Lsm proteins bind and stabilize RNAs containing 5 poly(A) tracts
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Аутори
Bergman, NaomiMoraes, Karen C. M.
Anderson, John R.
Zarić, Božidarka
Kambach, Christian
Schneider, Robert J.
Wilusz, Carol J.
Wilusz, Jeffrey
Чланак у часопису
Метаподаци
Приказ свих података о документуАпстракт
Many orthopoxvirus messenger RNAs have an unusual nontemplated poly( A) tract of 5 to 40 residues at the 5 end. The precise function of this feature is unknown. Here we show that 5 poly( A) tracts are able to repress RNA decay by inhibiting 3 - to- 5 exonucleases as well as decapping of RNA substrates. UV cross- linking analysis demonstrated that the Lsm complex associates with the 5 poly( A) tract. Furthermore, recombinant Lsm1 - 7 complex specifically binds 5 poly( A) tracts 10 to 21 nucleotides in length, consistent with the length of 5 poly( A) required for stabilization. Knockdown of Lsm1 abrogates RNA stabilization by the 5 poly( A) tract. We propose that the Lsm complex simultaneously binds the 3 and 5 ends of these unusual messenger RNAs and thereby prevents 3 - to-5 decay. The implications of this phenomenon for cellular mRNA decay are discussed.
Извор:
Nature Structural and Molecular Biology, 2007, 14, 9, 824-831Финансирање / пројекти:
- NIGMS NIH HHS [GM72481]
DOI: 10.1038/nsmb1287
ISSN: 1545-9985
PubMed: 17694069
WoS: 000249276500009
Scopus: 2-s2.0-34548472407
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Институција/група
VinčaTY - JOUR AU - Bergman, Naomi AU - Moraes, Karen C. M. AU - Anderson, John R. AU - Zarić, Božidarka AU - Kambach, Christian AU - Schneider, Robert J. AU - Wilusz, Carol J. AU - Wilusz, Jeffrey PY - 2007 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3277 AB - Many orthopoxvirus messenger RNAs have an unusual nontemplated poly( A) tract of 5 to 40 residues at the 5 end. The precise function of this feature is unknown. Here we show that 5 poly( A) tracts are able to repress RNA decay by inhibiting 3 - to- 5 exonucleases as well as decapping of RNA substrates. UV cross- linking analysis demonstrated that the Lsm complex associates with the 5 poly( A) tract. Furthermore, recombinant Lsm1 - 7 complex specifically binds 5 poly( A) tracts 10 to 21 nucleotides in length, consistent with the length of 5 poly( A) required for stabilization. Knockdown of Lsm1 abrogates RNA stabilization by the 5 poly( A) tract. We propose that the Lsm complex simultaneously binds the 3 and 5 ends of these unusual messenger RNAs and thereby prevents 3 - to-5 decay. The implications of this phenomenon for cellular mRNA decay are discussed. T2 - Nature Structural and Molecular Biology T1 - Lsm proteins bind and stabilize RNAs containing 5 poly(A) tracts VL - 14 IS - 9 SP - 824 EP - 831 DO - 10.1038/nsmb1287 ER -
@article{ author = "Bergman, Naomi and Moraes, Karen C. M. and Anderson, John R. and Zarić, Božidarka and Kambach, Christian and Schneider, Robert J. and Wilusz, Carol J. and Wilusz, Jeffrey", year = "2007", abstract = "Many orthopoxvirus messenger RNAs have an unusual nontemplated poly( A) tract of 5 to 40 residues at the 5 end. The precise function of this feature is unknown. Here we show that 5 poly( A) tracts are able to repress RNA decay by inhibiting 3 - to- 5 exonucleases as well as decapping of RNA substrates. UV cross- linking analysis demonstrated that the Lsm complex associates with the 5 poly( A) tract. Furthermore, recombinant Lsm1 - 7 complex specifically binds 5 poly( A) tracts 10 to 21 nucleotides in length, consistent with the length of 5 poly( A) required for stabilization. Knockdown of Lsm1 abrogates RNA stabilization by the 5 poly( A) tract. We propose that the Lsm complex simultaneously binds the 3 and 5 ends of these unusual messenger RNAs and thereby prevents 3 - to-5 decay. The implications of this phenomenon for cellular mRNA decay are discussed.", journal = "Nature Structural and Molecular Biology", title = "Lsm proteins bind and stabilize RNAs containing 5 poly(A) tracts", volume = "14", number = "9", pages = "824-831", doi = "10.1038/nsmb1287" }
Bergman, N., Moraes, K. C. M., Anderson, J. R., Zarić, B., Kambach, C., Schneider, R. J., Wilusz, C. J.,& Wilusz, J.. (2007). Lsm proteins bind and stabilize RNAs containing 5 poly(A) tracts. in Nature Structural and Molecular Biology, 14(9), 824-831. https://doi.org/10.1038/nsmb1287
Bergman N, Moraes KCM, Anderson JR, Zarić B, Kambach C, Schneider RJ, Wilusz CJ, Wilusz J. Lsm proteins bind and stabilize RNAs containing 5 poly(A) tracts. in Nature Structural and Molecular Biology. 2007;14(9):824-831. doi:10.1038/nsmb1287 .
Bergman, Naomi, Moraes, Karen C. M., Anderson, John R., Zarić, Božidarka, Kambach, Christian, Schneider, Robert J., Wilusz, Carol J., Wilusz, Jeffrey, "Lsm proteins bind and stabilize RNAs containing 5 poly(A) tracts" in Nature Structural and Molecular Biology, 14, no. 9 (2007):824-831, https://doi.org/10.1038/nsmb1287 . .