Liver glucocorticoid receptor and heat shock protein 70 levels in rats exposed to different stress models
Апстракт
The aim of the present study was to define the stress-induced pattern of cytosolic glucocorticoid receptor (GR) and Hsp70 protein in the liver of male Wistar rats exposed to different stress models: acute (2 h/day) immobilization or cold (4 degrees C); chronic (21 days) isolation, crowding, swimming or isolation plus swimming and combined (chronic plus acute stress). Changes in plasma levels of corticosterone were studied by radioimmunoassay (RIA). The results obtained by Western immunoblotting showed that both acute stressors led to a significant decrease in cytosolic GR and Hsp70 levels. Compared to acute stress effects, only a weak decrease in the levels of GR and Hsp70 was demonstrated in chronic stress models. Chronically stressed rats, which were subsequently exposed to novel acute stressors (immobilization or cold), showed a lower extent of GR down-regulation when compared to acute stress. The exception was swimming, which partially restores this down-regulation. The observed ch...anges in the levels of these major stress-related cellular proteins in liver cytosol lead to the conclusion that chronic stressors compromise intracellular GR down-regulation in the liver.
Кључне речи:
rat / liver / stress / glucocorticoid receptor / heat shock protein 70Извор:
Physiological Research, 2008, 57, 2, 205-213Колекције
Институција/група
VinčaTY - JOUR AU - Filipović, Dragana AU - Gavrilović, Ljubica AU - Dronjak, Slađana AU - Demajo, Miroslav AU - Radojčić, Marija PY - 2008 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3477 AB - The aim of the present study was to define the stress-induced pattern of cytosolic glucocorticoid receptor (GR) and Hsp70 protein in the liver of male Wistar rats exposed to different stress models: acute (2 h/day) immobilization or cold (4 degrees C); chronic (21 days) isolation, crowding, swimming or isolation plus swimming and combined (chronic plus acute stress). Changes in plasma levels of corticosterone were studied by radioimmunoassay (RIA). The results obtained by Western immunoblotting showed that both acute stressors led to a significant decrease in cytosolic GR and Hsp70 levels. Compared to acute stress effects, only a weak decrease in the levels of GR and Hsp70 was demonstrated in chronic stress models. Chronically stressed rats, which were subsequently exposed to novel acute stressors (immobilization or cold), showed a lower extent of GR down-regulation when compared to acute stress. The exception was swimming, which partially restores this down-regulation. The observed changes in the levels of these major stress-related cellular proteins in liver cytosol lead to the conclusion that chronic stressors compromise intracellular GR down-regulation in the liver. T2 - Physiological Research T1 - Liver glucocorticoid receptor and heat shock protein 70 levels in rats exposed to different stress models VL - 57 IS - 2 SP - 205 EP - 213 UR - https://hdl.handle.net/21.15107/rcub_vinar_3477 ER -
@article{ author = "Filipović, Dragana and Gavrilović, Ljubica and Dronjak, Slađana and Demajo, Miroslav and Radojčić, Marija", year = "2008", abstract = "The aim of the present study was to define the stress-induced pattern of cytosolic glucocorticoid receptor (GR) and Hsp70 protein in the liver of male Wistar rats exposed to different stress models: acute (2 h/day) immobilization or cold (4 degrees C); chronic (21 days) isolation, crowding, swimming or isolation plus swimming and combined (chronic plus acute stress). Changes in plasma levels of corticosterone were studied by radioimmunoassay (RIA). The results obtained by Western immunoblotting showed that both acute stressors led to a significant decrease in cytosolic GR and Hsp70 levels. Compared to acute stress effects, only a weak decrease in the levels of GR and Hsp70 was demonstrated in chronic stress models. Chronically stressed rats, which were subsequently exposed to novel acute stressors (immobilization or cold), showed a lower extent of GR down-regulation when compared to acute stress. The exception was swimming, which partially restores this down-regulation. The observed changes in the levels of these major stress-related cellular proteins in liver cytosol lead to the conclusion that chronic stressors compromise intracellular GR down-regulation in the liver.", journal = "Physiological Research", title = "Liver glucocorticoid receptor and heat shock protein 70 levels in rats exposed to different stress models", volume = "57", number = "2", pages = "205-213", url = "https://hdl.handle.net/21.15107/rcub_vinar_3477" }
Filipović, D., Gavrilović, L., Dronjak, S., Demajo, M.,& Radojčić, M.. (2008). Liver glucocorticoid receptor and heat shock protein 70 levels in rats exposed to different stress models. in Physiological Research, 57(2), 205-213. https://hdl.handle.net/21.15107/rcub_vinar_3477
Filipović D, Gavrilović L, Dronjak S, Demajo M, Radojčić M. Liver glucocorticoid receptor and heat shock protein 70 levels in rats exposed to different stress models. in Physiological Research. 2008;57(2):205-213. https://hdl.handle.net/21.15107/rcub_vinar_3477 .
Filipović, Dragana, Gavrilović, Ljubica, Dronjak, Slađana, Demajo, Miroslav, Radojčić, Marija, "Liver glucocorticoid receptor and heat shock protein 70 levels in rats exposed to different stress models" in Physiological Research, 57, no. 2 (2008):205-213, https://hdl.handle.net/21.15107/rcub_vinar_3477 .