17 beta-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats
17ß-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats
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2017
Authors
Zafirović, SonjaObradović, Milan M.
Sudar-Milovanović, Emina
Jovanović, Aleksandra
Stanimirović, Julijana
Stewart, Alan J.
Pitt, Samantha J.
Isenović, Esma R.
Article (Published version)
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The aim of this study was to investigate the in vivo effects of 17 beta-estradiol (E-2) on myocardial metabolism and inducible nitric oxide synthase (iNOS) expression/activity in obese rats. Male Wistar rats were fed with a normal or a high fat (HF) diet (42% fat) for 10 weeks. Half of the HF fed rats were treated with a single dose of E-2 while the other half were placebo-treated. 24 h after treatment animals were sacrificed. E-2 reduced cardiac free fatty acid (FFA) (p LT 0.05), L-arginine (p LT 0.01), iNOS mRNA (p LT 0.01), and protein (p LT 0.05) levels and translocation of the FFA transporter (CD36) (p LT 0.01) to the plasma membrane (PM) in HF fed rats. In contrast, Akt phosphorylation at Thr308 (p LT 0.05) and translocation of the glucose transporter GLUT4 (p LT 0.05) to the PM increased after E-2 treatment in HF rats. Our results indicate that E-2 acts via the PI3K/Akt signalling pathway to partially protect myocardial metabolism by attenuating the detrimental effects of increa...sed iNOS expression/activity in HF fed rats. (C) 2017 Elsevier B.V. All rights reserved.
Keywords:
Estradiol / Obesity / Myocardial metabolism / Cardiovascular diseases / Inducible nitric oxide synthaseSource:
Molecular and Cellular Endocrinology, 2017, 446, C, 12-20Funding / projects:
- Hormonal regulation of expression and activity of the nitric oxide synthase and sodium-potassium pump in experimental models of insulin resistance, diabetes and cardiovascular disorders (RS-MESTD-Basic Research (BR or ON)-173033)
DOI: 10.1016/j.mce.2017.02.001
ISSN: 0303-7207
PubMed: 28163099
WoS: 000399509600002
Scopus: 2-s2.0-85012867945
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VinčaTY - JOUR AU - Zafirović, Sonja AU - Obradović, Milan M. AU - Sudar-Milovanović, Emina AU - Jovanović, Aleksandra AU - Stanimirović, Julijana AU - Stewart, Alan J. AU - Pitt, Samantha J. AU - Isenović, Esma R. PY - 2017 UR - https://vinar.vin.bg.ac.rs/handle/123456789/1520 AB - The aim of this study was to investigate the in vivo effects of 17 beta-estradiol (E-2) on myocardial metabolism and inducible nitric oxide synthase (iNOS) expression/activity in obese rats. Male Wistar rats were fed with a normal or a high fat (HF) diet (42% fat) for 10 weeks. Half of the HF fed rats were treated with a single dose of E-2 while the other half were placebo-treated. 24 h after treatment animals were sacrificed. E-2 reduced cardiac free fatty acid (FFA) (p LT 0.05), L-arginine (p LT 0.01), iNOS mRNA (p LT 0.01), and protein (p LT 0.05) levels and translocation of the FFA transporter (CD36) (p LT 0.01) to the plasma membrane (PM) in HF fed rats. In contrast, Akt phosphorylation at Thr308 (p LT 0.05) and translocation of the glucose transporter GLUT4 (p LT 0.05) to the PM increased after E-2 treatment in HF rats. Our results indicate that E-2 acts via the PI3K/Akt signalling pathway to partially protect myocardial metabolism by attenuating the detrimental effects of increased iNOS expression/activity in HF fed rats. (C) 2017 Elsevier B.V. All rights reserved. T2 - Molecular and Cellular Endocrinology T1 - 17 beta-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats VL - 446 IS - C SP - 12 EP - 20 DO - 10.1016/j.mce.2017.02.001 ER -
@article{ author = "Zafirović, Sonja and Obradović, Milan M. and Sudar-Milovanović, Emina and Jovanović, Aleksandra and Stanimirović, Julijana and Stewart, Alan J. and Pitt, Samantha J. and Isenović, Esma R.", year = "2017", abstract = "The aim of this study was to investigate the in vivo effects of 17 beta-estradiol (E-2) on myocardial metabolism and inducible nitric oxide synthase (iNOS) expression/activity in obese rats. Male Wistar rats were fed with a normal or a high fat (HF) diet (42% fat) for 10 weeks. Half of the HF fed rats were treated with a single dose of E-2 while the other half were placebo-treated. 24 h after treatment animals were sacrificed. E-2 reduced cardiac free fatty acid (FFA) (p LT 0.05), L-arginine (p LT 0.01), iNOS mRNA (p LT 0.01), and protein (p LT 0.05) levels and translocation of the FFA transporter (CD36) (p LT 0.01) to the plasma membrane (PM) in HF fed rats. In contrast, Akt phosphorylation at Thr308 (p LT 0.05) and translocation of the glucose transporter GLUT4 (p LT 0.05) to the PM increased after E-2 treatment in HF rats. Our results indicate that E-2 acts via the PI3K/Akt signalling pathway to partially protect myocardial metabolism by attenuating the detrimental effects of increased iNOS expression/activity in HF fed rats. (C) 2017 Elsevier B.V. All rights reserved.", journal = "Molecular and Cellular Endocrinology", title = "17 beta-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats", volume = "446", number = "C", pages = "12-20", doi = "10.1016/j.mce.2017.02.001" }
Zafirović, S., Obradović, M. M., Sudar-Milovanović, E., Jovanović, A., Stanimirović, J., Stewart, A. J., Pitt, S. J.,& Isenović, E. R.. (2017). 17 beta-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats. in Molecular and Cellular Endocrinology, 446(C), 12-20. https://doi.org/10.1016/j.mce.2017.02.001
Zafirović S, Obradović MM, Sudar-Milovanović E, Jovanović A, Stanimirović J, Stewart AJ, Pitt SJ, Isenović ER. 17 beta-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats. in Molecular and Cellular Endocrinology. 2017;446(C):12-20. doi:10.1016/j.mce.2017.02.001 .
Zafirović, Sonja, Obradović, Milan M., Sudar-Milovanović, Emina, Jovanović, Aleksandra, Stanimirović, Julijana, Stewart, Alan J., Pitt, Samantha J., Isenović, Esma R., "17 beta-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats" in Molecular and Cellular Endocrinology, 446, no. C (2017):12-20, https://doi.org/10.1016/j.mce.2017.02.001 . .