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dc.creatorKeta, Otilija D.
dc.creatorTodorović, Danijela V.
dc.creatorPopović, Nataša M.
dc.creatorKorićanac, Lela
dc.creatorCuttone, Giacomo
dc.creatorPetrović, Ivan M.
dc.creatorRistić-Fira, Aleksandra
dc.date.accessioned2018-03-01T23:21:13Z
dc.date.available2018-03-01T23:21:13Z
dc.date.issued2014
dc.identifier.issn1734-1922 (print)
dc.identifier.issn1896-9151 (electronic)
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/5447
dc.description.abstractIntroduction: Proton radiation offers physical advantages over conventional radiation. Radiosensitivity of human 59M ovarian cancer and HTB140 melanoma cells was investigated after exposure to gamma-rays and protons. Material and methods: Irradiations were performed in the middle of a 62 MeV therapeutic proton spread out Bragg peak with doses ranging from 2 to 16 Gy. The mean energy of protons was 34.88+/-2.15 MeV, corresponding to the linear energy transfer of 4.7+/-0.2 keV/mu m. Irradiations with gamma-rays were performed using the same doses. Viability, proliferation and survival were assessed 7 days after both types of irradiation while analyses of cell cycle and apoptosis were performed 48 h after irradiation. Results: Results showed that gamma-rays and protons reduced the number of viable cells for both cell lines, with stronger inactivation achieved after irradiation with protons. Surviving fractions for 59M were 0.91+/-0.01 for gamma-rays and 0.81+/-0.01 for protons, while those for HTB140 cells were 0.93+/-0.01 for gamma-rays and 0.86+/-0.01 for protons. Relative biological effectiveness of protons, being 2.47+/-0.22 for 59M and 2.08+/-0.36 for HTB140, indicated that protons provoked better cell elimination than gamma-rays. After proton irradiation proliferation capacity of the two cell lines was slightly higher as compared to gamma-rays. Proliferation was higher for 59M than for HTB140 cells after both types of irradiation. Induction of apoptosis and G2 arrest detected after proton irradiation were more prominent in 59M cells. Conclusions: The obtained results suggest that protons exert better antitumour effects on ovarian carcinoma and melanoma cells than gamma-rays. The dissimilar response of these cells to radiation is related to their different features.en
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173046/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/171019/RS//
dc.relationIstituto Nazionale di Fisica Nucleare, Laboratori Nazionali del Sud, Italy
dc.rightsopenAccessen
dc.sourceArchives of Medical Scienceen
dc.subjectapoptosisen
dc.subjectcell cycleen
dc.subjectgamma-raysen
dc.subjectmelanomaen
dc.subjectprotonsen
dc.subjectovarian carcinomaen
dc.titleRadiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protonsen
dc.typearticleen
dcterms.abstractТодоровиц, Данијела; Поповић Наташа; Корићанац Лела; Цуттоне, Гиацомо; Петровић Иван; Кета, Отилија; Ристић-Фира Aлександра;
dc.citation.volume10
dc.citation.issue3
dc.citation.spage578
dc.citation.epage586
dc.identifier.wos000338485400024
dc.identifier.doi10.5114/aoms.2014.43751
dc.citation.rankM22
dc.identifier.pmid25097591
dc.identifier.scopus2-s2.0-84903584291
dc.identifier.fulltexthttp://vinar.vin.bg.ac.rs//bitstream/id/6336/AOMS_Art_23023-10.pdf


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