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dc.creatorCabarkapa, Andrea
dc.creatorDekanski, Dragana
dc.creatorŽivković, Lada
dc.creatorMilanovic-Cabarkapa, Mirjana
dc.creatorBajić, Vladan P.
dc.creatorTopalović, Dijana
dc.creatorGiampieri, Francesca
dc.creatorGasparrini, Massimiliano
dc.creatorBattino, Maurizio
dc.creatorSpremo-Potparević, Biljana
dc.date.accessioned2018-03-01T17:46:36Z
dc.date.available2018-03-01T17:46:36Z
dc.date.issued2017
dc.identifier.issn0278-6915 (print)
dc.identifier.issn1873-6351 (electronic)
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/1679
dc.description.abstractThe CaNa(2)EDTA chelation therapy is often practiced with antioxidant supplementation. Dry olive leaf extract (DOLE) is natural product with antioxidant and DNA protective properties. The effects of DOLE on the levels of DNA damage were investigated ex vivo in peripheral blood lymphocytes (PBLs) of 19 workers occupationally exposed to lead (Pb), before and after CaNa(2)EDTA chelation therapy. POLE demonstrated pronounced radical scavenging activity in concentrations GT = 1 mg/mL, and showed no genotoxicity per se, in concentrations 0.125-1 mg/mL. The level of DNA damage in PBLs of workers before chelation therapy was elevated (24.21 +/- 14.26) compared to controls (6.0 +/- 3.37). The incubation of PBLs before chelation therapy with selected concentration of DOLE lead to a severe increase of DNA damage (64.03 +/- 20.96), exhibiting prooxidant rather than antioxidant effect. After the five-day CaNa2EDTA chelation regimen, DNA damage in PBLs of workers decreased (8.26 +/- 4.62) significantly compared to baseline. Treatment of PBLs with DOLE after chelation, again produced high level of damage (41.82 +/- 23.17) and the acute prooxidant effects of DOLE remained, but, DNA damage was less severe than before chelation. The DOLE exhibits prooxidant effect in presence of Pb in lymphocytes of exposed workers, and its effect is less pronounced following the removal of Pb after standard chelation therapy. (C) 2016 Elsevier Ltd. All rights reserved.en
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173034/RS//
dc.relationRepublic of Serbia [680-00-566/2013-09/02], Republic of Italy [680-00-566/2013-09/02]
dc.rightsrestrictedAccessen
dc.sourceFood and Chemical Toxicologyen
dc.subjectLead (Pb)en
dc.subjectOxidative stressen
dc.subjectDNA damageen
dc.subjectDry olive leaf extract (DOLE)en
dc.subjectChelationen
dc.titleUnexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapyen
dc.typearticleen
dcterms.abstractЦабаркапа, Aндреа; Бајић Владан П.; Баттино, Мауризио; Топаловиц, Дијана; Милановиц-Цабаркапа, Мирјана; Гаспаррини, Массимилиано; Спремо-Потпаревиц, Биљана; Зивковиц, Лада; Гиампиери, Францесца; Декански, Драгана;
dc.citation.volume106
dc.citation.spage616
dc.citation.epage623
dc.identifier.wos000407538200007
dc.identifier.doi10.1016/j.fct.2016.12.023
dc.citation.otherPart number: B
dc.identifier.pmid28011361
dc.identifier.scopus2-s2.0-85009230956


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