Electrochemical Separation of 90-Yttrium in the Electrochemical Sr-90/Y-90 Generator and Its Use for Radiolabelling of Dota-Conjugated Somatostatin Analog [Dota(0), Tyr(3)] Octreotate
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Radiopharmaceuticals based on Y-90 are widely used in the treatment of malignant deseases. In order to meet the requirements for their future application, a Sr-90/Y-90 generator was developed and 90Y eluted from this locally produced generator was used for the radiolabelling of the DOTA-conjugated somatostatin analog [DOTA(0),Tyr(3)] octreotate and the preparation of [Y-90-DOTA(0),Tyr(3)] octreotate (Y-90-DOTATATE) for peptide receptore radionuclide therapy. Sr-90/Y-90 generator was based on the electrochemical separation of Y-90 from Sr-90 in a two-cycle electrolysis procedure. Three electrode cells were used to perform both electrolyses. In both cycles, working electrodes were kept on constant potential. The pH of the solution was adjusted to 2.7 of the value before the electrolyses. The radionucliclic purity of the Y-90 solution was analysed by ITLC and extraction paper chromatography. The labelling of peptide (100 mu g DOTATATE) with (YCl3)-Y-90 was performed at 95 degrees C for 30... minutes. Radiochemical purity was determined by HPLC and chromatographic separation, using a solid SepPak C-18 column. Results obtained confirmed the efficiency of our electrochemical separation technique and quality control methods for Y-90. The achieved efficiency of the Sr-90/Y-90 generator above 96% of the theoretical value represents a good basis for the further development of this generator. The labelling of the DOTATATE with Y-90 exhibited a high efficiency, too: there was less than 1% of Y-90(3+) in the Y-90-DOTATATE.
Keywords:radionuclide therapy Y-90 / Sr-90/Y-90 generator / radiolabelling / Y-90-DOTATATE
Source:Nuclear technology and radiation protection, 2012, 27, 3, 260-268
- Magnetic and radionuclide labeled nanostructured materials for medical applications (RS-45015)
- Ministry of Education and Sciences of the Republic of Serbia