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dc.creatorZrnzevic, Ivana
dc.creatorStanković, Miroslava
dc.creatorJovanovic, Vesna Stankov
dc.creatorMitic, Violeta
dc.creatorDordevic, Aleksandra
dc.creatorZlatanovic, Ivana
dc.creatorStojanovic, Gordana
dc.date.accessioned2018-03-01T17:44:36Z
dc.date.available2018-03-01T17:44:36Z
dc.date.issued2017
dc.identifier.issn1611-2156 (print)
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/1656
dc.description.abstractIn the present investigation, effects of Ramalina capitata acetone extract on micronucleus distribution on human lymphocytes, on cholinesterase activity and antioxidant activity (by the CUPRAC method) were examined, for the first time as well as its HPLC profile. Additionally, total phenolic compounds (TPC), antioxidant properties (estimated via DPPH, ABTS and TRP assays) and antibacterial activity were determined. The predominant phenolic compounds in this extract were evernic, everninic and obtusatic acids. Acetone extract of R. capitata at concentration of 2 mu g mL(-1) decreased a frequency of micronuclei (MN) for 14.8 %. The extract reduces the concentration of DPPH and ABTS radicals for 21.2 and 36.1 % (respectively). Values for total reducing power (TRP) and cupric reducing capacity (CUPRAC) were 0.4624 +/- 0.1064 mu g ascorbic acid equivalents (AAE) per mg of dry extract, and 6.1176 +/- 0.2964 mu g Trolox equivalents (TE) per mg of dry extract, respectively. The total phenol content was 670.6376 +/- 66.554 mu g galic acid equivalents (GAE) per mg of dry extract. Tested extract at concentration of 2 mg mL(-1) exhibited inhibition effect (5.2 %) on pooled human serum cholinesterase. The antimicrobial assay showed that acetone extract had inhibition effect towards Gram-positive strains. The results of manifested antioxidant activity, reducing the number of micronuclei in human lymphocytes, and antibacterial activity recommends R. capitata extract for further in vivo studies.en
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172047/RS//
dc.rightsopenAccessen
dc.sourceEXCLI Journalen
dc.subjectRamalina capitata acetone extracten
dc.subjectmicronucleus testen
dc.subjectantioxidant activityen
dc.subjectcholinesterase inhibitionen
dc.subjectantimicrobial activityen
dc.subjectchemical compositionen
dc.titleRamalina Capitata (Ach.) Nyl. Acetone Extract: Hplc Analysis, Genotoxicity, Cholinesterase, Antioxidant and Antibacterial Activityen
dc.typearticleen
dcterms.abstractЗрнзевиц, Ивана; Станковиц, Мирослава; Јовановиц, Весна Станков; Митиц, Виолета; Дордевиц, Aлександра; Златановиц, Ивана; Стојановиц, Гордана;
dc.citation.volume16
dc.citation.spage679
dc.citation.epage687
dc.identifier.wos000406266500006
dc.identifier.doi10.17179/excli2017-301
dc.identifier.pmid28827984
dc.identifier.scopus2-s2.0-85019699033
dc.identifier.fulltexthttp://vinar.vin.bg.ac.rs//bitstream/id/12006/1652.pdf


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