Hepatitis C virus RNA testing by nested PCR in blood preparations in Yugoslavia
Samo za registrovane korisnike
1998
Autori
Alavantić, DraganGlišić, Sanja
Radovanović, Nataša
Romić, Marija
Medić, Petar
Tomović, Olivera
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Patients receiving any kind of human blood preparations are in permanent danger of any infection including hepatitis C (HCV) infection. Testing for the presence of HCV in blood preparations is one of the steps towards safe medical treatment, One of the approaches for this testing is a detection of HCV nucleic acid. In this paper we describe a simple method for isolation of HCV RNA from blood preparations and control of HCV RNA presence in 19 intravenous and intramuscular products, manufactured in the National Blood Transfusion Institute in Belgrade. RT-PCR was performed according the rules saving RNA. Primers were located in 5 conserved region. Seven out of 19 batches of gamma-globulin, albumin, anti-tetanus and anti-rabies immunoglobulin preparations were found to be HCV RNA positive. For the time being, the PCR method is too expensive for routine HCV RNA testing of hundreds of;blood donors per day. Serological screening test of blood donors and nested PCR testing for HCV RNA. in bloo...d preparations could be an efficient combination of tests in prevention of posttransfusion hepatitis C. (C) 1998 Elsevier Science Ltd. All rights reserved.
Izvor:
Transfusion Science, 1998, 19, 2, 115-117
DOI: 10.1016/S0955-3886(98)00019-8
ISSN: 0955-3886
PubMed: 10187035
WoS: 000075996600003
Scopus: 2-s2.0-0032088177
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Alavantić, Dragan AU - Glišić, Sanja AU - Radovanović, Nataša AU - Romić, Marija AU - Medić, Petar AU - Tomović, Olivera PY - 1998 UR - https://vinar.vin.bg.ac.rs/handle/123456789/2192 AB - Patients receiving any kind of human blood preparations are in permanent danger of any infection including hepatitis C (HCV) infection. Testing for the presence of HCV in blood preparations is one of the steps towards safe medical treatment, One of the approaches for this testing is a detection of HCV nucleic acid. In this paper we describe a simple method for isolation of HCV RNA from blood preparations and control of HCV RNA presence in 19 intravenous and intramuscular products, manufactured in the National Blood Transfusion Institute in Belgrade. RT-PCR was performed according the rules saving RNA. Primers were located in 5 conserved region. Seven out of 19 batches of gamma-globulin, albumin, anti-tetanus and anti-rabies immunoglobulin preparations were found to be HCV RNA positive. For the time being, the PCR method is too expensive for routine HCV RNA testing of hundreds of;blood donors per day. Serological screening test of blood donors and nested PCR testing for HCV RNA. in blood preparations could be an efficient combination of tests in prevention of posttransfusion hepatitis C. (C) 1998 Elsevier Science Ltd. All rights reserved. T2 - Transfusion Science T1 - Hepatitis C virus RNA testing by nested PCR in blood preparations in Yugoslavia VL - 19 IS - 2 SP - 115 EP - 117 DO - 10.1016/S0955-3886(98)00019-8 ER -
@article{ author = "Alavantić, Dragan and Glišić, Sanja and Radovanović, Nataša and Romić, Marija and Medić, Petar and Tomović, Olivera", year = "1998", abstract = "Patients receiving any kind of human blood preparations are in permanent danger of any infection including hepatitis C (HCV) infection. Testing for the presence of HCV in blood preparations is one of the steps towards safe medical treatment, One of the approaches for this testing is a detection of HCV nucleic acid. In this paper we describe a simple method for isolation of HCV RNA from blood preparations and control of HCV RNA presence in 19 intravenous and intramuscular products, manufactured in the National Blood Transfusion Institute in Belgrade. RT-PCR was performed according the rules saving RNA. Primers were located in 5 conserved region. Seven out of 19 batches of gamma-globulin, albumin, anti-tetanus and anti-rabies immunoglobulin preparations were found to be HCV RNA positive. For the time being, the PCR method is too expensive for routine HCV RNA testing of hundreds of;blood donors per day. Serological screening test of blood donors and nested PCR testing for HCV RNA. in blood preparations could be an efficient combination of tests in prevention of posttransfusion hepatitis C. (C) 1998 Elsevier Science Ltd. All rights reserved.", journal = "Transfusion Science", title = "Hepatitis C virus RNA testing by nested PCR in blood preparations in Yugoslavia", volume = "19", number = "2", pages = "115-117", doi = "10.1016/S0955-3886(98)00019-8" }
Alavantić, D., Glišić, S., Radovanović, N., Romić, M., Medić, P.,& Tomović, O.. (1998). Hepatitis C virus RNA testing by nested PCR in blood preparations in Yugoslavia. in Transfusion Science, 19(2), 115-117. https://doi.org/10.1016/S0955-3886(98)00019-8
Alavantić D, Glišić S, Radovanović N, Romić M, Medić P, Tomović O. Hepatitis C virus RNA testing by nested PCR in blood preparations in Yugoslavia. in Transfusion Science. 1998;19(2):115-117. doi:10.1016/S0955-3886(98)00019-8 .
Alavantić, Dragan, Glišić, Sanja, Radovanović, Nataša, Romić, Marija, Medić, Petar, Tomović, Olivera, "Hepatitis C virus RNA testing by nested PCR in blood preparations in Yugoslavia" in Transfusion Science, 19, no. 2 (1998):115-117, https://doi.org/10.1016/S0955-3886(98)00019-8 . .