein E polymorphisrns in relation to carotid atherosclerosis gave results that are not in full compliance. The aim of present study was to investigate the apolipoprotein E polymorphisms in association with carotid plaque presence, apolipoprotein E and lipid serum levels in patients with carotid atherosclerosis from Serbia. Methods. The study group enrolled 495 participants: 285 controls and 210 consecutive patients with carotid atherosclerosis who underwent carotid endarterectomy. Genotyping of apolipoprotein E polymorphisms were done using polymerase chain reaction and restriction fragment length polymorphism methods. Results. Patients had significantly decreased frequency of the epsilon 2 allele compared to controls. Patients who carry at least one epsilon 2 allele had a significantly higher level of serum apolipoprotein E and significantly lower low density lipoprotein cholesterol levels compared to those who do not carry this allele. Conclusion. Our results suggest protective effect of apolipoprotein E epsilon 2 allele on susceptibility for carotid plaque presence as well as low density lipoprotein cholesterol lowering effect in Serbian patients with carotid atherosclerosis. Further research of multiple gene and environmental factors that contribute to the appearance and the progression of atherosclerosis should be continued with respect to different populations.", journal = "Vojnosanitetski pregled", title = "Apolipoprotein E gene polymorphisms as risk factors for carotid atherosclerosis", volume = "71", number = "4", pages = "362-367", doi = "10.2298/VSP1404362Z" }
Zurnic, I., Đurić, T., Končar, I., Stanković, A., Dinčić, D.,& Živković, M.. (2014). Apolipoprotein E gene polymorphisms as risk factors for carotid atherosclerosis. in Vojnosanitetski pregled, 71(4), 362-367. https://doi.org/10.2298/VSP1404362Z
Zurnic I, Đurić T, Končar I, Stanković A, Dinčić D, Živković M. Apolipoprotein E gene polymorphisms as risk factors for carotid atherosclerosis. in Vojnosanitetski pregled. 2014;71(4):362-367. doi:10.2298/VSP1404362Z .
Zurnic, Irena, Đurić, Tamara, Končar, Igor, Stanković, Aleksandra, Dinčić, Dragan, Živković, Maja, "Apolipoprotein E gene polymorphisms as risk factors for carotid atherosclerosis" in Vojnosanitetski pregled, 71, no. 4 (2014):362-367, https://doi.org/10.2298/VSP1404362Z . .