Europium-doped GdVO4 nanocrystals as a luminescent probe for hydrogen peroxide and for enzymatic sensing of glucose
Authorized Users Only
2017
Authors
Muhr, VerenaBuchner, Markus
Hirsch, Thomas
Jovanović, Dragana J.
Dolić, Slobodan D.
Dramićanin, Miroslav
Wolfbeis, Otto S.
Article (Published version)
Metadata
Show full item recordAbstract
The authors describe the preparation of Eu3+-doped GdVO4 nanocrystals (NCs) by precipitation of the Cd3+(Eu3+)-citrate complex which was then converted to the respective vanadate by dialysis. The fractions of Eu3+ ranged from 5 to 100 mol%. The NCs were characterized by XRD, TEM, ICP-OES and dynamic light scattering which revealed that they possess superior colloidal stability in aqueous solutions in that no precipitation can be observed even after several months. The NCs display red and largely red shifted fluorescence (peaking at 618 nm) on photoexcitation at around 300 nm. Fluorescence is strongly quenched by hydrogen peroxide. It is also shown that the fraction of doping with Eu3+ strongly affects quenchability. Most efficient quenching by H2O2 is observed if the NCs are doped with 50% of Eu3+. The findings were exploited to develop a fluorometric assay for H2O2 that works in the 5 to 250 mu M concentration range, with a limit of detection as low as 1.6 mu M (at a signal-to-noise r...atio of 3). The probe was further employed to design a highly sensitive enzymatic assay for glucose via measurement of the quantity of H2O2 formed as a result of the catalytic action of glucose oxidase. (C) 2016 Elsevier B.V. All rights reserved.
Keywords:
Nanoprobe / Luminescent probe / Nanomaterial / Lanthanide nanoparticle / Hydrogen peroxide / Transmission electron microscopy / DLS / Zeta potential / Quantum yield / Quenching / Stern-Volmer plot / Glucose oxidase / Enzymatic assaySource:
Sensors and Actuators B: Chemical, 2017, 241, 349-356
DOI: 10.1016/j.snb.2016.10.090
ISSN: 0925-4005
WoS: 000393253700045
Scopus: 2-s2.0-84992183830
Collections
Institution/Community
VinčaTY - JOUR AU - Muhr, Verena AU - Buchner, Markus AU - Hirsch, Thomas AU - Jovanović, Dragana J. AU - Dolić, Slobodan D. AU - Dramićanin, Miroslav AU - Wolfbeis, Otto S. PY - 2017 UR - https://vinar.vin.bg.ac.rs/handle/123456789/1416 AB - The authors describe the preparation of Eu3+-doped GdVO4 nanocrystals (NCs) by precipitation of the Cd3+(Eu3+)-citrate complex which was then converted to the respective vanadate by dialysis. The fractions of Eu3+ ranged from 5 to 100 mol%. The NCs were characterized by XRD, TEM, ICP-OES and dynamic light scattering which revealed that they possess superior colloidal stability in aqueous solutions in that no precipitation can be observed even after several months. The NCs display red and largely red shifted fluorescence (peaking at 618 nm) on photoexcitation at around 300 nm. Fluorescence is strongly quenched by hydrogen peroxide. It is also shown that the fraction of doping with Eu3+ strongly affects quenchability. Most efficient quenching by H2O2 is observed if the NCs are doped with 50% of Eu3+. The findings were exploited to develop a fluorometric assay for H2O2 that works in the 5 to 250 mu M concentration range, with a limit of detection as low as 1.6 mu M (at a signal-to-noise ratio of 3). The probe was further employed to design a highly sensitive enzymatic assay for glucose via measurement of the quantity of H2O2 formed as a result of the catalytic action of glucose oxidase. (C) 2016 Elsevier B.V. All rights reserved. T2 - Sensors and Actuators B: Chemical T1 - Europium-doped GdVO4 nanocrystals as a luminescent probe for hydrogen peroxide and for enzymatic sensing of glucose VL - 241 SP - 349 EP - 356 DO - 10.1016/j.snb.2016.10.090 ER -
@article{ author = "Muhr, Verena and Buchner, Markus and Hirsch, Thomas and Jovanović, Dragana J. and Dolić, Slobodan D. and Dramićanin, Miroslav and Wolfbeis, Otto S.", year = "2017", abstract = "The authors describe the preparation of Eu3+-doped GdVO4 nanocrystals (NCs) by precipitation of the Cd3+(Eu3+)-citrate complex which was then converted to the respective vanadate by dialysis. The fractions of Eu3+ ranged from 5 to 100 mol%. The NCs were characterized by XRD, TEM, ICP-OES and dynamic light scattering which revealed that they possess superior colloidal stability in aqueous solutions in that no precipitation can be observed even after several months. The NCs display red and largely red shifted fluorescence (peaking at 618 nm) on photoexcitation at around 300 nm. Fluorescence is strongly quenched by hydrogen peroxide. It is also shown that the fraction of doping with Eu3+ strongly affects quenchability. Most efficient quenching by H2O2 is observed if the NCs are doped with 50% of Eu3+. The findings were exploited to develop a fluorometric assay for H2O2 that works in the 5 to 250 mu M concentration range, with a limit of detection as low as 1.6 mu M (at a signal-to-noise ratio of 3). The probe was further employed to design a highly sensitive enzymatic assay for glucose via measurement of the quantity of H2O2 formed as a result of the catalytic action of glucose oxidase. (C) 2016 Elsevier B.V. All rights reserved.", journal = "Sensors and Actuators B: Chemical", title = "Europium-doped GdVO4 nanocrystals as a luminescent probe for hydrogen peroxide and for enzymatic sensing of glucose", volume = "241", pages = "349-356", doi = "10.1016/j.snb.2016.10.090" }
Muhr, V., Buchner, M., Hirsch, T., Jovanović, D. J., Dolić, S. D., Dramićanin, M.,& Wolfbeis, O. S.. (2017). Europium-doped GdVO4 nanocrystals as a luminescent probe for hydrogen peroxide and for enzymatic sensing of glucose. in Sensors and Actuators B: Chemical, 241, 349-356. https://doi.org/10.1016/j.snb.2016.10.090
Muhr V, Buchner M, Hirsch T, Jovanović DJ, Dolić SD, Dramićanin M, Wolfbeis OS. Europium-doped GdVO4 nanocrystals as a luminescent probe for hydrogen peroxide and for enzymatic sensing of glucose. in Sensors and Actuators B: Chemical. 2017;241:349-356. doi:10.1016/j.snb.2016.10.090 .
Muhr, Verena, Buchner, Markus, Hirsch, Thomas, Jovanović, Dragana J., Dolić, Slobodan D., Dramićanin, Miroslav, Wolfbeis, Otto S., "Europium-doped GdVO4 nanocrystals as a luminescent probe for hydrogen peroxide and for enzymatic sensing of glucose" in Sensors and Actuators B: Chemical, 241 (2017):349-356, https://doi.org/10.1016/j.snb.2016.10.090 . .