VinaR - Repository of the Vinča Nuclear Institute
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrilic)
    • Serbian (Latin)
  • Login
View Item 
  •   Vinar
  • Vinča
  • WoS Import
  • View Item
  •   Vinar
  • Vinča
  • WoS Import
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

A Bioinformatics Approach to Identify Natural Autoantibodies from Healthy Blood Donors Sera Reactive with the HCV NS5A-Derived Peptide by Immunoassay

No Thumbnail
Authors
Vasiljevic, Nada
Veljković, Nevena V.
Kosec, Tatjana
Ma, Xue-Zhong
Glišić, Sanja
Prljić, Jelena
Vujicic, Ana Djordjevic
Markovic, Ljiljana
Branch, Donald R.
Article
Metadata
Show full item record
Abstract
Natural autoantibodies (NAbs) are continually produced throughout life and have an ability to recognize self and altered self, as well as foreign antigens, by recognizing cellular pattern recognition receptors. Sometimes NAb specificity demonstrates overlap between human and pathologic proteomes. This information can be useful in selecting target sequences for screening purposes. In this study we undertook a multi-step bioinformatics search to predict a virus-derived peptide that can be recognized by NAbs in sera of uninfected individuals. We selected protein hepatitis C virus (HCV) NS5A as a target sequence, motivated by the fact that the HCV proteome is characterized by extensive sequence similarities to the human proteome, and because screening for anti-HCV antibodies, including anti-NS5A, is important clinically, particularly in screening of potential blood donors. The virus-specific peptide P1, and the homologous human peptide derived from enzyme-inducible nitric oxide synthase (i...NOS), P2, exhibiting not only simple homology, but also complementarities of physicochemical patterns, were synthesized and 80 HCV-negative and 50 HCV-positive blood donor sera were tested by ELISA. These peptides reacted similarly (p LT 0.001) with HCV-negative sera, and in several cases the measured reactivity was significantly above the cut-off value of commercial anti-HCV screening assays. In HCV-positive sera, the titers of antibodies reactive with analyzed HCV NS5A peptide were not significantly increased (p LT 0.001) compared to host peptide, the implications of which are unclear, but may be consistent with these antibodies being naturally produced. Finally, we extended our bioinformatics analyses to the dataset of human self-binding sequences, and propose a general approach for the selection of specific diagnostic and screening antigens for use in immunoassays.

Source:
Viral Immunology, 2011, 24, 2, 69-76
Projects:
  • Application of the EIIP/ISM bioinformatics platform in discovery of novel therapeutic targets and potential therapeutic molecules (RS-173001)

DOI: 10.1089/vim.2010.0107

ISSN: 0882-8245 (print); 1557-8976 (electronic)

PubMed: 21449717

WoS: 000288983000002

Scopus: 2-s2.0-79953252853
[ Google Scholar ]
URI
http://vinar.vin.bg.ac.rs/handle/123456789/4259
Collections
  • WoS Import
Institution
Vinča

DSpace software copyright © 2002-2015  DuraSpace
About VinaR - Repository of the Vinča Institute of Nuclear Sciences | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceInstitutionsAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About VinaR - Repository of the Vinča Institute of Nuclear Sciences | Send Feedback

OpenAIRERCUB