Show simple item record

dc.creatorRomić, Snježana Đ.
dc.creatorTepavčević, Snežana
dc.creatorŽakula, Zorica
dc.creatorMilosavljević, Tijana
dc.creatorStojiljković, Mojca D.
dc.creatorŽivković, Maja
dc.creatorPopović, Milan
dc.creatorStanković, Aleksandra
dc.creatorKorićanac, Goran
dc.date.accessioned2018-03-01T23:26:55Z
dc.date.available2018-03-01T23:26:55Z
dc.date.issued2013
dc.identifier.issn0007-1145 (print)
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/5513
dc.description.abstractFructose-rich diets (FRD) cause cardiac insulin resistance manifested by impairment of Akt/endothelial NO synthase (eNOS) signalling. In contrast, oestradiol (E2) activates this signalling pathway in the heart. To study the ability of E2 to revert the detrimental effect of fructose on cardiac Akt/eNOS, female rats were subjected to a FRD and ovariectomy followed with or without E2 replacement. We also analysed the effects of the FRD and E2 on cardiac extracellular signal-regulated kinase (Erk 1/2) signalling related to their role in cardiac hypertrophy development. Expression of Akt, eNOS and Erk 1/2, as well as regulatory phosphorylations of these molecules were determined. The protein expression of cardiac Akt and eNOS was not affected by the diet or E2 treatment. However, the FRD was accompanied by a decrease in Akt phosphorylation at Ser(473) and Thr(308), and eNOS at Ser(1177), while the phosphorylation of eNOS at Thr(495) was increased. E2 replacement in ovariectomised fructose-fed rats caused a reversion of the diet effect on Akt and eNOS serine phosphorylation, but mostly had no effect on threonine phosphorylation of the molecules. The FRD and E2 treatment did not influence Erk 1/2 expression and phosphorylation and heart mass as well. The data show that E2 selectively suppress the negative effects of a FRD on Akt/eNOS signalling and probably point to the different effects of E2 on kinase/phosphatase pathways responsible for phosphorylation/dephosphorylation of Akt and eNOS. Furthermore, the results suggest that the heart of females in the reproductive period is partially protected against the damaging effects of increased fructose intake.en
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41009/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175085/RS//
dc.rightsopenAccessen
dc.sourceBritish Journal of Nutritionen
dc.subjectFructoseen
dc.subjectOestradiolen
dc.subjectHearten
dc.subjectProtein kinase Ben
dc.subjectEndothelial nitric oxide synthaseen
dc.titleDoes oestradiol attenuate the damaging effects of a fructose-rich diet on cardiac Akt/endothelial nitric oxide synthase signalling?en
dc.typearticleen
dcterms.abstractПоповиц, Милан; Ромић Сњежана; Корићанац Горан; Станковић Aлександра; Живковић Маја; Закула, Зорица; Милосављевиц, Тијана; Стојиљковић Мојца; Тепавчевић Снежана;
dc.citation.volume109
dc.citation.issue11
dc.citation.spage1940
dc.citation.epage1948
dc.identifier.wos000319126700003
dc.identifier.doi10.1017/S0007114512004114
dc.citation.rankM21
dc.identifier.pmid23069112
dc.identifier.scopus2-s2.0-84877995385
dc.identifier.fulltexthttp://vinar.vin.bg.ac.rs//bitstream/id/6335/does_oestradiol_attenuate_the_damaging_effects_of_a_fructoserich_diet_on_cardiac_aktendothelial_nitric_oxide_synthase_signalling.pdf


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record