Light controllable TiO2-Ru nanocomposite system encapsulated in small unilamellar vesicles for anti-cancer photodynamic therapy
2017
Преузимање 🢃
Аутори
Matijević, MilicaNešić, Maja D.
Popović, Iva A.
Stepić, Milutin S.
Radoičić, Marija B.
Šaponjić, Zoran
Petković, Marijana
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Approximately 13 million of new cancer cases are diagnosed every year, and the mortality rate is projected to rise, with an estimation of 13.1 million deaths in 2030 [1]. The conventional cancer treatments such as chemotherapy, radiotherapy and surgery suffer from serious drawbacks that hamper patients’ healing and recovery: radiation therapy is limited by the cumulative radiation dose,chemotherapy is frequently associated with systemic side-effects, while rather high recurrence rate is associated with surgical resection of tumors. Photodynamic therapy, which implies combined use of a photosensitising medicament and low-intensity light to cause selective damage to the target tissue, is an alternative tumor-ablative, function-sparing and costeffective oncologic approach [2]. Usually, medicaments are delivered to diseased tissues by different types of nanoparticle (NP) carriers. The light-induced medicament activation is based on the intrinsic optical properties of a medicament carrier a...nd a medicament itself. In most cases, a NP-based medicament carrier or a medicament itself acts as photosensitizers [PSs], by triggering the free radical reactions in cells leading eventually to the cell death. The most PSs are characterized by high lipophilicity, and due to that, different encapsulation strategies have been explored to protect the hydrophobic PS from the aqueous environment. We used TiO2 NPs for medicament carriers because of their availability, non-toxicity, stability and possibility of surface modification. On the other hand, Ru(II)(dcbpy)2Cl2 complex, that belongs to the second generation of metallodrugs, can be easily attached to the TiO2 surface via carboxyl groups [3], while central metal ion remains free for the interaction with biomolecules. Additionally, this system has genotoxic effect on melanoma cells [4]. The aim of the present study was to modify the nanocomposite system (NCS) based on the Ru complex and TiO2 NPs in order to increase the cellular uptake and its efficiency against tumor cell lines. We have stabilized a colloidal dispersion of TiO2 NPs on pH 7 by surface modification, whereas NCS was formed as described in our previous work [4]. NCS was encapsulated in the small unilamellar vesicles made mainly of phosphatidylcholine and phosphatidylethanol-amine [5]. Binding of Ru-complex to the surface of the TiO2 NPs was confirmed by Fourier transform infrared spectroscopy. The influence of light of various wavelengths and intensity on the complex release kinetics from encapsulated and non-encapsulated NCS was investigated, and both these systems had shown exceptional light-controllable complex-release ability.
Извор:
PHOTONICA2017 : 6th International School and Conference on Photonics and COST actions: MP1406 and MP1402 : Program and the book of abstracts, 2017, 111-111Издавач:
- Belgrade : Institute of Physics Belgrade
Напомена:
- VI International School and Conference on Photonics and COST actions: MP1406 and MP1402 : PHOTONICA2017 : August 23 - September 1, 2017; Belgrade.
Колекције
Институција/група
VinčaTY - CONF AU - Matijević, Milica AU - Nešić, Maja D. AU - Popović, Iva A. AU - Stepić, Milutin S. AU - Radoičić, Marija B. AU - Šaponjić, Zoran AU - Petković, Marijana PY - 2017 UR - https://vinar.vin.bg.ac.rs/handle/123456789/13348 AB - Approximately 13 million of new cancer cases are diagnosed every year, and the mortality rate is projected to rise, with an estimation of 13.1 million deaths in 2030 [1]. The conventional cancer treatments such as chemotherapy, radiotherapy and surgery suffer from serious drawbacks that hamper patients’ healing and recovery: radiation therapy is limited by the cumulative radiation dose,chemotherapy is frequently associated with systemic side-effects, while rather high recurrence rate is associated with surgical resection of tumors. Photodynamic therapy, which implies combined use of a photosensitising medicament and low-intensity light to cause selective damage to the target tissue, is an alternative tumor-ablative, function-sparing and costeffective oncologic approach [2]. Usually, medicaments are delivered to diseased tissues by different types of nanoparticle (NP) carriers. The light-induced medicament activation is based on the intrinsic optical properties of a medicament carrier and a medicament itself. In most cases, a NP-based medicament carrier or a medicament itself acts as photosensitizers [PSs], by triggering the free radical reactions in cells leading eventually to the cell death. The most PSs are characterized by high lipophilicity, and due to that, different encapsulation strategies have been explored to protect the hydrophobic PS from the aqueous environment. We used TiO2 NPs for medicament carriers because of their availability, non-toxicity, stability and possibility of surface modification. On the other hand, Ru(II)(dcbpy)2Cl2 complex, that belongs to the second generation of metallodrugs, can be easily attached to the TiO2 surface via carboxyl groups [3], while central metal ion remains free for the interaction with biomolecules. Additionally, this system has genotoxic effect on melanoma cells [4]. The aim of the present study was to modify the nanocomposite system (NCS) based on the Ru complex and TiO2 NPs in order to increase the cellular uptake and its efficiency against tumor cell lines. We have