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Influence of lanolin and white soft paraffin on the determination of vitamin A in fats

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Authors
Jovanovic, MS
Gutalj, M
Ivanovic, D
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Abstract
Pharmaceutical interaction of the most frequently used substances with fats: lanolin, white soft paraffin and vitamin A palmitate was studied. The mixtures were examined by TLC with different ratios of lanolin and vitamin A palmitate, and white soft paraffin and vitamin A palmitate. Vitamin A in the mixtures was determined by the UV spectrophotometric method according to BP 93. It was established that the rise of lanolin concentration resulted in an increased apparent content of Vitamin A in fats as a consequence of lanolin absorption at the wave length of maximum absorption of vitamin A. By an increase of white soft paraffin concentration, an apparent decreased in the content of vitamin A in fats was obtained. Therefore, the suggested UV spectrophotometric method for vitamin A determination has a limited application on fats with high content of lanolin and white soft paraffin.
Keywords:
UV spectrophotometry / vitamin A palmitate / anhydrous lanolin / white soft paraffin / pharmaceutical interaction
Source:
Spectroscopy Letters, 2000, 33, 2, 227-234

DOI: 10.1080/00387010009350072

ISSN: 0038-7010

WoS: 000086305900009

Scopus: 2-s2.0-0034583592
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URI
http://vinar.vin.bg.ac.rs/handle/123456789/2336
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