Cytotoxicity and genotoxicity of a low-shrinkage monomer and monoacylphosphine oxide photoinitiator: Comparative analyses of individual toxicity and combination effects in mixtures
Samo za registrovane korisnike
2017
Autori
Manojlović, DragicaDramićanin, Miroslav
Miletić, Vesna
Mitić-Ćulafić, Dragana
Jovanović, Bojana
Nikolić, Biljana
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Objective. To compare cytotoxicity and genotoxicity of novel urethane-based monomer FIT-852 and monoacylphosphine oxide photoinitiator (Lucirin TPO) with conventional Bisphenol A-glycidyl-methacrylate (BisGMA) and triethylene glycol dimethacrylate (TEGDMA) monomers and camphorquinone (CQ)/amine photoinitiator system, respectively. Moreover, we quantified and analyzed the combinatorial effects of individual substances in resin-based mixtures concerning the nature of the combinatorial effects. Methods. Cytotoxic and genotoxic effects of BisGMA, FIT, TEGDMA, CQ DMAEMA and TPO and their combined toxicity in four clinically relevant mixtures (FIT/TPO, FIT/CQ BisGMA/TPO, BisGMA/CQ) were tested on human fetal lung fibroblasts MRC-5 using MTT and Comet assays. We assessed combination effects of monomers and photoinitiators on overall toxicity from the measured concentration-effect relationships. Combination index (CI) was calculated on the basis of the median-effect equation derived from the m...ass-action law principle. Results. Individual substances showed decreasing cytotoxic effects in the following order: BisGMA GT TPO GT FIT GT CQ GT DMAEMA GT TEGDMA. Experimental mixtures showed decreasing cytotoxic effects in the order BisGMA/TPO GT BisGMA/CQ GT FIT/CQ GT FIT/TPO. FIT-based mixtures exhibited antagonistic cytotoxic effects between components while BisGMA-based mixtures demonstrated synergistic effects at ED50 TPO amplified both antagonistic and synergistic cytotoxic effects in mixtures. Pure substances showed genotoxicity in the following order: TPO GT BisGMA GT FIT GT CQ GT TEGDMA. We did not detect the genotoxic potential of DMAEMA. The rank of genotoxic concentrations of the mixtures was: BisGMA/TPO GT BisGMA/CQ GT FIT/CQ GT FIT/TPO. Significance. Lower cytotoxicity and genotoxicity of FIT than BisGMA suggests its greater bio-co mpatibility. Conversely, photoinitiator TPO was significantly more cytotoxic and genotoxic than both CQ and DMAEMA. CI values showed that components of FIT-based mixtures exhibit an antagonistic cytotoxic effect, while compontents of BisGMA-based mixtures show synergism.
Ključne reči:
Cytotoxicity / Genotoxicity / Combinatorial effect / Monomer / PhotoinitiatorIzvor:
Dental Materials, 2017, 33, 4, 454-466Finansiranje / projekti:
- Materijali redukovane dimenzionalnosti za efikasnu apsorpciju svetlosti i konverziju energije (RS-45020)
- Razvoj i primena metoda i materijala za monitoring novih zagađujućih i toksičnih organskih materija i teških metala (RS-172007)
DOI: 10.1016/j.dental.2017.02.002
ISSN: 0109-5641; 1879-0097
PubMed: 28258769
WoS: 000396410200014
Scopus: 2-s2.0-85014043733
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Manojlović, Dragica AU - Dramićanin, Miroslav AU - Miletić, Vesna AU - Mitić-Ćulafić, Dragana AU - Jovanović, Bojana AU - Nikolić, Biljana PY - 2017 UR - https://vinar.vin.bg.ac.rs/handle/123456789/1462 AB - Objective. To compare cytotoxicity and genotoxicity of novel urethane-based monomer FIT-852 and monoacylphosphine oxide photoinitiator (Lucirin TPO) with conventional Bisphenol A-glycidyl-methacrylate (BisGMA) and triethylene glycol dimethacrylate (TEGDMA) monomers and camphorquinone (CQ)/amine photoinitiator system, respectively. Moreover, we quantified and analyzed the combinatorial effects of individual substances in resin-based mixtures concerning the nature of the combinatorial effects. Methods. Cytotoxic and genotoxic effects of BisGMA, FIT, TEGDMA, CQ DMAEMA and TPO and their combined toxicity in four clinically relevant mixtures (FIT/TPO, FIT/CQ BisGMA/TPO, BisGMA/CQ) were tested on human fetal lung fibroblasts MRC-5 using MTT and Comet assays. We assessed combination effects of monomers and photoinitiators on overall toxicity from the measured concentration-effect relationships. Combination index (CI) was calculated on the basis of the median-effect equation derived from the mass-action law principle. Results. Individual substances showed decreasing cytotoxic effects in the following order: BisGMA GT TPO GT FIT GT CQ GT DMAEMA GT TEGDMA. Experimental mixtures showed decreasing cytotoxic effects in the order BisGMA/TPO GT BisGMA/CQ GT FIT/CQ GT FIT/TPO. FIT-based mixtures exhibited antagonistic cytotoxic effects between components while BisGMA-based mixtures demonstrated synergistic