Oxidation of chlorpyrifos, azinphos-methyl and phorate by myeloperoxidase
Abstract
The present paper deals with the investigations of optimal conditions for the myeloperoxidase (MPO) mediated oxidation of chlorpyrifos, azinphos-methyl and phorate, organophosphorous pesticides (OPs) containing phosphorothionate group, from thio- to oxo-forms, in the presence of hydrogen peroxide. The aim of the work was to apply this oxidation method in the AChE based bioanalytical tests for OPs determination. The maximum concentration of oxo-forms for all tested pesticides was achieved after 10 min incubation of OPs in 50 mM phosphate buffer (pH 6.0) with 100 nM MPO in the presence of 50 mu M H(2)O(2). Optimal temperature for obtaining maximal concentration of oxo-forms was 37 degrees C. Only the parent compounds and their oxo-forms were identified chromatographically in the OPs samples after their exposure to MPO. Moreover, no hydrolysis products were detected in the time interval of 1 h after the MPO catalyzed reaction was stopped by catalase. The efficiency of OPs transformation f...rom thio- to oxo-forms was measured using acethylcholinesterase (AChE) test, by comparison of percent of AChE inhibition before and after exposure to the oxidized sample. (C) 2011 Elsevier Inc. All rights reserved.
Keywords:
Organophosphate / Pesticide / Oxidation / Myeloperoxidase / acetylcholinesteraseSource:
Pesticide Biochemistry and Physiology, 2011, 101, 3, 220-226Funding / projects:
- Studies of enzyme interactions with toxic and pharmacologically active molecules (RS-MESTD-Basic Research (BR or ON)-172023)
DOI: 10.1016/j.pestbp.2011.09.009
ISSN: 0048-3575
WoS: 000297879600012
Scopus: 2-s2.0-81555204344
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Institution/Community
VinčaTY - JOUR AU - Lazarević-Pašti, Tamara AU - Nastasijević, Branislav J. AU - Vasić, Vesna M. PY - 2011 UR - https://vinar.vin.bg.ac.rs/handle/123456789/4597 AB - The present paper deals with the investigations of optimal conditions for the myeloperoxidase (MPO) mediated oxidation of chlorpyrifos, azinphos-methyl and phorate, organophosphorous pesticides (OPs) containing phosphorothionate group, from thio- to oxo-forms, in the presence of hydrogen peroxide. The aim of the work was to apply this oxidation method in the AChE based bioanalytical tests for OPs determination. The maximum concentration of oxo-forms for all tested pesticides was achieved after 10 min incubation of OPs in 50 mM phosphate buffer (pH 6.0) with 100 nM MPO in the presence of 50 mu M H(2)O(2). Optimal temperature for obtaining maximal concentration of oxo-forms was 37 degrees C. Only the parent compounds and their oxo-forms were identified chromatographically in the OPs samples after their exposure to MPO. Moreover, no hydrolysis products were detected in the time interval of 1 h after the MPO catalyzed reaction was stopped by catalase. The efficiency of OPs transformation from thio- to oxo-forms was measured using acethylcholinesterase (AChE) test, by comparison of percent of AChE inhibition before and after exposure to the oxidized sample. (C) 2011 Elsevier Inc. All rights reserved. T2 - Pesticide Biochemistry and Physiology T1 - Oxidation of chlorpyrifos, azinphos-methyl and phorate by myeloperoxidase VL - 101 IS - 3 SP - 220 EP - 226 DO - 10.1016/j.pestbp.2011.09.009 ER -
@article{ author = "Lazarević-Pašti, Tamara and Nastasijević, Branislav J. and Vasić, Vesna M.", year = "2011", abstract = "The present paper deals with the investigations of optimal conditions for the myeloperoxidase (MPO) mediated oxidation of chlorpyrifos, azinphos-methyl and phorate, organophosphorous pesticides (OPs) containing phosphorothionate group, from thio- to oxo-forms, in the presence of hydrogen peroxide. The aim of the work was to apply this oxidation method in the AChE based bioanalytical tests for OPs determination. The maximum concentration of oxo-forms for all tested pesticides was achieved after 10 min incubation of OPs in 50 mM phosphate buffer (pH 6.0) with 100 nM MPO in the presence of 50 mu M H(2)O(2). Optimal temperature for obtaining maximal concentration of oxo-forms was 37 degrees C. Only the parent compounds and their oxo-forms were identified chromatographically in the OPs samples after their exposure to MPO. Moreover, no hydrolysis products were detected in the time interval of 1 h after the MPO catalyzed reaction was stopped by catalase. The efficiency of OPs transformation from thio- to oxo-forms was measured using acethylcholinesterase (AChE) test, by comparison of percent of AChE inhibition before and after exposure to the oxidized sample. (C) 2011 Elsevier Inc. All rights reserved.", journal = "Pesticide Biochemistry and Physiology", title = "Oxidation of chlorpyrifos, azinphos-methyl and phorate by myeloperoxidase", volume = "101", number = "3", pages = "220-226", doi = "10.1016/j.pestbp.2011.09.009" }
Lazarević-Pašti, T., Nastasijević, B. J.,& Vasić, V. M.. (2011). Oxidation of chlorpyrifos, azinphos-methyl and phorate by myeloperoxidase. in Pesticide Biochemistry and Physiology, 101(3), 220-226. https://doi.org/10.1016/j.pestbp.2011.09.009
Lazarević-Pašti T, Nastasijević BJ, Vasić VM. Oxidation of chlorpyrifos, azinphos-methyl and phorate by myeloperoxidase. in Pesticide Biochemistry and Physiology. 2011;101(3):220-226. doi:10.1016/j.pestbp.2011.09.009 .
Lazarević-Pašti, Tamara, Nastasijević, Branislav J., Vasić, Vesna M., "Oxidation of chlorpyrifos, azinphos-methyl and phorate by myeloperoxidase" in Pesticide Biochemistry and Physiology, 101, no. 3 (2011):220-226, https://doi.org/10.1016/j.pestbp.2011.09.009 . .