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Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes

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2009
Cytogenetic_effects_of_pub_2009.pdf (792.2Kb)
Authors
Bajić, Vladan P.
Stanimirovic, Z.
Stevanovic, J.
Spremo-Potparević, Biljana
Zivkovic, L.
Milićević, Zorka T.
Article (Published version)
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Abstract
Purpose: To assess the cytogenetic effects in vitro and in vivo of a non-cytotoxic antitumor agent with biomodulator activity, 8-chloro-3, 5 cyclic adenosine monophosphate (8-Cl-cAMP). Materials and methods: Cytogenetic effects of 8-Cl-cAMP where evaluated using the in vitro chromosome cytogenetic assail (CA) on human peripheral blood lymphocytes of healthy individuals and by hone marrow micronucleus assay in adult BALB/c mice. Results: In the in vitro chromosome CA, 8-Cl-cAMP (in all respective doses; 1.5 and 15 pin) induced mitotic inhibition and premature centromere separation (PCS) but no chromosomal damage in cultured human peripheral blood lymphocytes. In the in vivo test, single intraperitoneal (i.p) injection of 8-Cl-cAMP in doses of 10, 80 and 15 0 mg/kg showed a dose-related effect on the frequency of micronuclei, detected in murine polychromatic erythrocytes (PCE). Conclusion: The results of the present study show that genotoxicity of 8-Cl-cAMP has a different matrix of resp...onse when comparing results in vitro and in vivo, suggesting that high metabolic activity in vivo is responsible for the clastogenic potential of 8-Cl-cAMP These comparative results indicate a need of having an available battery of genotoxic tests in order to evaluate possible cytogenetic effects of novel antitumor agents.

Keywords:
8-Cl-cAMP / antitumor agents / micronuclei / premature centromere separation
Source:
Journal of BUON, 2009, 14, 1, 71-77
Projects:
  • Evaluacija dejstva hormona i citostatika prmenom citogenetičkih analiza i Komet testa (RS-143018)
  • Ecophysiological and genetic investigations of domestic animals and bees for the purpose of increasing reproductive traits and disease resistance (RS-143022)

ISSN: 1107-0625

PubMed: 19373950

WoS: 000264939400010

Scopus: 2-s2.0-64249170873
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URI
http://vinar.vin.bg.ac.rs/handle/123456789/3670
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