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Rosiglitazone modulates insulin-induced plasma membrane area changes in single 3T3-L1 adipocytes
dc.creator | Velebit, Jelena | |
dc.creator | Kovacic, Petra Brina | |
dc.creator | Prebil, Mateja | |
dc.creator | Chowdhury, Helena H. | |
dc.creator | Grilc, Sonja | |
dc.creator | Kreft, Marko | |
dc.creator | Jensen, Jorgen | |
dc.creator | Isenović, Esma R. | |
dc.creator | Zorec, Robert | |
dc.date.accessioned | 2018-03-01T20:33:29Z | |
dc.date.available | 2018-03-01T20:33:29Z | |
dc.date.issued | 2008 | |
dc.identifier.issn | 0022-2631 | |
dc.identifier.uri | https://vinar.vin.bg.ac.rs/handle/123456789/3537 | |
dc.description.abstract | In this study we hypothesized that rosiglitazone, an antidiabetic high-affinity agonist for the peroxisome proliferator-activated receptor gamma, affects the plasma membrane (PM) turnover in single 3T3-L1 adipocytes. To study the PM turnover, the patch-clamp electrophysiological method was used to measure changes in membrane capacitance (C-m), a parameter linearly related to the PM area. Microscopy results show that the presence of rosiglitazone in the differentiating medium significantly increased the differentiation of 3T3-L1 adipocytes in cell culture, based on oil red O-stained area (11.4 +/- 1.2%) vs. controls (3.1 +/- 0.5%). Moreover, rosiglitazone treatment significantly reduced the size of single 3T3-L1 adipocytes; their average radius of 21.1 +/- 1.1 mu m in controls was reduced to 17.5 +/- 0.5 mu m in rosiglitazone-treated cells. Consistent with this, insulin application increased the rate of C-m increase to 2.34 +/- 0.10%/min, which was significantly different from controls (0.12 +/- 0.08%/min). However, pretreatment of cells with rosiglitazone prior to the treatment with insulin resulted in an attenuated rate of C (m) increase. These data support the involvement of insulin in the modulation of membrane area and show that treatment by rosiglitazone reduced the insulin-mediated membrane area increase in 3T3-L1 adipocytes. | en |
dc.relation | Ministry of Higher Education, Science and Technology of the Republic of Slovenia [P3 310 381], Ministry of Science and Environmental Protection, Republic of Serbia [143030B], Republic of Slovenia and the Republic of Serbia [BI-CS/06-07-006], COST B17 | |
dc.rights | restrictedAccess | en |
dc.source | Journal of Membrane Biology | en |
dc.subject | 3T3-L1 adipocyte | en |
dc.subject | insulin | en |
dc.subject | rosiglitazone | en |
dc.subject | exocytosis | en |
dc.subject | endocytosis | en |
dc.subject | membrane area regulation | en |
dc.title | Rosiglitazone modulates insulin-induced plasma membrane area changes in single 3T3-L1 adipocytes | en |
dc.type | article | en |
dcterms.abstract | Цхоwдхурy, Хелена Х.; Исеновић Есма; Велебит, Јелена; Ковациц, Петра Брина; Пребил, Матеја; Грилц, Соња; Крефт, Марко; Јенсен, Јорген; Зорец, Роберт; | |
dc.citation.volume | 223 | |
dc.citation.issue | 3 | |
dc.citation.spage | 141 | |
dc.citation.epage | 149 | |
dc.identifier.wos | 000259733600003 | |
dc.identifier.doi | 10.1007/s00232-008-9120-x | |
dc.citation.rank | M22 | |
dc.identifier.pmid | 18754069 | |
dc.identifier.scopus | 2-s2.0-53849137995 |
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