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dc.creatorNedeljkovic, Nadezda
dc.creatorHorvat, Anica
dc.date.accessioned2018-03-01T20:01:32Z
dc.date.available2018-03-01T20:01:32Z
dc.date.issued2007
dc.identifier.issn0026-9247 (print)
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/3164
dc.description.abstractMembrane-bound ATPases, such as Na,K-ATPase and nucleotide triphospho-diphosphohydrolase (NTPDase), being one of the first targets of a toxic action are generally considered as good markers for estimating toxicity. A bioluminescence assay was applied for fast and sensitive evaluation of heavy metals effect on the rat brain synaptosomal membrane ATPase activity. The assay consists of ATP-consuming reaction catalyzed by synaptic plasma membrane ATPases coupled to the luminescent firefly luciferase reaction, which consumes residual ATP after the course of ATPase reaction. The bioluminescence ATPase assay was applied to study the effect of heavy and transitional metals (Cu2+, Pb2+, Cd2+, Hg2+) on rat brain ATPase activity after assay optimization. All metals applied inhibited synaptic membrane ATPase activity in a concentration dependent manner. The IC (50) values (Hg2+ LT Cu2+ LT Cd2+ LT Pb2+) obtained with the bioluminescence assay were highly correlated with those obtained by the spectrophotometric method. The fast bioluminescence ATPase assay with small sample and substrate requirements could be adjusted for high-throughput environmental and pharmacological screening.en
dc.rightsrestrictedAccessen
dc.sourceMonatshefte Fur Chemieen
dc.subjectbioluminescence ATPase assayen
dc.subjectheavy metal ionsen
dc.subjectrat brainen
dc.subjectsynaptic plasma membranesen
dc.titleOne-step bioluminescence ATPase assay for the evaluation of neurotoxic effects of metal ionsen
dc.typearticleen
dcterms.abstractНедељковиц, Надезда; Хорват, Aница;
dc.citation.volume138
dc.citation.issue3
dc.citation.spage253
dc.citation.epage260
dc.identifier.wos000244837100010
dc.identifier.doi10.1007/s00706-007-0595-4
dc.citation.rankM23
dc.identifier.scopus2-s2.0-33847794169


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