Electrochemical Determination of Natural Drug Colchicine in Pharmaceuticals and Human Serum Sample and its Interaction with DNA
Abstract
Colchicine (COLC) is a natural toxic product and secondary metabolite most commonly used to treat gout. In this study, its electrochemical behavior and determination was investigated by employing modification-free boron-doped diamond electrode (BDDE). Besides, its interaction with DNA was monitored using electrochemical methods. It was found that oxidation of this compound proceeds in two steps, where first sharp and well defined oxidation peak occurs at potential of around 1.19V, and second one at around 1.37V, in Britton-Robinson buffer solution at pH7.5. Wide dynamic range from 1 to 100 mu M was obtained with a detection limit (3 sigma(intercept)/slope) of a 0.26 mu M, based on the evaluation of first oxidation peak using differential pulse voltammetry. The proposed method was also found to be suitable for monitoring interaction of this drug with DNA as important segment for medical use. Concerning the validation, the analytical procedure shows excellent selectivity and sensitivity ...toward COLC detection and after method development it was successfully used for its quantification in pharmaceutical preparation and human serum sample, with satisfactory recovery. Obviously, this approach can be promising replacement for time-consuming and expensive separation methods.
Keywords:
differential pulse voltammetry / boron doped diamond electrode / natural alkaloid / DNA / colchicineSource:
Electroanalysis, 2017, 29, 10, 2276-2281Funding / projects:
- Application of advanced oxidation processes and nanostructured oxide materials for the removal of pollutants from the environment, development and optimisation of instrumental techniques for efficiency monitoring (RS-172030)
- Strengthening of the MagBioVin Research and Innovation Team for Development of Novel Approaches for Tumour Therapy based on Nanostructured Materials (EU-621375)
- Grant Agency of the Slovak Republic [1/0489/16]
DOI: 10.1002/elan.201700233
ISSN: 1040-0397
WoS: 000412547200014
Scopus: 2-s2.0-85023162696
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Institution/Community
VinčaTY - JOUR AU - Stanković, Dalibor M. AU - Švorc, Lubomir AU - Mariano, Jose F. M. L. AU - Ortner, Astrid AU - Kalcher, Kurt PY - 2017 UR - https://vinar.vin.bg.ac.rs/handle/123456789/1771 AB - Colchicine (COLC) is a natural toxic product and secondary metabolite most commonly used to treat gout. In this study, its electrochemical behavior and determination was investigated by employing modification-free boron-doped diamond electrode (BDDE). Besides, its interaction with DNA was monitored using electrochemical methods. It was found that oxidation of this compound proceeds in two steps, where first sharp and well defined oxidation peak occurs at potential of around 1.19V, and second one at around 1.37V, in Britton-Robinson buffer solution at pH7.5. Wide dynamic range from 1 to 100 mu M was obtained with a detection limit (3 sigma(intercept)/slope) of a 0.26 mu M, based on the evaluation of first oxidation peak using differential pulse voltammetry. The proposed method was also found to be suitable for monitoring interaction of this drug with DNA as important segment for medical use. Concerning the validation, the analytical procedure shows excellent selectivity and sensitivity toward COLC detection and after method development it was successfully used for its quantification in pharmaceutical preparation and human serum sample, with satisfactory recovery. Obviously, this approach can be promising replacement for time-consuming and expensive separation methods. T2 - Electroanalysis T1 - Electrochemical Determination of Natural Drug Colchicine in Pharmaceuticals and Human Serum Sample and its Interaction with DNA VL - 29 IS - 10 SP - 2276 EP - 2281 DO - 10.1002/elan.201700233 ER -
@article{ author = "Stanković, Dalibor M. and Švorc, Lubomir and Mariano, Jose F. M. L. and Ortner, Astrid and Kalcher, Kurt", year = "2017", abstract = "Colchicine (COLC) is a natural toxic product and secondary metabolite most commonly used to treat gout. In this study, its electrochemical behavior and determination was investigated by employing modification-free boron-doped diamond electrode (BDDE). Besides, its interaction with DNA was monitored using electrochemical methods. It was found that oxidation of this compound proceeds in two steps, where first sharp and well defined oxidation peak occurs at potential of around 1.19V, and second one at around 1.37V, in Britton-Robinson buffer solution at pH7.5. Wide dynamic range from 1 to 100 mu M was obtained with a detection limit (3 sigma(intercept)/slope) of a 0.26 mu M, based on the evaluation of first oxidation peak using differential pulse voltammetry. The proposed method was also found to be suitable for monitoring interaction of this drug with DNA as important segment for medical use. Concerning the validation, the analytical procedure shows excellent selectivity and sensitivity toward COLC detection and after method development it was successfully used for its quantification in pharmaceutical preparation and human serum sample, with satisfactory recovery. Obviously, this approach can be promising replacement for time-consuming and expensive separation methods.", journal = "Electroanalysis", title = "Electrochemical Determination of Natural Drug Colchicine in Pharmaceuticals and Human Serum Sample and its Interaction with DNA", volume = "29", number = "10", pages = "2276-2281", doi = "10.1002/elan.201700233" }
Stanković, D. M., Švorc, L., Mariano, J. F. M. L., Ortner, A.,& Kalcher, K.. (2017). Electrochemical Determination of Natural Drug Colchicine in Pharmaceuticals and Human Serum Sample and its Interaction with DNA. in Electroanalysis, 29(10), 2276-2281. https://doi.org/10.1002/elan.201700233
Stanković DM, Švorc L, Mariano JFML, Ortner A, Kalcher K. Electrochemical Determination of Natural Drug Colchicine in Pharmaceuticals and Human Serum Sample and its Interaction with DNA. in Electroanalysis. 2017;29(10):2276-2281. doi:10.1002/elan.201700233 .
Stanković, Dalibor M., Švorc, Lubomir, Mariano, Jose F. M. L., Ortner, Astrid, Kalcher, Kurt, "Electrochemical Determination of Natural Drug Colchicine in Pharmaceuticals and Human Serum Sample and its Interaction with DNA" in Electroanalysis, 29, no. 10 (2017):2276-2281, https://doi.org/10.1002/elan.201700233 . .