Atomic Force Microscopy Characterization of the External Cortical Bone Surface in Young and Elderly Women: Potential Nanostructural Traces of Periosteal Bone Apposition During Aging
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Autori
Milovanović, PetarĐurić, Marija
Nešković, Olivera M.
Djonic, Danijela
Potočnik, Jelena
Nikolic, Slobodan
Stoiljković, Milovan
Živković, Vladimir
Rakočević, Zlatko Lj.
Članak u časopisu
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
On the basis of the suggestion that bone nanostructure bears tissue age information and may reflect surface deposition/modification processes, we performed nanoscale characterization of the external cortical bone surface at the femoral neck in women using atomic force microscopy (AFM). The specific aims were to assess age-related differences in bone nanostructure and explore the existence of nanostructural traces of potential bone apposition at this surface. Our findings revealed that the external cortical surface represents a continuous phase composed of densely packed mineral grains. Although the grains varied in size and shape, there was a domination of small grains indicative of freshly deposited bone (mean grain size: young, 35 nm; old, 37 nm; p. 0.05). Advanced quantitative analysis of surface morphological patterns revealed comparable roughness and complexity of the surface, suggesting a similar rate of mineral particle deposition at the surface in both groups. Calcium/phosphoru...s ratio, a measure of bone tissue age, was within the same range in both groups. In summary, our AFM analyses showed consistent nanostructural and compositional bone features, suggesting existence of new bone at the periosteal bone surface in both young and elderly women. Considering observed age-related increase in the neck diameter, AFM findings may support the theory of continuous bone apposition at the periosteal surface.
Ključne reči:
atomic force microscopy (AFM) / bone / nanostructure / periosteal surface / aging / femoral neckIzvor:
Microscopy and Microanalysis, 2013, 19, 5, 1341-1349Finansiranje / projekti:
- Funkcionalni, funkcionalizovani i usavršeni nano materijali (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45005)
DOI: 10.1017/S1431927613001761
ISSN: 1431-9276
PubMed: 23764147
WoS: 000324550900029
Scopus: 2-s2.0-84884546987
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Milovanović, Petar AU - Đurić, Marija AU - Nešković, Olivera M. AU - Djonic, Danijela AU - Potočnik, Jelena AU - Nikolic, Slobodan AU - Stoiljković, Milovan AU - Živković, Vladimir AU - Rakočević, Zlatko Lj. PY - 2013 UR - https://vinar.vin.bg.ac.rs/handle/123456789/5687 AB - On the basis of the suggestion that bone nanostructure bears tissue age information and may reflect surface deposition/modification processes, we performed nanoscale characterization of the external cortical bone surface at the femoral neck in women using atomic force microscopy (AFM). The specific aims were to assess age-related differences in bone nanostructure and explore the existence of nanostructural traces of potential bone apposition at this surface. Our findings revealed that the external cortical surface represents a continuous phase composed of densely packed mineral grains. Although the grains varied in size and shape, there was a domination of small grains indicative of freshly deposited bone (mean grain size: young, 35 nm; old, 37 nm; p. 0.05). Advanced quantitative analysis of surface morphological patterns revealed comparable roughness and complexity of the surface, suggesting a similar rate of mineral particle deposition at the surface in both groups. Calcium/phosphorus ratio, a measure of bone tissue age, was within the same range in both groups. In summary, our AFM analyses showed consistent nanostructural and compositional bone features, suggesting existence of new bone at the periosteal bone surface in both young and elderly women. Considering observed age-related increase in the neck diameter, AFM findings may support the theory of continuous bone apposition at the periosteal surface. T2 - Microscopy and Microanalysis T1 - Atomic Force Microscopy Characterization of the External Cortical Bone Surface in Young and Elderly Women: Potential Nanostructural Traces of Periosteal Bone Apposition During Aging VL - 19 IS - 5 SP - 1341 EP - 1349 DO - 10.1017/S1431927613001761 ER -
@article{ author = "Milovanović, Petar and Đurić, Marija and Nešković, Olivera M. and Djonic, Danijela and Potočnik, Jelena and Nikolic, Slobodan and Stoiljković, Milovan and Živković, Vladimir and Rakočević, Zlatko Lj.", year = "2013", abstract = "On the basis of the suggestion that bone nanostructure bears tissue age information and may reflect surface deposition/modification processes, we performed nanoscale characterization of the external cortical bone surface at the femoral neck in women using atomic force microscopy (AFM). The specific aims were to assess age-related differences in bone nanostructure and explore the existence of nanostructural traces of potential bone apposition at this surface. Our findings revealed that the external cortical surface represents a continuous phase composed of densely packed mineral grains. Although the grains varied in size and shape, there was a domination of small grains indicative of freshly deposited bone (mean grain size: young, 35 nm; old, 37 nm; p. 0.05). Advanced quantitative analysis of surface morphological patterns revealed comparable roughness and complexity of the surface, suggesting a similar rate of mineral particle deposition at the surface in both groups. Calcium/phosphorus ratio, a measure of bone tissue age, was within the same range in both groups. In summary, our AFM analyses showed consistent nanostructural and compositional bone features, suggesting existence of new bone at the periosteal bone surface in both young and elderly women. Considering observed age-related increase in the neck diameter, AFM findings may support the theory of continuous bone apposition at the periosteal surface.", journal = "Microscopy and Microanalysis", title = "Atomic Force Microscopy Characterization of the External Cortical Bone Surface in Young and Elderly Women: Potential Nanostructural Traces of Periosteal Bone Apposition During Aging", volume = "19", number = "5", pages = "1341-1349", doi = "10.1017/S1431927613001761" }
Milovanović, P., Đurić, M., Nešković, O. M., Djonic, D., Potočnik, J., Nikolic, S., Stoiljković, M., Živković, V.,& Rakočević, Z. Lj.. (2013). Atomic Force Microscopy Characterization of the External Cortical Bone Surface in Young and Elderly Women: Potential Nanostructural Traces of Periosteal Bone Apposition During Aging. in Microscopy and Microanalysis, 19(5), 1341-1349. https://doi.org/10.1017/S1431927613001761
Milovanović P, Đurić M, Nešković OM, Djonic D, Potočnik J, Nikolic S, Stoiljković M, Živković V, Rakočević ZL. Atomic Force Microscopy Characterization of the External Cortical Bone Surface in Young and Elderly Women: Potential Nanostructural Traces of Periosteal Bone Apposition During Aging. in Microscopy and Microanalysis. 2013;19(5):1341-1349. doi:10.1017/S1431927613001761 .
Milovanović, Petar, Đurić, Marija, Nešković, Olivera M., Djonic, Danijela, Potočnik, Jelena, Nikolic, Slobodan, Stoiljković, Milovan, Živković, Vladimir, Rakočević, Zlatko Lj., "Atomic Force Microscopy Characterization of the External Cortical Bone Surface in Young and Elderly Women: Potential Nanostructural Traces of Periosteal Bone Apposition During Aging" in Microscopy and Microanalysis, 19, no. 5 (2013):1341-1349, https://doi.org/10.1017/S1431927613001761 . .