The expression of ACE, ACE2, AT1 and AT2 receptors in the heart of fructose fed ovariectomized rats-Effect of estradiol
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Аутори
Bundalo, Maja M.Tepavčević, Snežana
Romić, Snježana Đ.
Korićanac, Goran
Živković, Maja
Stanković, Aleksandra
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Background: Heart disease or diabetes could be promoted in people who consume sugar. High dietary
intake of fructose also increases risk for heart disease. The effects of fructose diet on metabolism, lipid
profile and blood pressure are dependent of sex. Renin-angiotensin system (RAS) impairs insulin sensi-
tivity, whereas hyperinsulinemia and insulin resistance promotes the development of cardiovascular dis-
orders. We examined the effects of fructose rich diet (FRD) and estradiol (E2) on the expression of ACE,
ACE2, AT1R and AT2R in the rat heart.
Methods and Results: 21 day old female rats were divided in control group and FRD group. At two
weeks before sacrifice, animals were ovariectomised and half of the FRD rats were subjected to E2 re-
placement. FRD increased ACE (p<0.001) and AT1R (p<0.05) protein expression, while AT2R expression
was decreased (p<0.05). ACE2 protein expression was unaltered. mRNA expression for AT1R was unal-
tered, as assessed by qPCR. E2 treatmen...t significantly decreased protein expression for ACE (p<0.001)
and AT1R (p<0.01), while ACE2 (p<0.01) and AT2R (p<0.01) expression was increased. Effect of E2 treat-
ment on AT1R gene expression was not statistically significant but showed trend toward lowering AT1R
gene expression. We didn’t detected AT2R gene expression.
Conclusion: FRD affects almost all components of the RAS in the rat heart.The increase of the ACE and
AT1R protein expression and decrease of AT2R expression could be the the way that leads to the devel-
opment of cardiovascular disorders. The E2 shows strong protective effect in FRD through reversion of the
changes in RAS components expression.
Извор:
Cardiology, 2013, 126, Suppl. 2, 353-353Колекције
Институција/група
VinčaTY - CONF AU - Bundalo, Maja M. AU - Tepavčević, Snežana AU - Romić, Snježana Đ. AU - Korićanac, Goran AU - Živković, Maja AU - Stanković, Aleksandra PY - 2013 UR - https://vinar.vin.bg.ac.rs/handle/123456789/5741 AB - Background: Heart disease or diabetes could be promoted in people who consume sugar. High dietary intake of fructose also increases risk for heart disease. The effects of fructose diet on metabolism, lipid profile and blood pressure are dependent of sex. Renin-angiotensin system (RAS) impairs insulin sensi- tivity, whereas hyperinsulinemia and insulin resistance promotes the development of cardiovascular dis- orders. We examined the effects of fructose rich diet (FRD) and estradiol (E2) on the expression of ACE, ACE2, AT1R and AT2R in the rat heart. Methods and Results: 21 day old female rats were divided in control group and FRD group. At two weeks before sacrifice, animals were ovariectomised and half of the FRD rats were subjected to E2 re- placement. FRD increased ACE (p<0.001) and AT1R (p<0.05) protein expression, while AT2R expression was decreased (p<0.05). ACE2 protein expression was unaltered. mRNA expression for AT1R was unal- tered, as assessed by qPCR. E2 treatment significantly decreased protein expression for ACE (p<0.001) and AT1R (p<0.01), while ACE2 (p<0.01) and AT2R (p<0.01) expression was increased. Effect of E2 treat- ment on AT1R gene expression was not statistically significant but showed trend toward lowering AT1R gene expression. We didn’t detected AT2R gene expression. Conclusion: FRD affects almost all components of the RAS in the rat heart.The increase of the ACE and AT1R protein expression and decrease of AT2R expression could be the the way that leads to the devel- opment of cardiovascular disorders. The E2 shows strong protective effect in FRD through reversion of the changes in RAS components expression. C3 - Cardiology T1 - The expression of ACE, ACE2, AT1 and AT2 receptors in the heart of fructose fed ovariectomized rats-Effect of estradiol VL - 126 IS - Suppl. 2 SP - 353 EP - 353 DO - 10.1159/000355890 UR - https://hdl.handle.net/21.15107/rcub_vinar_5741 ER -
@conference{ author = "Bundalo, Maja M. and Tepavčević, Snežana and Romić, Snježana Đ. and Korićanac, Goran and Živković, Maja and Stanković, Aleksandra", year = "2013", abstract = "Background: Heart disease or diabetes could be promoted in people who consume sugar. High dietary intake of fructose also increases risk for heart disease. The effects of fructose diet on metabolism, lipid profile and blood pressure are dependent of sex. Renin-angiotensin system (RAS) impairs insulin sensi- tivity, whereas hyperinsulinemia and insulin resistance promotes the development of cardiovascular dis- orders. We examined the effects of fructose rich diet (FRD) and estradiol (E2) on the expression of ACE, ACE2, AT1R and AT2R in the rat heart. Methods and Results: 21 day old female rats were divided in control group and FRD group. At two weeks before sacrifice, animals were ovariectomised and half of the FRD rats were subjected to E2 re- placement. FRD increased ACE (p<0.001) and AT1R (p<0.05) protein expression, while AT2R expression was decreased (p<0.05). ACE2 protein expression was unaltered. mRNA expression for AT1R was unal- tered, as assessed by qPCR. E2 treatment significantly decreased protein expression for ACE (p<0.001) and AT1R (p<0.01), while ACE2 (p<0.01) and AT2R (p<0.01) expression was increased. Effect of E2 treat- ment on AT1R gene expression was not statistically significant but showed trend toward lowering AT1R gene expression. We didn’t detected AT2R gene expression. Conclusion: FRD affects almost all components of the RAS in the rat heart.The increase of the ACE and AT1R protein expression and decrease of AT2R expression could be the the way that leads to the devel- opment of cardiovascular disorders. The E2 shows strong protective effect in FRD through reversion of the changes in RAS components expression.", journal = "Cardiology", title = "The expression of ACE, ACE2, AT1 and AT2 receptors in the heart of fructose fed ovariectomized rats-Effect of estradiol", volume = "126", number = "Suppl. 2", pages = "353-353", doi = "10.1159/000355890", url = "https://hdl.handle.net/21.15107/rcub_vinar_5741" }
Bundalo, M. M., Tepavčević, S., Romić, S. Đ., Korićanac, G., Živković, M.,& Stanković, A.. (2013). The expression of ACE, ACE2, AT1 and AT2 receptors in the heart of fructose fed ovariectomized rats-Effect of estradiol. in Cardiology, 126(Suppl. 2), 353-353. https://doi.org/10.1159/000355890 https://hdl.handle.net/21.15107/rcub_vinar_5741
Bundalo MM, Tepavčević S, Romić SĐ, Korićanac G, Živković M, Stanković A. The expression of ACE, ACE2, AT1 and AT2 receptors in the heart of fructose fed ovariectomized rats-Effect of estradiol. in Cardiology. 2013;126(Suppl. 2):353-353. doi:10.1159/000355890 https://hdl.handle.net/21.15107/rcub_vinar_5741 .
Bundalo, Maja M., Tepavčević, Snežana, Romić, Snježana Đ., Korićanac, Goran, Živković, Maja, Stanković, Aleksandra, "The expression of ACE, ACE2, AT1 and AT2 receptors in the heart of fructose fed ovariectomized rats-Effect of estradiol" in Cardiology, 126, no. Suppl. 2 (2013):353-353, https://doi.org/10.1159/000355890 ., https://hdl.handle.net/21.15107/rcub_vinar_5741 .