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dc.creatorBondžić, Aleksandra
dc.creatorJanjić, Goran V.
dc.creatorDramićanin, Miroslav
dc.creatorMessori, Luigi
dc.creatorMassai, Lara
dc.creatorParac Vogt, Tatjana N.
dc.creatorVasić, Vesna M.
dc.date.accessioned2018-03-01T17:28:58Z
dc.date.available2018-03-01T17:28:58Z
dc.date.issued2017
dc.identifier.issn1756-5901
dc.identifier.issn1756-591X
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/1476
dc.description.abstractNa/K-ATPase is emerging as an important target for a variety of anticancer metal-based drugs. The interactions of Na/K-ATPase (in its E1 state) with three representative and structurally related cytotoxic gold(III) complexes, i.e. [Au(bipy)(OH)(2)][PF6], bipy = 2,2-bipyridine; [Au(py(dmb)-H)(CH3COO)(2)], py(dmb)-H = deprotonated 6-(1,1-dimethylbenzyl)-pyridine and [Au(bipy(dmb)-H)(OH)][PF6], bipy(c)-H = deprotonated 6-(1,1-dimethylbenzyl)-2,20-bipyridine, are investigated here in depth using a variety of spectroscopic methods, in combination with docking studies. Detailed information is gained on the conformational and structural changes experienced by the enzyme upon binding of these gold(III) complexes. The quenching constants of intrinsic enzyme fluorescence, the fraction of Trp residues accessible to gold(III) complexes and the reaction stoichiometries were determined in various cases. Specific hypotheses are made concerning the binding mode of these gold(III) complexes to the enzyme and the likely binding sites. Differences in their binding behaviour toward Na/K-ATPase are explained on the ground of their distinctive structural features. The present results offer further support to the view that Na/K-ATPase may be a relevant biomolecular target for cytotoxic gold(III) compounds of medicinal interest and may thus be involved in their overall mode of action.en
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172023/RS//
dc.relationBeneficentia Stiftung (Vaduz, Liechtenstein), AIRC [IG-16049], ITT, Fondazione CRF, CIRCMSB
dc.rightsrestrictedAccessen
dc.sourceMetallomicsen
dc.titleNa/K-ATPase as a target for anticancer metal based drugs: insights into molecular interactions with selected gold(III) complexesen
dc.typearticleen
dcterms.abstractМессори, Луиги; Бонджић Aлександра; Массаи, Лара; Драмићанин Мирослав; Јањиц, Горан В.; Васић Весна М; Парац Вогт, Татјана Н.;
dc.citation.volume9
dc.citation.issue3
dc.citation.spage292
dc.citation.epage300
dc.identifier.wos000397438600009
dc.identifier.doi10.1039/c7mt00017k
dc.citation.rankM21
dc.identifier.pmid28181616
dc.identifier.scopus2-s2.0-85024877237


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