effects at ED50 TPO amplified both antagonistic and synergistic cytotoxic effects in mixtures. Pure substances showed genotoxicity in the following order: TPO GT BisGMA GT FIT GT CQ GT TEGDMA. We did not detect the genotoxic potential of DMAEMA. The rank of genotoxic concentrations of the mixtures was: BisGMA/TPO GT BisGMA/CQ GT FIT/CQ GT FIT/TPO. Significance. Lower cytotoxicity and genotoxicity of FIT than BisGMA suggests its greater bio-co mpatibility. Conversely, photoinitiator TPO was significantly more cytotoxic and genotoxic than both CQ and DMAEMA. CI values showed that components of FIT-based mixtures exhibit an antagonistic cytotoxic effect, while compontents of BisGMA-based mixtures show synergism. T2 - Dental Materials T1 - Cytotoxicity and genotoxicity of a low-shrinkage monomer and monoacylphosphine oxide photoinitiator: Comparative analyses of individual toxicity and combination effects in mixtures VL - 33 IS - 4 SP - 454 EP - 466 DO - 10.1016/j.dental.2017.02.002 ER -
@article{ author = "Manojlović, Dragica and Dramićanin, Miroslav and Miletić, Vesna and Mitić-Ćulafić, Dragana and Jovanović, Bojana and Nikolić, Biljana", year = "2017", abstract = "Objective. To compare cytotoxicity and genotoxicity of novel urethane-based monomer FIT-852 and monoacylphosphine oxide photoinitiator (Lucirin TPO) with conventional Bisphenol A-glycidyl-methacrylate (BisGMA) and triethylene glycol dimethacrylate (TEGDMA) monomers and camphorquinone (CQ)/amine photoinitiator system, respectively. Moreover, we quantified and analyzed the combinatorial effects of individual substances in resin-based mixtures concerning the nature of the combinatorial effects. Methods. Cytotoxic and genotoxic effects of BisGMA, FIT, TEGDMA, CQ DMAEMA and TPO and their combined toxicity in four clinically relevant mixtures (FIT/TPO, FIT/CQ BisGMA/TPO, BisGMA/CQ) were tested on human fetal lung fibroblasts MRC-5 using MTT and Comet assays. We assessed combination effects of monomers and photoinitiators on overall toxicity from the measured concentration-effect relationships. Combination index (CI) was calculated on the basis of the median-effect equation derived from the mass-action law principle. Results. Individual substances showed decreasing cytotoxic effects in the following order: BisGMA GT TPO GT FIT GT CQ GT DMAEMA GT TEGDMA. Experimental mixtures showed decreasing cytotoxic effects in the order BisGMA/TPO GT BisGMA/CQ GT FIT/CQ GT FIT/TPO. FIT-based mixtures exhibited antagonistic cytotoxic effects between components while BisGMA-based mixtures demonstrated synergistic effects at ED50 TPO amplified both antagonistic and synergistic cytotoxic effects in mixtures. Pure substances showed genotoxicity in the following order: TPO GT BisGMA GT FIT GT CQ GT TEGDMA. We did not detect the genotoxic potential of DMAEMA. The rank of genotoxic concentrations of the mixtures was: BisGMA/TPO GT BisGMA/CQ GT FIT/CQ GT FIT/TPO. Significance. Lower cytotoxicity and genotoxicity of FIT than BisGMA suggests its greater bio-co mpatibility. Conversely, photoinitiator TPO was significantly more cytotoxic and genotoxic than both CQ and DMAEMA. CI values showed that components of FIT-based mixtures exhibit an antagonistic cytotoxic effect, while compontents of BisGMA-based mixtures show synergism.", journal = "Dental Materials", title = "Cytotoxicity and genotoxicity of a low-shrinkage monomer and monoacylphosphine oxide photoinitiator: Comparative analyses of individual toxicity and combination effects in mixtures", volume = "33", number = "4", pages = "454-466", doi = "10.1016/j.dental.2017.02.002" }
Manojlović, D., Dramićanin, M., Miletić, V., Mitić-Ćulafić, D., Jovanović, B.,& Nikolić, B.. (2017). Cytotoxicity and genotoxicity of a low-shrinkage monomer and monoacylphosphine oxide photoinitiator: Comparative analyses of individual toxicity and combination effects in mixtures. in Dental Materials, 33(4), 454-466. https://doi.org/10.1016/j.dental.2017.02.002
Manojlović D, Dramićanin M, Miletić V, Mitić-Ćulafić D, Jovanović B, Nikolić B. Cytotoxicity and genotoxicity of a low-shrinkage monomer and monoacylphosphine oxide photoinitiator: Comparative analyses of individual toxicity and combination effects in mixtures. in Dental Materials. 2017;33(4):454-466. doi:10.1016/j.dental.2017.02.002 .
Manojlović, Dragica, Dramićanin, Miroslav, Miletić, Vesna, Mitić-Ćulafić, Dragana, Jovanović, Bojana, Nikolić, Biljana, "Cytotoxicity and genotoxicity of a low-shrinkage monomer and monoacylphosphine oxide photoinitiator: Comparative analyses of individual toxicity and combination effects in mixtures" in Dental Materials, 33, no. 4 (2017):454-466, https://doi.org/10.1016/j.dental.2017.02.002 . .