stabilized a colloidal dispersion of TiO2 NPs on pH 7 by surface modification, whereas NCS was formed as described in our previous work [4]. NCS was encapsulated in the small unilamellar vesicles made mainly of phosphatidylcholine and phosphatidylethanol-amine [5]. Binding of Ru-complex to the surface of the TiO2 NPs was confirmed by Fourier transform infrared spectroscopy. The influence of light of various wavelengths and intensity on the complex release kinetics from encapsulated and non-encapsulated NCS was investigated, and both these systems had shown exceptional light-controllable complex-release ability. PB - Belgrade : Institute of Physics Belgrade C3 - PHOTONICA2017 : 6th International School and Conference on Photonics and COST actions: MP1406 and MP1402 : Program and the book of abstracts T1 - Light controllable TiO2-Ru nanocomposite system encapsulated in small unilamellar vesicles for anti-cancer photodynamic therapy SP - 111 EP - 111 UR - https://hdl.handle.net/21.15107/rcub_vinar_13348 ER -
@conference{ author = "Matijević, Milica and Nešić, Maja D. and Popović, Iva A. and Stepić, Milutin S. and Radoičić, Marija B. and Šaponjić, Zoran and Petković, Marijana", year = "2017", abstract = "Approximately 13 million of new cancer cases are diagnosed every year, and the mortality rate is projected to rise, with an estimation of 13.1 million deaths in 2030 [1]. The conventional cancer treatments such as chemotherapy, radiotherapy and surgery suffer from serious drawbacks that hamper patients’ healing and recovery: radiation therapy is limited by the cumulative radiation dose,chemotherapy is frequently associated with systemic side-effects, while rather high recurrence rate is associated with surgical resection of tumors. Photodynamic therapy, which implies combined use of a photosensitising medicament and low-intensity light to cause selective damage to the target tissue, is an alternative tumor-ablative, function-sparing and costeffective oncologic approach [2]. Usually, medicaments are delivered to diseased tissues by different types of nanoparticle (NP) carriers. The light-induced medicament activation is based on the intrinsic optical properties of a medicament carrier and a medicament itself. In most cases, a NP-based medicament carrier or a medicament itself acts as photosensitizers [PSs], by triggering the free radical reactions in cells leading eventually to the cell death. The most PSs are characterized by high lipophilicity, and due to that, different encapsulation strategies have been explored to protect the hydrophobic PS from the aqueous environment. We used TiO2 NPs for medicament carriers because of their availability, non-toxicity, stability and possibility of surface modification. On the other hand, Ru(II)(dcbpy)2Cl2 complex, that belongs to the second generation of metallodrugs, can be easily attached to the TiO2 surface via carboxyl groups [3], while central metal ion remains free for the interaction with biomolecules. Additionally, this system has genotoxic effect on melanoma cells [4]. The aim of the present study was to modify the nanocomposite system (NCS) based on the Ru complex and TiO2 NPs in order to increase the cellular uptake and its efficiency against tumor cell lines. We have stabilized a colloidal dispersion of TiO2 NPs on pH 7 by surface modification, whereas NCS was formed as described in our previous work [4]. NCS was encapsulated in the small unilamellar vesicles made mainly of phosphatidylcholine and phosphatidylethanol-amine [5]. Binding of Ru-complex to the surface of the TiO2 NPs was confirmed by Fourier transform infrared spectroscopy. The influence of light of various wavelengths and intensity on the complex release kinetics from encapsulated and non-encapsulated NCS was investigated, and both these systems had shown exceptional light-controllable complex-release ability.", publisher = "Belgrade : Institute of Physics Belgrade", journal = "PHOTONICA2017 : 6th International School and Conference on Photonics and COST actions: MP1406 and MP1402 : Program and the book of abstracts", title = "Light controllable TiO2-Ru nanocomposite system encapsulated in small unilamellar vesicles for anti-cancer photodynamic therapy", pages = "111-111", url = "https://hdl.handle.net/21.15107/rcub_vinar_13348" }
Matijević, M., Nešić, M. D., Popović, I. A., Stepić, M. S., Radoičić, M. B., Šaponjić, Z.,& Petković, M.. (2017). Light controllable TiO2-Ru nanocomposite system encapsulated in small unilamellar vesicles for anti-cancer photodynamic therapy. in PHOTONICA2017 : 6th International School and Conference on Photonics and COST actions: MP1406 and MP1402 : Program and the book of abstracts Belgrade : Institute of Physics Belgrade., 111-111. https://hdl.handle.net/21.15107/rcub_vinar_13348
Matijević M, Nešić MD, Popović IA, Stepić MS, Radoičić MB, Šaponjić Z, Petković M. Light controllable TiO2-Ru nanocomposite system encapsulated in small unilamellar vesicles for anti-cancer photodynamic therapy. in PHOTONICA2017 : 6th International School and Conference on Photonics and COST actions: MP1406 and MP1402 : Program and the book of abstracts. 2017;:111-111. https://hdl.handle.net/21.15107/rcub_vinar_13348 .
Matijević, Milica, Nešić, Maja D., Popović, Iva A., Stepić, Milutin S., Radoičić, Marija B., Šaponjić, Zoran, Petković, Marijana, "Light controllable TiO2-Ru nanocomposite system encapsulated in small unilamellar vesicles for anti-cancer photodynamic therapy" in PHOTONICA2017 : 6th International School and Conference on Photonics and COST actions: MP1406 and MP1402 : Program and the book of abstracts (2017):111-111, https://hdl.handle.net/21.15107/rcub_vinar_13348 .