Electrochemical Separation of 90-Yttrium in the Electrochemical Sr-90/Y-90 Generator and Its Use for Radiolabelling of Dota-Conjugated Somatostatin Analog [Dota(0), Tyr(3)] Octreotate
Abstract
Radiopharmaceuticals based on Y-90 are widely used in the treatment of malignant deseases. In order to meet the requirements for their future application, a Sr-90/Y-90 generator was developed and 90Y eluted from this locally produced generator was used for the radiolabelling of the DOTA-conjugated somatostatin analog [DOTA(0),Tyr(3)] octreotate and the preparation of [Y-90-DOTA(0),Tyr(3)] octreotate (Y-90-DOTATATE) for peptide receptore radionuclide therapy. Sr-90/Y-90 generator was based on the electrochemical separation of Y-90 from Sr-90 in a two-cycle electrolysis procedure. Three electrode cells were used to perform both electrolyses. In both cycles, working electrodes were kept on constant potential. The pH of the solution was adjusted to 2.7 of the value before the electrolyses. The radionucliclic purity of the Y-90 solution was analysed by ITLC and extraction paper chromatography. The labelling of peptide (100 mu g DOTATATE) with (YCl3)-Y-90 was performed at 95 degrees C for 30... minutes. Radiochemical purity was determined by HPLC and chromatographic separation, using a solid SepPak C-18 column. Results obtained confirmed the efficiency of our electrochemical separation technique and quality control methods for Y-90. The achieved efficiency of the Sr-90/Y-90 generator above 96% of the theoretical value represents a good basis for the further development of this generator. The labelling of the DOTATATE with Y-90 exhibited a high efficiency, too: there was less than 1% of Y-90(3+) in the Y-90-DOTATATE.
Keywords:
radionuclide therapy Y-90 / Sr-90/Y-90 generator / radiolabelling / Y-90-DOTATATESource:
Nuclear technology and radiation protection, 2012, 27, 3, 260-268Funding / projects:
- Magnetic and radionuclide labeled nanostructured materials for medical applications (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45015)
- Ministry of Education and Sciences of the Republic of Serbia
DOI: 10.2298/NTRP1203260P
ISSN: 1451-3994
WoS: 000309481800007
Scopus: 2-s2.0-84867915148
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VinčaTY - JOUR AU - Petrović, Đorđe AU - Nikolić, Nadežda S. AU - Stanković, Dragana AU - Đokić, Divna Đ. PY - 2012 UR - https://vinar.vin.bg.ac.rs/handle/123456789/5069 AB - Radiopharmaceuticals based on Y-90 are widely used in the treatment of malignant deseases. In order to meet the requirements for their future application, a Sr-90/Y-90 generator was developed and 90Y eluted from this locally produced generator was used for the radiolabelling of the DOTA-conjugated somatostatin analog [DOTA(0),Tyr(3)] octreotate and the preparation of [Y-90-DOTA(0),Tyr(3)] octreotate (Y-90-DOTATATE) for peptide receptore radionuclide therapy. Sr-90/Y-90 generator was based on the electrochemical separation of Y-90 from Sr-90 in a two-cycle electrolysis procedure. Three electrode cells were used to perform both electrolyses. In both cycles, working electrodes were kept on constant potential. The pH of the solution was adjusted to 2.7 of the value before the electrolyses. The radionucliclic purity of the Y-90 solution was analysed by ITLC and extraction paper chromatography. The labelling of peptide (100 mu g DOTATATE) with (YCl3)-Y-90 was performed at 95 degrees C for 30 minutes. Radiochemical purity was determined by HPLC and chromatographic separation, using a solid SepPak C-18 column. Results obtained confirmed the efficiency of our electrochemical separation technique and quality control