Antitumor effects of a natural anthracycline analog (Aloin) involve altered activity of antioxidant enzymes in HeLaS3 cells
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2007
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The anti proliferative and cytotoxic potential of the natural anthracycline aloin from Aloe vera was tested on human uterine carcinoma HeLaS3 cells. Aloin showed a pronounced antiproliferative effect at physiological concentration (IC50 =97 mu M), caused cell cycle arrest in the S phase and markedly increased HeLaS3 cell apoptosis (to 24%). In the concentration range of 20-100 mu M, its action was accompanied by remarkable changes in the activity of almost all antioxidant enzymes: MnSOD activity was increased many fold, while CuZnSOD and iNOS activities were inhibited. Moreover, inhibition of CuZnSOD was shown to occur by direct aloin interaction with the enzyme. As catalase activity was not changed, it is suggested that such conditions were responsible for antiproliferative and cytotoxic effects owing to accumulation of H2O2. Aloin alone was a more potent proapoptotic agent than a 2 Gy fractional dose of ionizing radiation or a combination of the two. Compared to other currently used ...therapeutics, aloin, due to its less undesirable side effects and antimetastatic potential, may prove to be the agent of choice on which clinical protocols for the treatment of human cervical carcinoma should rely in future.
Ključne reči:
aloin / antioxidant enzymes / NF kappa B / HeLaS3 cells / cytotoxicity / cell cycle / proliferation / ionizing radiationIzvor:
Cancer Biology and Therapy, 2007, 6, 8, 1200-1205Finansiranje / projekti:
- Serbian Ministry of Science and Environmental Protection [143042B]
DOI: 10.4161/cbt.6.8.4383
ISSN: 1538-4047
PubMed: 17726368
WoS: 000252666800021
Scopus: 2-s2.0-39749084006
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Institucija/grupa
VinčaTY - JOUR AU - Nićiforović, Ana AU - Adžić, Miroslav AU - Spasić, Snežana D. AU - Radojčić, Marija PY - 2007 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3353 AB - The anti proliferative and cytotoxic potential of the natural anthracycline aloin from Aloe vera was tested on human uterine carcinoma HeLaS3 cells. Aloin showed a pronounced antiproliferative effect at physiological concentration (IC50 =97 mu M), caused cell cycle arrest in the S phase and markedly increased HeLaS3 cell apoptosis (to 24%). In the concentration range of 20-100 mu M, its action was accompanied by remarkable changes in the activity of almost all antioxidant enzymes: MnSOD activity was increased many fold, while CuZnSOD and iNOS activities were inhibited. Moreover, inhibition of CuZnSOD was shown to occur by direct aloin interaction with the enzyme. As catalase activity was not changed, it is suggested that such conditions were responsible for antiproliferative and cytotoxic effects owing to accumulation of H2O2. Aloin alone was a more potent proapoptotic agent than a 2 Gy fractional dose of ionizing radiation or a combination of the two. Compared to other currently used therapeutics, aloin, due to its less undesirable side effects and antimetastatic potential, may prove to be the agent of choice on which clinical protocols for the treatment of human cervical carcinoma should rely in future. T2 - Cancer Biology and Therapy T1 - Antitumor effects of a natural anthracycline analog (Aloin) involve altered activity of antioxidant enzymes in HeLaS3 cells VL - 6 IS - 8 SP - 1200 EP - 1205 DO - 10.4161/cbt.6.8.4383 UR - https://hdl.handle.net/21.15107/rcub_vinar_3353 ER -
@article{ author = "Nićiforović, Ana and Adžić, Miroslav and Spasić, Snežana D. and Radojčić, Marija", year = "2007", abstract = "The anti proliferative and cytotoxic potential of the natural anthracycline aloin from Aloe vera was tested on human uterine carcinoma HeLaS3 cells. Aloin showed a pronounced antiproliferative effect at physiological concentration (IC50 =97 mu M), caused cell cycle arrest in the S phase and markedly increased HeLaS3 cell apoptosis (to 24%). In the concentration range of 20-100 mu M, its action was accompanied by remarkable changes in the activity of almost all antioxidant enzymes: MnSOD activity was increased many fold, while CuZnSOD and iNOS activities were inhibited. Moreover, inhibition of CuZnSOD was shown to occur by direct aloin interaction with the enzyme. As catalase activity was not changed, it is suggested that such conditions were responsible for antiproliferative and cytotoxic effects owing to accumulation of H2O2. Aloin alone was a more potent proapoptotic agent than a 2 Gy fractional dose of ionizing radiation or a combination of the two. Compared to other currently used therapeutics, aloin, due to its less undesirable side effects and antimetastatic potential, may prove to be the agent of choice on which clinical protocols for the treatment of human cervical carcinoma should rely in future.", journal = "Cancer Biology and Therapy", title = "Antitumor effects of a natural anthracycline analog (Aloin) involve altered activity of antioxidant enzymes in HeLaS3 cells", volume = "6", number = "8", pages = "1200-1205", doi = "10.4161/cbt.6.8.4383", url = "https://hdl.handle.net/21.15107/rcub_vinar_3353" }
Nićiforović, A., Adžić, M., Spasić, S. D.,& Radojčić, M.. (2007). Antitumor effects of a natural anthracycline analog (Aloin) involve altered activity of antioxidant enzymes in HeLaS3 cells. in Cancer Biology and Therapy, 6(8), 1200-1205. https://doi.org/10.4161/cbt.6.8.4383 https://hdl.handle.net/21.15107/rcub_vinar_3353
Nićiforović A, Adžić M, Spasić SD, Radojčić M. Antitumor effects of a natural anthracycline analog (Aloin) involve altered activity of antioxidant enzymes in HeLaS3 cells. in Cancer Biology and Therapy. 2007;6(8):1200-1205. doi:10.4161/cbt.6.8.4383 https://hdl.handle.net/21.15107/rcub_vinar_3353 .
Nićiforović, Ana, Adžić, Miroslav, Spasić, Snežana D., Radojčić, Marija, "Antitumor effects of a natural anthracycline analog (Aloin) involve altered activity of antioxidant enzymes in HeLaS3 cells" in Cancer Biology and Therapy, 6, no. 8 (2007):1200-1205, https://doi.org/10.4161/cbt.6.8.4383 ., https://hdl.handle.net/21.15107/rcub_vinar_3353 .