Interference between insulin and estradiol signaling pathways in the regulation of cardiac eNOS and Na+/K+-ATPase
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Korićanac, GoranTepavčević, Snežana
Žakula, Zorica
Milosavljević, Tijana
Stojiljković, Mojca D.
Isenović, Esma R.
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Insulin and estradiol share some of signaling pathways and regulate same target molecules exerting mostly beneficial cardiac effects. in order to study their cardiac interaction, ovariectomized female rats were treated with hormones, separately or simultaneously (20,30 or 40 min before analysis), and the phosphorylations of protein kinase B (Akt), extracellular signal-regulated kinases 1 and 2 (ERK 1/2), endothelial nitric oxide synthase (eNOS) were analyzed, as well as the plasma membrane content of alpha 2 subunit of Na+/K+-ATPase. Insulin, particularly, and estradiol stimulate Ser(473) Akt phosphorylation. The combined treatment was stimulatory, but less than insulin alone was. The general increase of Thr(308) Akt phosphorylation by insulin was stronger than at Ser(473) and reduced in the presence of estradiol, which also stimulated this phosphorylation given alone. The estradiol induction of ERK 1/2 phosphorylation was inverted to the decrease by the combined treatment, while insul...in had no effect. Only insulin increased the plasma membrane content of alpha 2. Estradiol did increase the phosphorylation of eNOS, whereas the insulin effect was controversial. The effect of the combined treatment on target molecules was generally opposite to single hormone treatment. In summary, both hormones exerted an effect on Akt phosphorylation, but only estradiol stimulated ERK 1/2 phosphorylation. The alpha 2 plasma membrane content was increased only by insulin, while estradiol increased eNOS phosphorylation more consistently. Finally, if these hormones were administered together, it seems that they disturb each other in having a full effect on cardiac Akt, ERK 1/2, and downstream effectors, eNOS and Na+/K+-ATPase. (c) 2011 Elsevier B.V. All rights reserved.
Keywords:
Insulin / Estradiol / Protein kinase B / Extracellular signal-regulated MAP kinase / Nitric oxide synthase type III / Sodium / Potassium ATPaseSource:
European Journal of Pharmacology, 2011, 655, 1-3, 23-30Funding / projects:
- Role of steroid hormones in neuroendocrine adaptation to stress and pathophysiology of metabolic syndrome - molecular mechanisms and clinical implications (RS-41009)
- Hormonal regulation of expression and activity of the nitric oxide synthase and sodium-potassium pump in experimental models of insulin resistance, diabetes and cardiovascular disorders (RS-173033)
DOI: 10.1016/j.ejphar.2011.01.016
ISSN: 0014-2999
PubMed: 21272573
WoS: 000288841000004
Scopus: 2-s2.0-79952535037
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VinčaTY - JOUR AU - Korićanac, Goran AU - Tepavčević, Snežana AU - Žakula, Zorica AU - Milosavljević, Tijana AU - Stojiljković, Mojca D. AU - Isenović, Esma R. PY - 2011 UR - https://vinar.vin.bg.ac.rs/handle/123456789/4254 AB - Insulin and estradiol share some of signaling pathways and regulate same target molecules exerting mostly beneficial cardiac effects. in order to study their cardiac interaction, ovariectomized female rats were treated with hormones, separately or simultaneously (20,30 or 40 min before analysis), and the phosphorylations of protein kinase B (Akt), extracellular signal-regulated kinases 1 and 2 (ERK 1/2), endothelial nitric oxide synthase (eNOS) were analyzed, as well as the plasma membrane content of alpha 2 subunit of Na+/K+-ATPase. Insulin, particularly, and estradiol stimulate Ser(473) Akt phosphorylation. The combined treatment was stimulatory, but less than insulin alone was. The general increase of Thr(308) Akt phosphorylation by insulin was stronger than at Ser(473) and reduced in the presence of estradiol, which also stimulated this phosphorylation given alone. The estradiol induction of ERK 1/2 phosphorylation was inverted to the decrease by the combined treatment, while insulin had no effect. Only insulin increased