Effects of progesterone and estradiol benzoate on glutathione dependent antioxidant enzyme activities in the brain of female rats
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Pajović, Snežana B.Saičić, Zorica S.
Spasić, Mihajlo
Petrović, Vojislav M.
Martinović, Jelena
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The activities of glutathione dependent antioxidant enzymes were measured in subcellular fractions of whole brain homogenates prepared from ovariectomized (OVX) female rats, untreated or treated 2 h or 24 h prior to sacrifice with a single dose of 2 mg progesterone (P) or 5 mu g estradiol benzoate (EB). Glutathione peroxidase (GSH-Px) activity was not changed following systemic administration of EB, but P increased GSH-Px in the brain of OVX rats 24 h after the treatment. The activity of glutathione reductase (GR) was suppressed by EB short time, only 2 h following treatment, whereas P increased the enzyme activity 24 h after treatment. On the other hand, the activities of catalase (CAT) and glutathione-S-transferase (GST) were not changed following systemic administration of EB or P. The present work was carried out to study the involvement of ovarian steroids, especially P, in the control of GSH-Px and GR activities, and our results suggest that oxidative stress in the brain of femal...e rats may be modulated by the level of progesterone.
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brain / antioxidant enzymes / estradiol / progesteroneSource:
General Physiology and Biophysics, 1999, 18, 1, 35-44Collections
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VinčaTY - JOUR AU - Pajović, Snežana B. AU - Saičić, Zorica S. AU - Spasić, Mihajlo AU - Petrović, Vojislav M. AU - Martinović, Jelena PY - 1999 UR - https://vinar.vin.bg.ac.rs/handle/123456789/2251 AB - The activities of glutathione dependent antioxidant enzymes were measured in subcellular fractions of whole brain homogenates prepared from ovariectomized (OVX) female rats, untreated or treated 2 h or 24 h prior to sacrifice with a single dose of 2 mg progesterone (P) or 5 mu g estradiol benzoate (EB). Glutathione peroxidase (GSH-Px) activity was not changed following systemic administration of EB, but P increased GSH-Px in the brain of OVX rats 24 h after the treatment. The activity of glutathione reductase (GR) was suppressed by EB short time, only 2 h following treatment, whereas P increased the enzyme activity 24 h after treatment. On the other hand, the activities of catalase (CAT) and glutathione-S-transferase (GST) were not changed following systemic administration of EB or P. The present work was carried out to study the involvement of ovarian steroids, especially P, in the control of GSH-Px and GR activities, and our results suggest that oxidative stress in the brain of female rats may be modulated by the level of progesterone. T2 - General Physiology and Biophysics T1 - Effects of progesterone and estradiol benzoate on glutathione dependent antioxidant enzyme activities in the brain of female rats VL - 18 IS - 1 SP - 35 EP - 44 UR - https://hdl.handle.net/21.15107/rcub_vinar_2251 ER -
@article{ author = "Pajović, Snežana B. and Saičić, Zorica S. and Spasić, Mihajlo and Petrović, Vojislav M. and Martinović, Jelena", year = "1999", abstract = "The activities of glutathione dependent antioxidant enzymes were measured in subcellular fractions of whole brain homogenates prepared from ovariectomized (OVX) female rats, untreated or treated 2 h or 24 h prior to sacrifice with a single dose of 2 mg progesterone (P) or 5 mu g estradiol benzoate (EB). Glutathione peroxidase (GSH-Px) activity was not changed following systemic administration of EB, but P increased GSH-Px in the brain of OVX rats 24 h after the treatment. The activity of glutathione reductase (GR) was suppressed by EB short time, only 2 h following treatment, whereas P increased the enzyme activity 24 h after treatment. On the other hand, the activities of catalase (CAT) and glutathione-S-transferase (GST) were not changed following systemic administration of EB or P. The present work was carried out to study the involvement of ovarian steroids, especially P, in the control of GSH-Px and GR activities, and our results suggest that oxidative stress in the brain of female rats may be modulated by the level of progesterone.", journal = "General Physiology and Biophysics", title = "Effects of progesterone and estradiol benzoate on glutathione dependent antioxidant enzyme activities in the brain of female rats", volume = "18", number = "1", pages = "35-44", url = "https://hdl.handle.net/21.15107/rcub_vinar_2251" }
Pajović, S. B., Saičić, Z. S., Spasić, M., Petrović, V. M.,& Martinović, J.. (1999). Effects of progesterone and estradiol benzoate on glutathione dependent antioxidant enzyme activities in the brain of female rats. in General Physiology and Biophysics, 18(1), 35-44. https://hdl.handle.net/21.15107/rcub_vinar_2251
Pajović SB, Saičić ZS, Spasić M, Petrović VM, Martinović J. Effects of progesterone and estradiol benzoate on glutathione dependent antioxidant enzyme activities in the brain of female rats. in General Physiology and Biophysics. 1999;18(1):35-44. https://hdl.handle.net/21.15107/rcub_vinar_2251 .
Pajović, Snežana B., Saičić, Zorica S., Spasić, Mihajlo, Petrović, Vojislav M., Martinović, Jelena, "Effects of progesterone and estradiol benzoate on glutathione dependent antioxidant enzyme activities in the brain of female rats" in General Physiology and Biophysics, 18, no. 1 (1999):35-44, https://hdl.handle.net/21.15107/rcub_vinar_2251 .