Biological evaluation and comparison of three different procedures for labelling of amino acids tyrosine and lysine with technetium-99m
Abstract
The Tc-99m-labelling of the amino acids tyrosine (Tyr) and lysine (Lys), fundamental building blocks of proteins and peptides, as well as biological properties of the labelled compounds are investigated. Three different approaches for the labelling with Tc-99m have been investigated: direct reduction with stannous tin in the presence of the amino acids, the preformed chelate approach through polydentate chelates DTPA and GH, and the organometallic approach using [Tc-99m(CO)(3)(H2O)(3)](+) precursor. The direct labelling approach was not successful and the yield was poor. In the presence of DTPA and GH, the labelling efficiency was between 43.6 and 97.8%, depending on the amino acid and the polydentate chelate. The use of [Tc-99m(CO)(3)(H2O)(3)](+) precursor point at the formation of Tc-99m(l) co-ordinated complexes with high yield. In this approach, pH and heating influenced the yields. The results of organ distribution study for [Tc-99m(Tyr)(H2O)(CO)(3)] and [Tc-99m(Lys)(H2O)(CO)(3)] ...show accumulation in liver, kidneys and intestine., Copyright (C) 2007 John Wiley and Sons, Ltd.
Keywords:
tyrosine / lysine / Tc-99m / [Tc-99m(CO)(3) (H2O)(3)](+) / labeling / radiopharmacySource:
Journal of Labelled Compounds and Radiopharmaceuticals, 2007, 50, 3-4, 155-163
DOI: 10.1002/jlcr.1205
ISSN: 0362-4803
WoS: 000246377600001
Scopus: 2-s2.0-34248597142
Collections
Institution/Community
VinčaTY - JOUR AU - Đokić, Divna Đ. AU - Janković, Drina PY - 2007 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3192 AB - The Tc-99m-labelling of the amino acids tyrosine (Tyr) and lysine (Lys), fundamental building blocks of proteins and peptides, as well as biological properties of the labelled compounds are investigated. Three different approaches for the labelling with Tc-99m have been investigated: direct reduction with stannous tin in the presence of the amino acids, the preformed chelate approach through polydentate chelates DTPA and GH, and the organometallic approach using [Tc-99m(CO)(3)(H2O)(3)](+) precursor. The direct labelling approach was not successful and the yield was poor. In the presence of DTPA and GH, the labelling efficiency was between 43.6 and 97.8%, depending on the amino acid and the polydentate chelate. The use of [Tc-99m(CO)(3)(H2O)(3)](+) precursor point at the formation of Tc-99m(l) co-ordinated complexes with high yield. In this approach, pH and heating influenced the yields. The results of organ distribution study for [Tc-99m(Tyr)(H2O)(CO)(3)] and [Tc-99m(Lys)(H2O)(CO)(3)] show accumulation in liver, kidneys and intestine., Copyright (C) 2007 John Wiley and Sons, Ltd. T2 - Journal of Labelled Compounds and Radiopharmaceuticals T1 - Biological evaluation and comparison of three different procedures for labelling of amino acids tyrosine and lysine with technetium-99m VL - 50 IS - 3-4 SP - 155 EP - 163 DO - 10.1002/jlcr.1205 ER -
@article{ author = "Đokić, Divna Đ. and Janković, Drina", year = "2007", abstract = "The Tc-99m-labelling of the amino acids tyrosine (Tyr) and lysine (Lys), fundamental building blocks of proteins and peptides, as well as biological properties of the labelled compounds are investigated. Three different approaches for the labelling with Tc-99m have been investigated: direct reduction with stannous tin in the presence of the amino acids, the preformed chelate approach through polydentate chelates DTPA and GH, and the organometallic approach using [Tc-99m(CO)(3)(H2O)(3)](+) precursor. The direct labelling approach was not successful and the yield was poor. In the presence of DTPA and GH, the labelling efficiency was between 43.6 and 97.8%, depending on the amino acid and the polydentate chelate. The use of [Tc-99m(CO)(3)(H2O)(3)](+) precursor point at the formation of Tc-99m(l) co-ordinated complexes with high yield. In this approach, pH and heating influenced the yields. The results of organ distribution study for [Tc-99m(Tyr)(H2O)(CO)(3)] and [Tc-99m(Lys)(H2O)(CO)(3)] show accumulation in liver, kidneys and intestine., Copyright (C) 2007 John Wiley and Sons, Ltd.", journal = "Journal of Labelled Compounds and Radiopharmaceuticals", title = "Biological evaluation and comparison of three different procedures for labelling of amino acids tyrosine and lysine with technetium-99m", volume = "50", number = "3-4", pages = "155-163", doi = "10.1002/jlcr.1205" }
Đokić, D. Đ.,& Janković, D.. (2007). Biological evaluation and comparison of three different procedures for labelling of amino acids tyrosine and lysine with technetium-99m. in Journal of Labelled Compounds and Radiopharmaceuticals, 50(3-4), 155-163. https://doi.org/10.1002/jlcr.1205
Đokić DĐ, Janković D. Biological evaluation and comparison of three different procedures for labelling of amino acids tyrosine and lysine with technetium-99m. in Journal of Labelled Compounds and Radiopharmaceuticals. 2007;50(3-4):155-163. doi:10.1002/jlcr.1205 .
Đokić, Divna Đ., Janković, Drina, "Biological evaluation and comparison of three different procedures for labelling of amino acids tyrosine and lysine with technetium-99m" in Journal of Labelled Compounds and Radiopharmaceuticals, 50, no. 3-4 (2007):155-163, https://doi.org/10.1002/jlcr.1205 . .