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dc.creatorMihajlovski, Katarina R.
dc.creatorDavidović, Slađana Z.
dc.creatorVeljović, Đorđe N.
dc.creatorCarević, Milica B.
dc.creatorLazić, Vesna M.
dc.creatorDimitrijević-Branković, Suzana I.
dc.description.abstractThe agricultural raw industry generates large amounts of annually by-products that create disposal problems. Hitherto, there have been no reported papers about the simultaneous production of cellulase and beta-amylase from these raw materials using Paenibacillus sp. that would reduce the costs. Thus, in this paper simultaneous cellulase (CMC-ase and avicelase) and beta-amylase production using barley bran and the application of the natural isolate Paenibacillus chitinolyticus CKS1 and potential enzymes in the hydrolysis process was studied. Response surface methodology was used to obtain the maximum enzyme activity (CMC-ase 0.405 U mL(-1), avicelase 0.433 U mL(-1) and beta-amylase 1.594U mL(-1)). Scanning electron microscopy showed degradation of the lignocellulosic-starch structure of barley bran after fermentation. The CKS1 bacterial supernatant, which contains cellulases and beta-amylase, could hydrolyze cotton fibres and barley bran, respectively. The main products after enzymatic hydrolysis of cotton fibres and barley bran, glucose (0.117 (-1)(g gmat)) and maltose (0.347 (-1)(g gmat)), were quantified by high performance liquid chromatography (HPLC). The produced enzymes could be used for hydrolysis of cotton fabric and barley bran to glucose and maltose, respectively. Application of simultaneous enzymes production using an agricultural by-product is economically and environmentally accepted and moreover, valuable biotechnological products, such as glucose and maltose, were obtained in this investigation.en
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45020/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31035/RS//
dc.sourceJournal of the Serbian Chemical Societyen
dc.subjectagricultural by-producten
dc.subjectP. chitinolyticus CKS1en
dc.subjectprocess optimizationen
dc.subjecthydrolysis productsen
dc.titleEffective valorisation of barley bran for simultaneous cellulase and beta-amylase production by Paenibacillus chitinolyticus CKS1: Statistical optimization and enzymes applicationen
dcterms.abstractМихајловски, Катарина Р.; Давидовиц, Сладана З.; Вељовиц, Дорде Н.; Царевиц, Милица Б.; Димитријевиц-Бранковиц, Сузана И.; Лазић Весна;

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