Show simple item record

dc.creatorNedeljkovic, N
dc.creatorBanjac, A
dc.creatorHorvat, Anica
dc.creatorStojilkovic, M
dc.creatorNikezic, G
dc.date.accessioned2018-03-01T19:22:19Z
dc.date.available2018-03-01T19:22:19Z
dc.date.issued2003
dc.identifier.issn0862-8408 (print)
dc.identifier.issn1802-9973 (electronic)
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/2702
dc.description.abstractEnzymes that hydrolyze extracellular ATP, i.e. ecto-ATPase and ecto-ATP diphosphohydrolase (ATPDase), can be differentiated by ability of the latter to hydrolyze ADP and by slightly different kinetic properties of the two enzymes. Synaptic plasma membrane fractions isolated from rat hippocampus and caudate nucleus exhibit ADP-hydrolyzing activity, as revealed by the enzyme assay, and the presence of ecto-ATPase protein, as revealed by immunological identification on Western blot. These findings indicate that both enzymes are co-expressed in the synaptic membrane compartment of hippocampal and caudate nucleus neurons. Kinetic analysis was performed to determine the relative contribution of each enzyme to the total ATP-hydrolyzing activity, while an inhibition study was carried out in order to exclude the interference of other nonspecific ATPase and phosphatase activities. Based on the kinetic properties, sensitivity to inhibitors and V-ATP/V-ADP ratio of about 2, we concluded that a substantial portion of ATP-hydrolyzing activity in both synaptic membrane preparations can be ascribed to the catalytic action of ATPDase. On the other hand, the highest catalytic efficacy when ATP is the substrate and the greater abundance of ecto-ATPase protein in caudate nucleus preparation suggest that the relative contribution of ecto-ATPase to the total ATP-hydrolyzing activity in the caudate nucleus is higher than in the hippocampus.en
dc.rightsrestrictedAccessen
dc.sourcePhysiological Researchen
dc.subjectecto-ATPaseen
dc.subjectecto-ATPhydroxylaseen
dc.subjecthippocampusen
dc.subjectcaudate nucleusen
dc.subjectraten
dc.titleEcto-ATPase and ecto-ATP-diphosphohydrolase are co-localized in rat hippocampal and caudate nucleus synaptic plasma membranesen
dc.typearticleen
dcterms.abstractНедељковиц, Н; Бањац, A; Стојилковиц, М; Никезиц, Г; Хорват, Aница;
dc.citation.volume52
dc.citation.issue6
dc.citation.spage797
dc.citation.epage804
dc.identifier.wos000188318200016
dc.citation.rankM23
dc.identifier.pmid14640903


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record