One-step bioluminescence ATPase assay for the evaluation of neurotoxic effects of metal ions
Апстракт
Membrane-bound ATPases, such as Na,K-ATPase and nucleotide triphospho-diphosphohydrolase (NTPDase), being one of the first targets of a toxic action are generally considered as good markers for estimating toxicity. A bioluminescence assay was applied for fast and sensitive evaluation of heavy metals effect on the rat brain synaptosomal membrane ATPase activity. The assay consists of ATP-consuming reaction catalyzed by synaptic plasma membrane ATPases coupled to the luminescent firefly luciferase reaction, which consumes residual ATP after the course of ATPase reaction. The bioluminescence ATPase assay was applied to study the effect of heavy and transitional metals (Cu2+, Pb2+, Cd2+, Hg2+) on rat brain ATPase activity after assay optimization. All metals applied inhibited synaptic membrane ATPase activity in a concentration dependent manner. The IC (50) values (Hg2+ LT Cu2+ LT Cd2+ LT Pb2+) obtained with the bioluminescence assay were highly correlated with those obtained by the spectr...ophotometric method. The fast bioluminescence ATPase assay with small sample and substrate requirements could be adjusted for high-throughput environmental and pharmacological screening.
Кључне речи:
bioluminescence ATPase assay / heavy metal ions / rat brain / synaptic plasma membranesИзвор:
Monatshefte Fur Chemie, 2007, 138, 3, 253-260
DOI: 10.1007/s00706-007-0595-4
ISSN: 0026-9247
WoS: 000244837100010
Scopus: 2-s2.0-33847794169
Колекције
Институција/група
VinčaTY - JOUR AU - Nedeljković, Nadežda AU - Horvat, Anica PY - 2007 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3164 AB - Membrane-bound ATPases, such as Na,K-ATPase and nucleotide triphospho-diphosphohydrolase (NTPDase), being one of the first targets of a toxic action are generally considered as good markers for estimating toxicity. A bioluminescence assay was applied for fast and sensitive evaluation of heavy metals effect on the rat brain synaptosomal membrane ATPase activity. The assay consists of ATP-consuming reaction catalyzed by synaptic plasma membrane ATPases coupled to the luminescent firefly luciferase reaction, which consumes residual ATP after the course of ATPase reaction. The bioluminescence ATPase assay was applied to study the effect of heavy and transitional metals (Cu2+, Pb2+, Cd2+, Hg2+) on rat brain ATPase activity after assay optimization. All metals applied inhibited synaptic membrane ATPase activity in a concentration dependent manner. The IC (50) values (Hg2+ LT Cu2+ LT Cd2+ LT Pb2+) obtained with the bioluminescence assay were highly correlated with those obtained by the spectrophotometric method. The fast bioluminescence ATPase assay with small sample and substrate requirements could be adjusted for high-throughput environmental and pharmacological screening. T2 - Monatshefte Fur Chemie T1 - One-step bioluminescence ATPase assay for the evaluation of neurotoxic effects of metal ions VL - 138 IS - 3 SP - 253 EP - 260 DO - 10.1007/s00706-007-0595-4 ER -
@article{ author = "Nedeljković, Nadežda and Horvat, Anica", year = "2007", abstract = "Membrane-bound ATPases, such as Na,K-ATPase and nucleotide triphospho-diphosphohydrolase (NTPDase), being one of the first targets of a toxic action are generally considered as good markers for estimating toxicity. A bioluminescence assay was applied for fast and sensitive evaluation of heavy metals effect on the rat brain synaptosomal membrane ATPase activity. The assay consists of ATP-consuming reaction catalyzed by synaptic plasma membrane ATPases coupled to the luminescent firefly luciferase reaction, which consumes residual ATP after the course of ATPase reaction. The bioluminescence ATPase assay was applied to study the effect of heavy and transitional metals (Cu2+, Pb2+, Cd2+, Hg2+) on rat brain ATPase activity after assay optimization. All metals applied inhibited synaptic membrane ATPase activity in a concentration dependent manner. The IC (50) values (Hg2+ LT Cu2+ LT Cd2+ LT Pb2+) obtained with the bioluminescence assay were highly correlated with those obtained by the spectrophotometric method. The fast bioluminescence ATPase assay with small sample and substrate requirements could be adjusted for high-throughput environmental and pharmacological screening.", journal = "Monatshefte Fur Chemie", title = "One-step bioluminescence ATPase assay for the evaluation of neurotoxic effects of metal ions", volume = "138", number = "3", pages = "253-260", doi = "10.1007/s00706-007-0595-4" }
Nedeljković, N.,& Horvat, A.. (2007). One-step bioluminescence ATPase assay for the evaluation of neurotoxic effects of metal ions. in Monatshefte Fur Chemie, 138(3), 253-260. https://doi.org/10.1007/s00706-007-0595-4
Nedeljković N, Horvat A. One-step bioluminescence ATPase assay for the evaluation of neurotoxic effects of metal ions. in Monatshefte Fur Chemie. 2007;138(3):253-260. doi:10.1007/s00706-007-0595-4 .
Nedeljković, Nadežda, Horvat, Anica, "One-step bioluminescence ATPase assay for the evaluation of neurotoxic effects of metal ions" in Monatshefte Fur Chemie, 138, no. 3 (2007):253-260, https://doi.org/10.1007/s00706-007-0595-4 . .