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dc.creatorKorićanac, Goran
dc.creatorTepavčević, Snežana
dc.creatorŽakula, Zorica
dc.creatorMilosavljević, Tijana
dc.creatorStojiljković, Mojca D.
dc.creatorIsenović, Esma R.
dc.identifier.issn0014-2999 (print)
dc.description.abstractInsulin and estradiol share some of signaling pathways and regulate same target molecules exerting mostly beneficial cardiac effects. in order to study their cardiac interaction, ovariectomized female rats were treated with hormones, separately or simultaneously (20,30 or 40 min before analysis), and the phosphorylations of protein kinase B (Akt), extracellular signal-regulated kinases 1 and 2 (ERK 1/2), endothelial nitric oxide synthase (eNOS) were analyzed, as well as the plasma membrane content of alpha 2 subunit of Na+/K+-ATPase. Insulin, particularly, and estradiol stimulate Ser(473) Akt phosphorylation. The combined treatment was stimulatory, but less than insulin alone was. The general increase of Thr(308) Akt phosphorylation by insulin was stronger than at Ser(473) and reduced in the presence of estradiol, which also stimulated this phosphorylation given alone. The estradiol induction of ERK 1/2 phosphorylation was inverted to the decrease by the combined treatment, while insulin had no effect. Only insulin increased the plasma membrane content of alpha 2. Estradiol did increase the phosphorylation of eNOS, whereas the insulin effect was controversial. The effect of the combined treatment on target molecules was generally opposite to single hormone treatment. In summary, both hormones exerted an effect on Akt phosphorylation, but only estradiol stimulated ERK 1/2 phosphorylation. The alpha 2 plasma membrane content was increased only by insulin, while estradiol increased eNOS phosphorylation more consistently. Finally, if these hormones were administered together, it seems that they disturb each other in having a full effect on cardiac Akt, ERK 1/2, and downstream effectors, eNOS and Na+/K+-ATPase. (c) 2011 Elsevier B.V. All rights reserved.en
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41009/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173033/RS//
dc.sourceEuropean Journal of Pharmacologyen
dc.subjectProtein kinase Ben
dc.subjectExtracellular signal-regulated MAP kinaseen
dc.subjectNitric oxide synthase type IIIen
dc.subjectPotassium ATPaseen
dc.titleInterference between insulin and estradiol signaling pathways in the regulation of cardiac eNOS and Na+/K+-ATPaseen
dcterms.abstractКорићанац Горан; Тепавчевић Снежана; Стојиљковић Мојца; Исеновић Есма; Закула, Зорица; Милосављевиц, Тијана;

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