Inhibition of Na+/K+-ATPase and cytotoxicity of a few selected gold(III) complexes
Čolović, Mirjana B.
Cinellu, Maria Agostina
Vasić, Vesna M.
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Na+/K+-ATPase is in charge of maintaining the ionic and osmotic intracellular balance by using ATP as an energy source to drive excess Na+ ions out of the cell in exchange for K+ ions. We explored whether three representative cytotoxic gold(III) compounds might interfere with Na+/K+-ATPase and cause its inhibition at pharmacologically relevant concentrations. The tested complexes were [Au(bipy)(OH)(2)][PF6] (bipy = 2,2-bipyridine), [Au (py(dmb)-H)(CH3COO)(2)] (py(dmb)-H = deprotonated 6-(1,1-dimethylbenzyl)-pyridine), and [Au(bipy(dmb)-H)(OH)][PF6] (bipy(dmb)-H = deprotonated 6-(1,1-dimethylbenzyl)-2,2-bipyridine). We found that all of them caused a pronounced and similar inhibition of Na+/K+-ATPase activity. Inhibition was found to be non-competitive and reversible. Remarkably, treatment with cysteine resulted in reversal or prevention of Na+/K+-ATPase inhibition. It is very likely that the described effects may contribute to the overall cytotoxic profile of these gold complexes. (C) ...2014 Elsevier Inc. All rights reserved.
Keywords:Sodium pump / Gold(III) complexes / Human blood cells / Inhibition / Cytotoxicity
Source:Journal of Inorganic Biochemistry, 2014, 140, 228-235
- Studies of enzyme interactions with toxic and pharmacologically active molecules (RS-172023)
- AIRC [IG-12085], Beneficentia Stiftung Vaduz