the plasma membrane content of alpha 2. Estradiol did increase the phosphorylation of eNOS, whereas the insulin effect was controversial. The effect of the combined treatment on target molecules was generally opposite to single hormone treatment. In summary, both hormones exerted an effect on Akt phosphorylation, but only estradiol stimulated ERK 1/2 phosphorylation. The alpha 2 plasma membrane content was increased only by insulin, while estradiol increased eNOS phosphorylation more consistently. Finally, if these hormones were administered together, it seems that they disturb each other in having a full effect on cardiac Akt, ERK 1/2, and downstream effectors, eNOS and Na+/K+-ATPase. (c) 2011 Elsevier B.V. All rights reserved. T2 - European Journal of Pharmacology T1 - Interference between insulin and estradiol signaling pathways in the regulation of cardiac eNOS and Na+/K+-ATPase VL - 655 IS - 1-3 SP - 23 EP - 30 DO - 10.1016/j.ejphar.2011.01.016 ER -
@article{ author = "Korićanac, Goran and Tepavčević, Snežana and Žakula, Zorica and Milosavljević, Tijana and Stojiljković, Mojca D. and Isenović, Esma R.", year = "2011", abstract = "Insulin and estradiol share some of signaling pathways and regulate same target molecules exerting mostly beneficial cardiac effects. in order to study their cardiac interaction, ovariectomized female rats were treated with hormones, separately or simultaneously (20,30 or 40 min before analysis), and the phosphorylations of protein kinase B (Akt), extracellular signal-regulated kinases 1 and 2 (ERK 1/2), endothelial nitric oxide synthase (eNOS) were analyzed, as well as the plasma membrane content of alpha 2 subunit of Na+/K+-ATPase. Insulin, particularly, and estradiol stimulate Ser(473) Akt phosphorylation. The combined treatment was stimulatory, but less than insulin alone was. The general increase of Thr(308) Akt phosphorylation by insulin was stronger than at Ser(473) and reduced in the presence of estradiol, which also stimulated this phosphorylation given alone. The estradiol induction of ERK 1/2 phosphorylation was inverted to the decrease by the combined treatment, while insulin had no effect. Only insulin increased the plasma membrane content of alpha 2. Estradiol did increase the phosphorylation of eNOS, whereas the insulin effect was controversial. The effect of the combined treatment on target molecules was generally opposite to single hormone treatment. In summary, both hormones exerted an effect on Akt phosphorylation, but only estradiol stimulated ERK 1/2 phosphorylation. The alpha 2 plasma membrane content was increased only by insulin, while estradiol increased eNOS phosphorylation more consistently. Finally, if these hormones were administered together, it seems that they disturb each other in having a full effect on cardiac Akt, ERK 1/2, and downstream effectors, eNOS and Na+/K+-ATPase. (c) 2011 Elsevier B.V. All rights reserved.", journal = "European Journal of Pharmacology", title = "Interference between insulin and estradiol signaling pathways in the regulation of cardiac eNOS and Na+/K+-ATPase", volume = "655", number = "1-3", pages = "23-30", doi = "10.1016/j.ejphar.2011.01.016" }
Korićanac, G., Tepavčević, S., Žakula, Z., Milosavljević, T., Stojiljković, M. D.,& Isenović, E. R.. (2011). Interference between insulin and estradiol signaling pathways in the regulation of cardiac eNOS and Na+/K+-ATPase. in European Journal of Pharmacology, 655(1-3), 23-30. https://doi.org/10.1016/j.ejphar.2011.01.016
Korićanac G, Tepavčević S, Žakula Z, Milosavljević T, Stojiljković MD, Isenović ER. Interference between insulin and estradiol signaling pathways in the regulation of cardiac eNOS and Na+/K+-ATPase. in European Journal of Pharmacology. 2011;655(1-3):23-30. doi:10.1016/j.ejphar.2011.01.016 .
Korićanac, Goran, Tepavčević, Snežana, Žakula, Zorica, Milosavljević, Tijana, Stojiljković, Mojca D., Isenović, Esma R., "Interference between insulin and estradiol signaling pathways in the regulation of cardiac eNOS and Na+/K+-ATPase" in European Journal of Pharmacology, 655, no. 1-3 (2011):23-30, https://doi.org/10.1016/j.ejphar.2011.01.016 . .