Differential regulation of catecholamine synthesis and transport in rat adrenal medulla by fluoxetine treatment
Апстракт
We have recently shown that chronic fluoxetine treatment acted significantly increasing plasma norepinephrine and epinephrine concentrations both in control and chronically stressed adult male rats. However, possible effects of fluoxetine on catecholamine synthesis and re-uptake in adrenal medulla have been largely unknown. In the present study the effects of chronic fluoxetine treatment on tyrosine hydroxylase, a rate-limiting enzyme in catecholamine synthesis, as well as a norepinephrine transporter and vesicular monoamine transporter 2 gene expressions in adrenal medulla of animals exposed to chronic unpredictable mild stress (CUMS) for 4 weeks, were investigated. Gene expression analyses were performed using a real-time quantitative reverse transcription-PCR. Chronically stressed animals had increased tyrosine hydroxylase mRNA levels and decreased expression of both transporters. Fluoxetine increased tyrosine hydroxylase and decreased norepinephrine transporter gene expression in b...oth unstressed and CUMS rats. These findings suggest that chronic fluoxetine treatment increased plasma catecholamine levels by affecting opposing changes in catecholamine synthesis and uptake.
Кључне речи:
adrenal medulla / antidepressant / gene expression / norepinephrine transporter / tyrosine hydroxylaseИзвор:
Anais de Academia Brasileira de Ciencias, 2015, 87, 1, 343-350Финансирање / пројекти:
- Молекуларни механизми патофизиолошких промена у ћелијама централног нервног система и периферног ткива код сисара (RS-MESTD-Basic Research (BR or ON)-173044)
DOI: 10.1590/0001-3765201520130400
ISSN: 0001-3765; 1678-2690
PubMed: 25651155
WoS: 000351560600029
Scopus: 2-s2.0-84925943340
Колекције
Институција/група
VinčaTY - JOUR AU - Spasojević, Nataša AU - Jovanović, Predrag AU - Dronjak, Slađana PY - 2015 UR - https://vinar.vin.bg.ac.rs/handle/123456789/463 AB - We have recently shown that chronic fluoxetine treatment acted significantly increasing plasma norepinephrine and epinephrine concentrations both in control and chronically stressed adult male rats. However, possible effects of fluoxetine on catecholamine synthesis and re-uptake in adrenal medulla have been largely unknown. In the present study the effects of chronic fluoxetine treatment on tyrosine hydroxylase, a rate-limiting enzyme in catecholamine synthesis, as well as a norepinephrine transporter and vesicular monoamine transporter 2 gene expressions in adrenal medulla of animals exposed to chronic unpredictable mild stress (CUMS) for 4 weeks, were investigated. Gene expression analyses were performed using a real-time quantitative reverse transcription-PCR. Chronically stressed animals had increased tyrosine hydroxylase mRNA levels and decreased expression of both transporters. Fluoxetine increased tyrosine hydroxylase and decreased norepinephrine transporter gene expression in both unstressed and CUMS rats. These findings suggest that chronic fluoxetine treatment increased plasma catecholamine levels by affecting opposing changes in catecholamine synthesis and uptake. T2 - Anais de Academia Brasileira de Ciencias T1 - Differential regulation of catecholamine synthesis and transport in rat adrenal medulla by fluoxetine treatment VL - 87 IS - 1 SP - 343 EP - 350 DO - 10.1590/0001-3765201520130400 ER -
@article{ author = "Spasojević, Nataša and Jovanović, Predrag and Dronjak, Slađana", year = "2015", abstract = "We have recently shown that chronic fluoxetine treatment acted significantly increasing plasma norepinephrine and epinephrine concentrations both in control and chronically stressed adult male rats. However, possible effects of fluoxetine on catecholamine synthesis and re-uptake in adrenal medulla have been largely unknown. In the present study the effects of chronic fluoxetine treatment on tyrosine hydroxylase, a rate-limiting enzyme in catecholamine synthesis, as well as a norepinephrine transporter and vesicular monoamine transporter 2 gene expressions in adrenal medulla of animals exposed to chronic unpredictable mild stress (CUMS) for 4 weeks, were investigated. Gene expression analyses were performed using a real-time quantitative reverse transcription-PCR. Chronically stressed animals had increased tyrosine hydroxylase mRNA levels and decreased expression of both transporters. Fluoxetine increased tyrosine hydroxylase and decreased norepinephrine transporter gene expression in both unstressed and CUMS rats. These findings suggest that chronic fluoxetine treatment increased plasma catecholamine levels by affecting opposing changes in catecholamine synthesis and uptake.", journal = "Anais de Academia Brasileira de Ciencias", title = "Differential regulation of catecholamine synthesis and transport in rat adrenal medulla by fluoxetine treatment", volume = "87", number = "1", pages = "343-350", doi = "10.1590/0001-3765201520130400" }
Spasojević, N., Jovanović, P.,& Dronjak, S.. (2015). Differential regulation of catecholamine synthesis and transport in rat adrenal medulla by fluoxetine treatment. in Anais de Academia Brasileira de Ciencias, 87(1), 343-350. https://doi.org/10.1590/0001-3765201520130400
Spasojević N, Jovanović P, Dronjak S. Differential regulation of catecholamine synthesis and transport in rat adrenal medulla by fluoxetine treatment. in Anais de Academia Brasileira de Ciencias. 2015;87(1):343-350. doi:10.1590/0001-3765201520130400 .
Spasojević, Nataša, Jovanović, Predrag, Dronjak, Slađana, "Differential regulation of catecholamine synthesis and transport in rat adrenal medulla by fluoxetine treatment" in Anais de Academia Brasileira de Ciencias, 87, no. 1 (2015):343-350, https://doi.org/10.1590/0001-3765201520130400 . .