Interactions of Platinum and Ruthenium Coordination Complexes with Pancreatic Phospholipase A(2) and Phospholipids Investigated by MALDI TOF Mass Spectrometry
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Phospholipase A(2) is involved in propagation of inflammatory processes and carcinogenesis through its role in phospholipid metabolism, and release of arachidonic acid and lysophospholipids. Recent findings on correlation between elevated PLA(2) activity and metastatic cancer render this enzyme an attractive target for cancer therapy. On the other hand, due to a broad range of oxidation states under physiological conditions and a high affinity for protein binding, platinum and ruthenium coordination complexes are promising candidates for PLA(2) inhibitors. In this article, we discuss the interactions of Pt and Ru coordination complexes with PLA(2) and phospholipids, as well as the application of MALDI-TOF mass spectrometry for screening PLA(2) inhibitors. Owing to the ability of this technique to simultaneously detect and monitor changes in substrate and product concentrations, the inhibitor mechanisms of both Pt and Ru complexes with various ligands were determined.
Keywords:Platinum complexes / Ruthenium complexes / Inhibitors / Phospholipids / Mass spectrometry / Phospholipase A
Source:Chemistry and Biodiversity, 2013, 10, 11, 1972-1986
- Ministry of Sciences and Technological Development of the Republic of Serbia , German Academic Exchange Service