Show simple item record

dc.creatorPopović, Nataša M.
dc.creatorNiciforovic, Ana
dc.creatorAdžić, Miroslav
dc.creatorRadoičić, Marija B.
dc.creatorDemonacos, Constantinos
dc.creatorKrstic-Demonacos, Marija
dc.date.accessioned2018-03-01T20:44:43Z
dc.date.available2018-03-01T20:44:43Z
dc.date.issued2009
dc.identifier.issn0352-5139
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/3667
dc.description.abstractThe glucocorticoid receptor (GR) protein is a cytosolic ligand-dependent transcription factor with numerous functions regulated by post-translational modifications, including phosphorylation/dephosphorylation. Among the functions most extensively affected by GR phosphorylation are the modulation of its transcriptional activity, alterations in its interaction pattern with cofactors, nuclear translocation and selective gene transactivation. Intensive analysis of the intracellular distribution of GR phosphoisoforms and their interaction with proteins of other cellular signalling networks required the use of [gamma-(32)P]ATP as a phosphate donor, and special laboratory protection measures to avoid external irradiation and contamination. In the present study, simple and easy-to-use non-radioactive protein mobility shift assays (NMS assays) were developed using one- and/or two-dimensional gel electrophoresis based on differences in the pI and molecular mass of GR phosphoisoforms. The GR isoforms were immunodetected with specific monoclonal or polyclonal anti-GR antibodies by Western blot in three diverse systems, namely yeast BJ2168 cells expressing wild-type rat GR, rat hepatoma GRH2 cells grown in culture and brain tissue from Wistar rat experimental animals. The results obtained using the NMS assay were similar to previous results obtained with the [gamma-(32)P] ATP standard assay.en
dc.relationMinistry of Science and Technological Development of the Republic of Serbia [03E24, 143042B]
dc.rightsopenAccessen
dc.sourceJournal of the Serbian Chemical Societyen
dc.subjectglucocorticoid receptoren
dc.subjectphosphoisoformsen
dc.subjectelectrophoretic assayen
dc.titleWestern blot analysis of glucocorticoid receptor phosphoisoforms by one- and two-dimensional electrophoretic assaysen
dc.typearticleen
dcterms.abstractНицифоровиц, Aна; Крстиц-Демонацос, Марија; Поповић Наташа; Aджић Мирослав; Радоичић Марија; Демонацос, Цонстантинос;
dc.citation.volume74
dc.citation.issue3
dc.citation.spage237
dc.citation.epage244
dc.identifier.wos000264850000002
dc.identifier.doi10.2298/JSC0903237P
dc.citation.rankM23
dc.identifier.scopus2-s2.0-68949133250
dc.identifier.fulltexthttp://vinar.vin.bg.ac.rs//bitstream/id/12523/3663.pdf


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record