methods for Y-90. The achieved efficiency of the Sr-90/Y-90 generator above 96% of the theoretical value represents a good basis for the further development of this generator. The labelling of the DOTATATE with Y-90 exhibited a high efficiency, too: there was less than 1% of Y-90(3+) in the Y-90-DOTATATE. T2 - Nuclear technology and radiation protection T1 - Electrochemical Separation of 90-Yttrium in the Electrochemical Sr-90/Y-90 Generator and Its Use for Radiolabelling of Dota-Conjugated Somatostatin Analog [Dota(0), Tyr(3)] Octreotate VL - 27 IS - 3 SP - 260 EP - 268 DO - 10.2298/NTRP1203260P ER -
@article{ author = "Petrović, Đorđe and Nikolić, Nadežda S. and Stanković, Dragana and Đokić, Divna Đ.", year = "2012", abstract = "Radiopharmaceuticals based on Y-90 are widely used in the treatment of malignant deseases. In order to meet the requirements for their future application, a Sr-90/Y-90 generator was developed and 90Y eluted from this locally produced generator was used for the radiolabelling of the DOTA-conjugated somatostatin analog [DOTA(0),Tyr(3)] octreotate and the preparation of [Y-90-DOTA(0),Tyr(3)] octreotate (Y-90-DOTATATE) for peptide receptore radionuclide therapy. Sr-90/Y-90 generator was based on the electrochemical separation of Y-90 from Sr-90 in a two-cycle electrolysis procedure. Three electrode cells were used to perform both electrolyses. In both cycles, working electrodes were kept on constant potential. The pH of the solution was adjusted to 2.7 of the value before the electrolyses. The radionucliclic purity of the Y-90 solution was analysed by ITLC and extraction paper chromatography. The labelling of peptide (100 mu g DOTATATE) with (YCl3)-Y-90 was performed at 95 degrees C for 30 minutes. Radiochemical purity was determined by HPLC and chromatographic separation, using a solid SepPak C-18 column. Results obtained confirmed the efficiency of our electrochemical separation technique and quality control methods for Y-90. The achieved efficiency of the Sr-90/Y-90 generator above 96% of the theoretical value represents a good basis for the further development of this generator. The labelling of the DOTATATE with Y-90 exhibited a high efficiency, too: there was less than 1% of Y-90(3+) in the Y-90-DOTATATE.", journal = "Nuclear technology and radiation protection", title = "Electrochemical Separation of 90-Yttrium in the Electrochemical Sr-90/Y-90 Generator and Its Use for Radiolabelling of Dota-Conjugated Somatostatin Analog [Dota(0), Tyr(3)] Octreotate", volume = "27", number = "3", pages = "260-268", doi = "10.2298/NTRP1203260P" }
Petrović, Đ., Nikolić, N. S., Stanković, D.,& Đokić, D. Đ.. (2012). Electrochemical Separation of 90-Yttrium in the Electrochemical Sr-90/Y-90 Generator and Its Use for Radiolabelling of Dota-Conjugated Somatostatin Analog [Dota(0), Tyr(3)] Octreotate. in Nuclear technology and radiation protection, 27(3), 260-268. https://doi.org/10.2298/NTRP1203260P
Petrović Đ, Nikolić NS, Stanković D, Đokić DĐ. Electrochemical Separation of 90-Yttrium in the Electrochemical Sr-90/Y-90 Generator and Its Use for Radiolabelling of Dota-Conjugated Somatostatin Analog [Dota(0), Tyr(3)] Octreotate. in Nuclear technology and radiation protection. 2012;27(3):260-268. doi:10.2298/NTRP1203260P .
Petrović, Đorđe, Nikolić, Nadežda S., Stanković, Dragana, Đokić, Divna Đ., "Electrochemical Separation of 90-Yttrium in the Electrochemical Sr-90/Y-90 Generator and Its Use for Radiolabelling of Dota-Conjugated Somatostatin Analog [Dota(0), Tyr(3)] Octreotate" in Nuclear technology and radiation protection, 27, no. 3 (2012):260-268, https://doi.org/10.2298/NTRP1203260P . .