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dc.creatorIsenović, Esma R.
dc.creatorŽakula, Zorica
dc.creatorKorićanac, Goran
dc.creatorRibarac-Stepić, Nevena B.
dc.identifier.issn0862-8408 (print)
dc.description.abstractThis investigation addresses the interaction of insulin (INS) and glucocorticoid (GC) signaling in the hepatic regulation of tryptophan oxygenase (TO) enzyme activity in the rat. Male Wistar rats (200-250 g b.w) received an injection of the different doses of INS (10, 25, 50, 70 and 100 pg/200 g b.w., i.p.) and were used for experiments 3 h and 18 h after INS administration. This study shows that maximum of TO activity was found at dose of 50 mu g of INS with peak increases observed at 3 h and 18 h after injection of INS, while INS had no effect on TO activity in adrenalectomized rats. The analysis of INS effects on glucocorticoid receptor-complex (GC/GR complex) stability shows that complexes from INS-treated rats are less stable than those from control animals. In addition, INS-stimulated stability of glucocorticoid receptor (GR) protein was significantly increased from the controls. Furthermore, the results show that GC/GR complexes from INS-treated rats could be activated and accumulated at higher rate in cell nuclei of control animals. These data support the involvement of INS in modulation of GC signaling pathway which mediates, in part, the activity of TO.en
dc.sourcePhysiological Researchen
dc.subjectglucocorticoid receptoren
dc.subjectstability of glucocorticoid-receptor complexen
dc.subjecttryptophan oxygenaseen
dc.titleComparative analysis of tryptophan oxygenase activity and glucocorticoid receptor under the influence of insulinen
dcterms.abstractРибарац-Степиц, Н.; Закула, З.; Исеновић Есма; Корићанац Горан;

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