Single and Combined Effects of Acute and Chronic Non-Thermal Stressors on Rat Interscapular Brown Adipose Tissue Metabolic Activity
2013
Аутори
Cvijic, GordanaLakić, Iva
Vujovic, R.
Jasnic, N.
Djurasevic, S.
Dronjak, Slađana
Đorđević, Jelena D.
Чланак у часопису
Метаподаци
Приказ свих података о документуАпстракт
The aim of this study was to examine whether the thermogenic potential of rat interscapular brown adipose tissue (IBAT) changes in response to acute and/or chronic exposure to non-thermal stressors (immobilization and isolation), by measuring the uncoupling protein 1 (UCP-1) content, MAO-A, SOD and CAT activities, as well as the number of IBAT sympathetic noradrenaline-containing nerve fibers. Both acute immobilization (2 h) and chronic isolation (21 days), as well as their combined effects, significantly increased the IBAT UCP-1 content in comparison to non-stressed animals. When applied individually, stressors increased the number of sympathetic fibers in comparison to controls, whereas in combination they decreased it. The activity of IBAT monoamine oxidase-A (MAO-A) decreased under the influence of each stressor independent of its type or duration. SOD activity coincided with MAO-A decrement, whereas CAT activity had an opposite pattern of changes. We conclude that acute and chroni...c exposure to non-thermal stressors, immobilization and isolation, respectively, affect the metabolic potential of rat IBAT, judging by the increase in UCP-1 content and sympathetic outflow. However, when acute immobilization was applied as a novel stressor to previously chronically isolated animals, an increase in the UCP-1 content was accompanied by a lower IBAT sympathetic outflow, suggesting that IBAT metabolic function under various stress condition is not solely dependent on SNS activity.
Кључне речи:
Isolation / immobilization / IBAT / UCP-1 / MAO-A / SOD / catalaseИзвор:
Archives of Biological Sciences, 2013, 65, 3, 919-927Финансирање / пројекти:
- Ефекат метаболичких и неметаболичких стресора на експресију и деловање неуроендокриних регулатора енергетске хомеостазе (RS-MESTD-Basic Research (BR or ON)-173023)
DOI: 10.2298/ABS1303919C
ISSN: 0354-4664
WoS: 000322348800014
Scopus: 2-s2.0-84882616747
Колекције
Институција/група
VinčaTY - JOUR AU - Cvijic, Gordana AU - Lakić, Iva AU - Vujovic, R. AU - Jasnic, N. AU - Djurasevic, S. AU - Dronjak, Slađana AU - Đorđević, Jelena D. PY - 2013 UR - https://vinar.vin.bg.ac.rs/handle/123456789/5603 AB - The aim of this study was to examine whether the thermogenic potential of rat interscapular brown adipose tissue (IBAT) changes in response to acute and/or chronic exposure to non-thermal stressors (immobilization and isolation), by measuring the uncoupling protein 1 (UCP-1) content, MAO-A, SOD and CAT activities, as well as the number of IBAT sympathetic noradrenaline-containing nerve fibers. Both acute immobilization (2 h) and chronic isolation (21 days), as well as their combined effects, significantly increased the IBAT UCP-1 content in comparison to non-stressed animals. When applied individually, stressors increased the number of sympathetic fibers in comparison to controls, whereas in combination they decreased it. The activity of IBAT monoamine oxidase-A (MAO-A) decreased under the influence of each stressor independent of its type or duration. SOD activity coincided with MAO-A decrement, whereas CAT activity had an opposite pattern of changes. We conclude that acute and chronic exposure to non-thermal stressors, immobilization and isolation, respectively, affect the metabolic potential of rat IBAT, judging by the increase in UCP-1 content and sympathetic outflow. However, when acute immobilization was applied as a novel stressor to previously chronically isolated animals, an increase in the UCP-1 content was accompanied by a lower IBAT sympathetic outflow, suggesting that IBAT metabolic function under various stress condition is not solely dependent on SNS activity. T2 - Archives of Biological Sciences T1 - Single and Combined Effects of Acute and Chronic Non-Thermal Stressors on Rat Interscapular Brown Adipose Tissue Metabolic Activity VL - 65 IS - 3 SP - 919 EP - 927 DO - 10.2298/ABS1303919C ER -
@article{ author = "Cvijic, Gordana and Lakić, Iva and Vujovic, R. and Jasnic, N. and Djurasevic, S. and Dronjak, Slađana and Đorđević, Jelena D.", year = "2013", abstract = "The aim of this study was to examine whether the thermogenic potential of rat interscapular brown adipose tissue (IBAT) changes in response to acute and/or chronic exposure to non-thermal stressors (immobilization and isolation), by measuring the uncoupling protein 1 (UCP-1) content, MAO-A, SOD and CAT activities, as well as the number of IBAT sympathetic noradrenaline-containing nerve fibers. Both acute immobilization (2 h) and chronic isolation (21 days), as well as their combined effects, significantly increased the IBAT UCP-1 content in comparison to non-stressed animals. When applied individually, stressors increased the number of sympathetic fibers in comparison to controls, whereas in combination they decreased it. The activity of IBAT monoamine oxidase-A (MAO-A) decreased under the influence of each stressor independent of its type or duration. SOD activity coincided with MAO-A decrement, whereas CAT activity had an opposite pattern of changes. We conclude that acute and chronic exposure to non-thermal stressors, immobilization and isolation, respectively, affect the metabolic potential of rat IBAT, judging by the increase in UCP-1 content and sympathetic outflow. However, when acute immobilization was applied as a novel stressor to previously chronically isolated animals, an increase in the UCP-1 content was accompanied by a lower IBAT sympathetic outflow, suggesting that IBAT metabolic function under various stress condition is not solely dependent on SNS activity.", journal = "Archives of Biological Sciences", title = "Single and Combined Effects of Acute and Chronic Non-Thermal Stressors on Rat Interscapular Brown Adipose Tissue Metabolic Activity", volume = "65", number = "3", pages = "919-927", doi = "10.2298/ABS1303919C" }
Cvijic, G., Lakić, I., Vujovic, R., Jasnic, N., Djurasevic, S., Dronjak, S.,& Đorđević, J. D.. (2013). Single and Combined Effects of Acute and Chronic Non-Thermal Stressors on Rat Interscapular Brown Adipose Tissue Metabolic Activity. in Archives of Biological Sciences, 65(3), 919-927. https://doi.org/10.2298/ABS1303919C
Cvijic G, Lakić I, Vujovic R, Jasnic N, Djurasevic S, Dronjak S, Đorđević JD. Single and Combined Effects of Acute and Chronic Non-Thermal Stressors on Rat Interscapular Brown Adipose Tissue Metabolic Activity. in Archives of Biological Sciences. 2013;65(3):919-927. doi:10.2298/ABS1303919C .
Cvijic, Gordana, Lakić, Iva, Vujovic, R., Jasnic, N., Djurasevic, S., Dronjak, Slađana, Đorđević, Jelena D., "Single and Combined Effects of Acute and Chronic Non-Thermal Stressors on Rat Interscapular Brown Adipose Tissue Metabolic Activity" in Archives of Biological Sciences, 65, no. 3 (2013):919-927, https://doi.org/10.2298/ABS1303919C . .