Phosphorylation of leukocyte glucocorticoid receptor in patients with current episode of major depressive disorder
Само за регистроване кориснике
2013
Аутори
Simić, IvaMarić, Nađa P.
Mitić, Miloš
Soldatovic, Ivan
Pavlović, Zorana
Mihaljević, Marina
Andrić, Sanja
Radoičić, Marija B.
Adžić, Miroslav
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The impaired glucocorticoid receptor (GR) signaling has long been considered one of the cornerstones in understanding the pathophysiology of depression. Since the phosphorylation of GR is very important for GR function, in this study we investigated whether GR phosphorylation at serine 211 (pGR-S211) and serine 226 (pGR-S226) is altered in patients with current episode of major depressive disorder (MDD). Particularly, in 30 MDD patients and 35 controls we assessed the levels of nuclear total GR (tGR), pGR-S211 and pGR-S226 in peripheral blood mononuclear cells (PBMC) using Western blot technique, along with plasma cortisol concentrations from the same blood samples. Our results demonstrated increased phosphorylation of GR at S226 (p LT 0.001) and, to a less extent, at S211 (p LT 0.05) in MDD patients compared to controls. Consequently, the pGR-S211/pGR-S226 ratio was decreased (p LT 0.05) implying reduced transcriptional activity of GR in MDD patients. MDD subjects had higher cortisol ...levels than controls and cortisol concentrations were positively correlated with PBMC pGR-S226 levels from the same blood samples. There was no difference in the levels of tGR between MDD and control subjects. The study showed that altered phosphorylation of GR could contribute to impaired GR function related to the pathophysiology of depression. (c) 2012 Elsevier Inc. All rights reserved.
Кључне речи:
Glucocorticoid receptor / Major depressive disorder / PhosphorylationИзвор:
Progress in Neuro-psychopharmacology and Biological Psychiatry, 2013, 40, 281-285Финансирање / пројекти:
- Дефинисање кластера молекулских биомаркера за побољшану дијагностику и терапију поремећаја расположења (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41029)
DOI: 10.1016/j.pnpbp.2012.10.021
ISSN: 0278-5846
PubMed: 23123359
WoS: 000315422500041
Scopus: 2-s2.0-84869451228
Колекције
Институција/група
VinčaTY - JOUR AU - Simić, Iva AU - Marić, Nađa P. AU - Mitić, Miloš AU - Soldatovic, Ivan AU - Pavlović, Zorana AU - Mihaljević, Marina AU - Andrić, Sanja AU - Radoičić, Marija B. AU - Adžić, Miroslav PY - 2013 UR - https://vinar.vin.bg.ac.rs/handle/123456789/5340 AB - The impaired glucocorticoid receptor (GR) signaling has long been considered one of the cornerstones in understanding the pathophysiology of depression. Since the phosphorylation of GR is very important for GR function, in this study we investigated whether GR phosphorylation at serine 211 (pGR-S211) and serine 226 (pGR-S226) is altered in patients with current episode of major depressive disorder (MDD). Particularly, in 30 MDD patients and 35 controls we assessed the levels of nuclear total GR (tGR), pGR-S211 and pGR-S226 in peripheral blood mononuclear cells (PBMC) using Western blot technique, along with plasma cortisol concentrations from the same blood samples. Our results demonstrated increased phosphorylation of GR at S226 (p LT 0.001) and, to a less extent, at S211 (p LT 0.05) in MDD patients compared to controls. Consequently, the pGR-S211/pGR-S226 ratio was decreased (p LT 0.05) implying reduced transcriptional activity of GR in MDD patients. MDD subjects had higher cortisol levels than controls and cortisol concentrations were positively correlated with PBMC pGR-S226 levels from the same blood samples. There was no difference in the levels of tGR between MDD and control subjects. The study showed that altered phosphorylation of GR could contribute to impaired GR function related to the pathophysiology of depression. (c) 2012 Elsevier Inc. All rights reserved. T2 - Progress in Neuro-psychopharmacology and Biological Psychiatry T1 - Phosphorylation of leukocyte glucocorticoid receptor in patients with current episode of major depressive disorder VL - 40 SP - 281 EP - 285 DO - 10.1016/j.pnpbp.2012.10.021 ER -
@article{ author = "Simić, Iva and Marić, Nađa P. and Mitić, Miloš and Soldatovic, Ivan and Pavlović, Zorana and Mihaljević, Marina and Andrić, Sanja and Radoičić, Marija B. and Adžić, Miroslav", year = "2013", abstract = "The impaired glucocorticoid receptor (GR) signaling has long been considered one of the cornerstones in understanding the pathophysiology of depression. Since the phosphorylation of GR is very important for GR function, in this study we investigated whether GR phosphorylation at serine 211 (pGR-S211) and serine 226 (pGR-S226) is altered in patients with current episode of major depressive disorder (MDD). Particularly, in 30 MDD patients and 35 controls we assessed the levels of nuclear total GR (tGR), pGR-S211 and pGR-S226 in peripheral blood mononuclear cells (PBMC) using Western blot technique, along with plasma cortisol concentrations from the same blood samples. Our results demonstrated increased phosphorylation of GR at S226 (p LT 0.001) and, to a less extent, at S211 (p LT 0.05) in MDD patients compared to controls. Consequently, the pGR-S211/pGR-S226 ratio was decreased (p LT 0.05) implying reduced transcriptional activity of GR in MDD patients. MDD subjects had higher cortisol levels than controls and cortisol concentrations were positively correlated with PBMC pGR-S226 levels from the same blood samples. There was no difference in the levels of tGR between MDD and control subjects. The study showed that altered phosphorylation of GR could contribute to impaired GR function related to the pathophysiology of depression. (c) 2012 Elsevier Inc. All rights reserved.", journal = "Progress in Neuro-psychopharmacology and Biological Psychiatry", title = "Phosphorylation of leukocyte glucocorticoid receptor in patients with current episode of major depressive disorder", volume = "40", pages = "281-285", doi = "10.1016/j.pnpbp.2012.10.021" }
Simić, I., Marić, N. P., Mitić, M., Soldatovic, I., Pavlović, Z., Mihaljević, M., Andrić, S., Radoičić, M. B.,& Adžić, M.. (2013). Phosphorylation of leukocyte glucocorticoid receptor in patients with current episode of major depressive disorder. in Progress in Neuro-psychopharmacology and Biological Psychiatry, 40, 281-285. https://doi.org/10.1016/j.pnpbp.2012.10.021
Simić I, Marić NP, Mitić M, Soldatovic I, Pavlović Z, Mihaljević M, Andrić S, Radoičić MB, Adžić M. Phosphorylation of leukocyte glucocorticoid receptor in patients with current episode of major depressive disorder. in Progress in Neuro-psychopharmacology and Biological Psychiatry. 2013;40:281-285. doi:10.1016/j.pnpbp.2012.10.021 .
Simić, Iva, Marić, Nađa P., Mitić, Miloš, Soldatovic, Ivan, Pavlović, Zorana, Mihaljević, Marina, Andrić, Sanja, Radoičić, Marija B., Adžić, Miroslav, "Phosphorylation of leukocyte glucocorticoid receptor in patients with current episode of major depressive disorder" in Progress in Neuro-psychopharmacology and Biological Psychiatry, 40 (2013):281-285, https://doi.org/10.1016/j.pnpbp.2012.10.021 . .