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dc.creatorGavrilović, Ljubica
dc.creatorMandušić, Vesna
dc.creatorStojiljković, Vesna
dc.creatorKasapović, Jelena
dc.creatorStojiljkovic, S.
dc.creatorPajović, Snežana B.
dc.creatorDronjak, Slađana
dc.date.accessioned2018-03-01T22:52:29Z
dc.date.available2018-03-01T22:52:29Z
dc.date.issued2012
dc.identifier.issn0393-974X (print)
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/5112
dc.description.abstractThe sympathoneural system has a profound influence on the heart function. Sympathetic neurons are the major contributors to the huge rise of circulating noradrenaline (NA) level in response to stressful stimuli. Treadmill training in rats is forced exercise which has the propensity to induce both psychological and physical stress. The aim of this study is to examine how chronic forced running (CFR) affects the expression of catecholamine biosynthetic enzymes (tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT)) and cAMP response element-binding (CREB) in stellate ganglia, as well as the concentrations of catecholamines, adrenocorticotropic hormone (ACTH) and corticosterone (CORT) in the plasma of rats. Also, we investigated how the additional acute immobilization stress changes the mentioned parameters. The rat training program consisted of 12 weeks running on a treadmill (20 m/min, 20 min/day). We found that CFR increases TH and DBH mRNA and protein levels in stellate ganglia, which is followed by increased NA concentration in the plasma. CFR reduces the level of PNMT m RNA, while the level of PNMT protein remains unchanged in stellate ganglia. The increased expression of TH and DBH genes positively correlates with the expression of CREB in stellate ganglia and with plasma ACTH level, while reduced level of PNMT mRNA in stellate ganglia correlates with reduced plasma CORT level. The additional acute immobilization stress increased gene expression of catecholamine biosynthetic enzymes in stellate ganglia, as well as catecholamines, ACTH and CORT levels in the plasma. The results presented here suggest that the continuous increase of the noradrenaline biosynthetic enzyme expression in stellate ganglia due to CFR may play a role in growing risk of cardiovascular diseases.en
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41027/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41022/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173044/RS//
dc.rightsrestrictedAccessen
dc.sourceJournal of Biological Regulators and Homeostatic Agentsen
dc.subjectcatecholamineen
dc.subjectchronic forced runningen
dc.subjectimmobilization stressen
dc.subjectstellate gangliaen
dc.subjectqRT-PCRen
dc.titleEffect of Chronic Forced Running on Gene Expression of Catecholamine Biosynthetic Enzymes in Stellate Ganglia of Ratsen
dc.typearticleen
dcterms.abstractГавриловић Љубица; Стојиљковић Весна; Пајовиц, С. Б.; Стојиљковиц, С.; Касаповић Јелена; Дроњак Слађана; Мандушић Весна;
dc.citation.volume26
dc.citation.issue3
dc.citation.spage367
dc.citation.epage377
dc.identifier.wos000310192000007
dc.identifier.pmid23034256


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