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dc.creatorTanić, Nikola
dc.creatorBrkic, G
dc.creatorDimitrijević, Bogomir B.
dc.creatorDedović-Tanić, Nasta
dc.creatorGefen, N
dc.creatorBenharroch, D
dc.creatorGopas, J
dc.date.accessioned2018-03-01T19:50:18Z
dc.date.available2018-03-01T19:50:18Z
dc.date.issued2006
dc.identifier.issn0250-7005 (print)
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/3032
dc.description.abstractBackground: Malignant melanoma resistance to chemotherapy remains a major limitation to treatment. Our aim was to identify genes associated with drug resistance, in order to better understand the molecular events underlying the drug-resistant phenotype. Materials and Methods: A human melanoma cell line and its drug-resistant variants obtained by selection with MNNG or 6-thioguanine were used. Alterations in gene expression were characterized by differential display reverse transcription-polymerase chain reaction (DDRT-PCR). Prominent mRNA fragments present in selected variants and not in the parental cells were identified and characterized by cloning and sequencing. Differential expression was confirmed by real-time RT-PCR. Results: Three functionally distinct transcriptional products were demonstrated: the chaperonin subunit TCP 1-zeta-6A (CCT6A), the hyaluronan receptor CD44 and LPPR-2, the lipid phosphate phosphatase-related protein type-2. Conclusion: Genes with altered expression were identified in drug-resistant variants. The identified molecules may provide new insights into the molecular basis for melanoma resistance to chemotherapy.en
dc.rightsrestrictedAccessen
dc.sourceAnticancer Researchen
dc.subjectmelanomaen
dc.subjectdrug resistanceen
dc.subjectdifferentially expressed genesen
dc.subjectTCP 1-zeta-6Aen
dc.subjectCD44en
dc.subjectLPPR-2en
dc.titleIdentification of differentially expressed mRNA transcripts in drug-resistant versus parental human melanoma cell linesen
dc.typearticleen
dcterms.abstractТаниц, Н; Бркиц, Г; Дедовиц-Таниц, Н; Гефен, Н; Бенхарроцх, Д; Димитријевић Богомир Б.; Гопас, Ј;
dc.citation.volume26
dc.citation.issue3A
dc.citation.spage2137
dc.citation.epage2142
dc.identifier.wos000238490700057
dc.citation.rankM23
dc.identifier.pmid16827156


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