Comparative analysis of tryptophan oxygenase activity and glucocorticoid receptor under the influence of insulin
Апстракт
This investigation addresses the interaction of insulin (INS) and glucocorticoid (GC) signaling in the hepatic regulation of tryptophan oxygenase (TO) enzyme activity in the rat. Male Wistar rats (200-250 g b.w) received an injection of the different doses of INS (10, 25, 50, 70 and 100 pg/200 g b.w., i.p.) and were used for experiments 3 h and 18 h after INS administration. This study shows that maximum of TO activity was found at dose of 50 mu g of INS with peak increases observed at 3 h and 18 h after injection of INS, while INS had no effect on TO activity in adrenalectomized rats. The analysis of INS effects on glucocorticoid receptor-complex (GC/GR complex) stability shows that complexes from INS-treated rats are less stable than those from control animals. In addition, INS-stimulated stability of glucocorticoid receptor (GR) protein was significantly increased from the controls. Furthermore, the results show that GC/GR complexes from INS-treated rats could be activated and accum...ulated at higher rate in cell nuclei of control animals. These data support the involvement of INS in modulation of GC signaling pathway which mediates, in part, the activity of TO.
Кључне речи:
glucocorticoid / glucocorticoid receptor / stability of glucocorticoid-receptor complex / insulin / tryptophan oxygenaseИзвор:
Physiological Research, 2008, 57, 1, 101-107Колекције
Институција/група
VinčaTY - JOUR AU - Isenović, Esma R. AU - Žakula, Zorica AU - Korićanac, Goran AU - Ribarac-Stepić, Nevena B. PY - 2008 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3422 AB - This investigation addresses the interaction of insulin (INS) and glucocorticoid (GC) signaling in the hepatic regulation of tryptophan oxygenase (TO) enzyme activity in the rat. Male Wistar rats (200-250 g b.w) received an injection of the different doses of INS (10, 25, 50, 70 and 100 pg/200 g b.w., i.p.) and were used for experiments 3 h and 18 h after INS administration. This study shows that maximum of TO activity was found at dose of 50 mu g of INS with peak increases observed at 3 h and 18 h after injection of INS, while INS had no effect on TO activity in adrenalectomized rats. The analysis of INS effects on glucocorticoid receptor-complex (GC/GR complex) stability shows that complexes from INS-treated rats are less stable than those from control animals. In addition, INS-stimulated stability of glucocorticoid receptor (GR) protein was significantly increased from the controls. Furthermore, the results show that GC/GR complexes from INS-treated rats could be activated and accumulated at higher rate in cell nuclei of control animals. These data support the involvement of INS in modulation of GC signaling pathway which mediates, in part, the activity of TO. T2 - Physiological Research T1 - Comparative analysis of tryptophan oxygenase activity and glucocorticoid receptor under the influence of insulin VL - 57 IS - 1 SP - 101 EP - 107 UR - https://hdl.handle.net/21.15107/rcub_vinar_3422 ER -
@article{ author = "Isenović, Esma R. and Žakula, Zorica and Korićanac, Goran and Ribarac-Stepić, Nevena B.", year = "2008", abstract = "This investigation addresses the interaction of insulin (INS) and glucocorticoid (GC) signaling in the hepatic regulation of tryptophan oxygenase (TO) enzyme activity in the rat. Male Wistar rats (200-250 g b.w) received an injection of the different doses of INS (10, 25, 50, 70 and 100 pg/200 g b.w., i.p.) and were used for experiments 3 h and 18 h after INS administration. This study shows that maximum of TO activity was found at dose of 50 mu g of INS with peak increases observed at 3 h and 18 h after injection of INS, while INS had no effect on TO activity in adrenalectomized rats. The analysis of INS effects on glucocorticoid receptor-complex (GC/GR complex) stability shows that complexes from INS-treated rats are less stable than those from control animals. In addition, INS-stimulated stability of glucocorticoid receptor (GR) protein was significantly increased from the controls. Furthermore, the results show that GC/GR complexes from INS-treated rats could be activated and accumulated at higher rate in cell nuclei of control animals. These data support the involvement of INS in modulation of GC signaling pathway which mediates, in part, the activity of TO.", journal = "Physiological Research", title = "Comparative analysis of tryptophan oxygenase activity and glucocorticoid receptor under the influence of insulin", volume = "57", number = "1", pages = "101-107", url = "https://hdl.handle.net/21.15107/rcub_vinar_3422" }
Isenović, E. R., Žakula, Z., Korićanac, G.,& Ribarac-Stepić, N. B.. (2008). Comparative analysis of tryptophan oxygenase activity and glucocorticoid receptor under the influence of insulin. in Physiological Research, 57(1), 101-107. https://hdl.handle.net/21.15107/rcub_vinar_3422
Isenović ER, Žakula Z, Korićanac G, Ribarac-Stepić NB. Comparative analysis of tryptophan oxygenase activity and glucocorticoid receptor under the influence of insulin. in Physiological Research. 2008;57(1):101-107. https://hdl.handle.net/21.15107/rcub_vinar_3422 .
Isenović, Esma R., Žakula, Zorica, Korićanac, Goran, Ribarac-Stepić, Nevena B., "Comparative analysis of tryptophan oxygenase activity and glucocorticoid receptor under the influence of insulin" in Physiological Research, 57, no. 1 (2008):101-107, https://hdl.handle.net/21.15107/rcub_vinar_3422 .