Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells
2014
Authors
Milićević, Zorka T.Bajić, Vladan P.
Živković, Lada
Kasapović, Jelena
Anđelković, Uros
Spremo-Potparević, Biljana
Article
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In breast carcinoma, disruption of the p53 pathway is one of the most common genetic alterations. The observation that the p53 can express multiple protein isoforms adds a novel level of complexity to the outcome of p53 mutations. p53 expression was analysed by Western immunoblotting and immunohistochemistry using monoclonal antibodies DO-7, Pab240, and polyclonal antiserum CM-1. The more frequently p53-positive nuclear staining has been found in the invasive breast tumors. One of the most intriguing findings is that mutant p53 appears as discrete dot-shaped regions within the nucleus of breast cancer cells. In many malignant cells, the nucleolar sequestration of p53 is evident. These observations support the view that the nucleolus is involved directly in the mediation of p53 function or indirectly by the control of the localization of p53 interplayers. p53 expressed in the nuclear fraction of breast cancer cells revealed a wide spectrum of isoforms. p53 isoforms Lambda Np53 (47 kDa) ...and Lambda 133p53 beta (35 kDa), known as dominant-negative repressors of p53 function, were detected as the most predominant variants in nuclei of invasive breast carcinoma cells. The isoforms expressed also varied between individual tumors, indicating potential roles of these p53 variants in human breast cancer.
Source:
Scientific World Journal, 2014Funding / projects:
- Cell Cycle Aberrations and the Impact of Oxidative Stress in Neurodegenerative Processes and Malignant Transformation of the Cell (RS-MESTD-Basic Research (BR or ON)-173034)
DOI: 10.1155/2014/618698
ISSN: 1537-744X
WoS: 000330388500001
Scopus: 2-s2.0-84893205816
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VinčaTY - JOUR AU - Milićević, Zorka T. AU - Bajić, Vladan P. AU - Živković, Lada AU - Kasapović, Jelena AU - Anđelković, Uros AU - Spremo-Potparević, Biljana PY - 2014 UR - https://vinar.vin.bg.ac.rs/handle/123456789/5855 AB - In breast carcinoma, disruption of the p53 pathway is one of the most common genetic alterations. The observation that the p53 can express multiple protein isoforms adds a novel level of complexity to the outcome of p53 mutations. p53 expression was analysed by Western immunoblotting and immunohistochemistry using monoclonal antibodies DO-7, Pab240, and polyclonal antiserum CM-1. The more frequently p53-positive nuclear staining has been found in the invasive breast tumors. One of the most intriguing findings is that mutant p53 appears as discrete dot-shaped regions within the nucleus of breast cancer cells. In many malignant cells, the nucleolar sequestration of p53 is evident. These observations support the view that the nucleolus is involved directly in the mediation of p53 function or indirectly by the control of the localization of p53 interplayers. p53 expressed in the nuclear fraction of breast cancer cells revealed a wide spectrum of isoforms. p53 isoforms Lambda Np53 (47 kDa) and Lambda 133p53 beta (35 kDa), known as dominant-negative repressors of p53 function, were detected as the most predominant variants in nuclei of invasive breast carcinoma cells. The isoforms expressed also varied between individual tumors, indicating potential roles of these p53 variants in human breast cancer. T2 - Scientific World Journal T1 - Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells DO - 10.1155/2014/618698 ER -
@article{ author = "Milićević, Zorka T. and Bajić, Vladan P. and Živković, Lada and Kasapović, Jelena and Anđelković, Uros and Spremo-Potparević, Biljana", year = "2014", abstract = "In breast carcinoma, disruption of the p53 pathway is one of the most common genetic alterations. The observation that the p53 can express multiple protein isoforms adds a novel level of complexity to the outcome of p53 mutations. p53 expression was analysed by Western immunoblotting and immunohistochemistry using monoclonal antibodies DO-7, Pab240, and polyclonal antiserum CM-1. The more frequently p53-positive nuclear staining has been found in the invasive breast tumors. One of the most intriguing findings is that mutant p53 appears as discrete dot-shaped regions within the nucleus of breast cancer cells. In many malignant cells, the nucleolar sequestration of p53 is evident. These observations support the view that the nucleolus is involved directly in the mediation of p53 function or indirectly by the control of the localization of p53 interplayers. p53 expressed in the nuclear fraction of breast cancer cells revealed a wide spectrum of isoforms. p53 isoforms Lambda Np53 (47 kDa) and Lambda 133p53 beta (35 kDa), known as dominant-negative repressors of p53 function, were detected as the most predominant variants in nuclei of invasive breast carcinoma cells. The isoforms expressed also varied between individual tumors, indicating potential roles of these p53 variants in human breast cancer.", journal = "Scientific World Journal", title = "Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells", doi = "10.1155/2014/618698" }
Milićević, Z. T., Bajić, V. P., Živković, L., Kasapović, J., Anđelković, U.,& Spremo-Potparević, B.. (2014). Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells. in Scientific World Journal. https://doi.org/10.1155/2014/618698
Milićević ZT, Bajić VP, Živković L, Kasapović J, Anđelković U, Spremo-Potparević B. Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells. in Scientific World Journal. 2014;. doi:10.1155/2014/618698 .
Milićević, Zorka T., Bajić, Vladan P., Živković, Lada, Kasapović, Jelena, Anđelković, Uros, Spremo-Potparević, Biljana, "Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells" in Scientific World Journal (2014), https://doi.org/10.1155/2014/618698 . .