Response of Human HTB140 Melanoma Cells to Conventional Radiation and Hadrons
Аутори
Ristić-Fira, AleksandraTodorović, Danijela V.
Žakula, Jelena
Keta, Otilija D.
Cirrone, Giuseppe Antonio Pablo
Cuttone, Giacomo
Petrović, Ivan M.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Conventional radiotherapy with X-and gamma-rays is one of the common and effective treatments of cancer. High energy hadrons, i.e., charged particles like protons and (12)C ions, due to their specific physics and radiobiological advantages are increasingly used. In this study, effectiveness of different radiation types is evaluated on the radio-resistant human HTB140 melanoma cells. The cells were irradiated with gamma-rays, the 62 MeV protons at the Bragg peak and in the middle of the spread-out Bragg peak (SOBP), as well as with the 62 MeV/u (12)C ions. The doses ranged from 2 to 24 Gy. Cell survival and proliferation were assessed 7 days after irradiation, whereas apoptosis was evaluated after 48 h. The acquired results confirmed the high radio-resistance of cells, showing better effectiveness of protons than gamma-rays. The best efficiency was obtained with (12)C ions due to higher linear energy transfer. All analyzed radiation qualities reduced cell proliferation. The highest prol...iferation was detected for (12)C ions because of their large killing capacity followed by small induction of reparable lesions. This enabled unharmed cells to preserve proliferative activity. Irradiations with protons and (12)C ions revealed similar moderate pro-apoptotic ability that is in agreement with the level of cellular radio-resistance.
Кључне речи:
Protons / Carbon ions / HTB140 Melanoma / Survival / ProliferationИзвор:
Physiological Research, 2011, 60, S129-S135Финансирање / пројекти:
- Радиосензитивност хуманог генома (RS-MESTD-Basic Research (BR or ON)-173046)
- Физика високих енергија са детектором CMS (RS-MESTD-Basic Research (BR or ON)-171019)
- Istituto Nazionale di Fisica Nucleare, Laboratori Nazionali del Sud, Italy
Институција/група
VinčaTY - JOUR AU - Ristić-Fira, Aleksandra AU - Todorović, Danijela V. AU - Žakula, Jelena AU - Keta, Otilija D. AU - Cirrone, Giuseppe Antonio Pablo AU - Cuttone, Giacomo AU - Petrović, Ivan M. PY - 2011 UR - https://vinar.vin.bg.ac.rs/handle/123456789/4537 AB - Conventional radiotherapy with X-and gamma-rays is one of the common and effective treatments of cancer. High energy hadrons, i.e., charged particles like protons and (12)C ions, due to their specific physics and radiobiological advantages are increasingly used. In this study, effectiveness of different radiation types is evaluated on the radio-resistant human HTB140 melanoma cells. The cells were irradiated with gamma-rays, the 62 MeV protons at the Bragg peak and in the middle of the spread-out Bragg peak (SOBP), as well as with the 62 MeV/u (12)C ions. The doses ranged from 2 to 24 Gy. Cell survival and proliferation were assessed 7 days after irradiation, whereas apoptosis was evaluated after 48 h. The acquired results confirmed the high radio-resistance of cells, showing better effectiveness of protons than gamma-rays. The best efficiency was obtained with (12)C ions due to higher linear energy transfer. All analyzed radiation qualities reduced cell proliferation. The highest proliferation was detected for (12)C ions because of their large killing capacity followed by small induction of reparable lesions. This enabled unharmed cells to preserve proliferative activity. Irradiations with protons and (12)C ions revealed similar moderate pro-apoptotic ability that is in agreement with the level of cellular radio-resistance. T2 - Physiological Research T1 - Response of Human HTB140 Melanoma Cells to Conventional Radiation and Hadrons VL - 60 SP - S129 EP - S135 UR - https://hdl.handle.net/21.15107/rcub_vinar_4537 ER -
@article{ author = "Ristić-Fira, Aleksandra and Todorović, Danijela V. and Žakula, Jelena and Keta, Otilija D. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Petrović, Ivan M.", year = "2011", abstract = "Conventional radiotherapy with X-and gamma-rays is one of the common and effective treatments of cancer. High energy hadrons, i.e., charged particles like protons and (12)C ions, due to their specific physics and radiobiological advantages are increasingly used. In this study, effectiveness of different radiation types is evaluated on the radio-resistant human HTB140 melanoma cells. The cells were irradiated with gamma-rays, the 62 MeV protons at the Bragg peak and in the middle of the spread-out Bragg peak (SOBP), as well as with the 62 MeV/u (12)C ions. The doses ranged from 2 to 24 Gy. Cell survival and proliferation were assessed 7 days after irradiation, whereas apoptosis was evaluated after 48 h. The acquired results confirmed the high radio-resistance of cells, showing better effectiveness of protons than gamma-rays. The best efficiency was obtained with (12)C ions due to higher linear energy transfer. All analyzed radiation qualities reduced cell proliferation. The highest proliferation was detected for (12)C ions because of their large killing capacity followed by small induction of reparable lesions. This enabled unharmed cells to preserve proliferative activity. Irradiations with protons and (12)C ions revealed similar moderate pro-apoptotic ability that is in agreement with the level of cellular radio-resistance.", journal = "Physiological Research", title = "Response of Human HTB140 Melanoma Cells to Conventional Radiation and Hadrons", volume = "60", pages = "S129-S135", url = "https://hdl.handle.net/21.15107/rcub_vinar_4537" }
Ristić-Fira, A., Todorović, D. V., Žakula, J., Keta, O. D., Cirrone, G. A. P., Cuttone, G.,& Petrović, I. M.. (2011). Response of Human HTB140 Melanoma Cells to Conventional Radiation and Hadrons. in Physiological Research, 60, S129-S135. https://hdl.handle.net/21.15107/rcub_vinar_4537
Ristić-Fira A, Todorović DV, Žakula J, Keta OD, Cirrone GAP, Cuttone G, Petrović IM. Response of Human HTB140 Melanoma Cells to Conventional Radiation and Hadrons. in Physiological Research. 2011;60:S129-S135. https://hdl.handle.net/21.15107/rcub_vinar_4537 .
Ristić-Fira, Aleksandra, Todorović, Danijela V., Žakula, Jelena, Keta, Otilija D., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Petrović, Ivan M., "Response of Human HTB140 Melanoma Cells to Conventional Radiation and Hadrons" in Physiological Research, 60 (2011):S129-S135, https://hdl.handle.net/21.15107/rcub_vinar_4537 .