In vitro toxicity evaluation of potential contrast agents for micro-computed tomography: effect on cell membrane
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2022
Authors
Domijan, Ana-MarijaGajski, Goran
Gerić, M.
Savić, Nada
Parac-Vogt, Tatjana
Krstić, Danijela
Čolović, Mirjana
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In this study, prooxidative properties of three Wells-Dawson polyoxometalates (POMs) previously reported as promising contrast-enhancing staining agents for micro-computed tomography (microCT): parent Wells-Dawson POM α2-K6P2W18O62.14H2O (parent WD), monolacunary Wells-Dawson POM α2-K10P2W17O61.20H2O (lacunary WD), and 1:2 hafnium(IV)-substituted Wells-Dawson POM K16[Hf(α2-P2W17O61)2]·19H2O (Hf-WD 1:2), were evaluated in vitro. In this aim, malondialdehyde (MDA) level, as an indicator of prooxidant action and lipid peroxidation, was determined in human plasma after 24 h treatment with the POMs in the concentration range 1-100 μM at 37 °C. The results demonstrated that the studied POMs did not induce an increase in plasma lipid peroxidation level compared to the corresponding control. Thus, it could be concluded that the investigated POM-based contrast agent candidates for microCT are not sufficiently potent prooxidants capable to cause membrane damage and consequent toxic occurrences.
Source:
PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings, 2022, 1, 283-286Publisher:
- Belgrade : Society of Physical Chemists of Serbia
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200017 (University of Belgrade, Institute of Nuclear Sciences 'Vinča', Belgrade-Vinča) (RS-MESTD-inst-2020-200017)
- POMCACT - In Vitro and in Vivo Evaluation of Novel Polyoxometalate Based Contrast Agents for Micro Computed Tomography (RS-ScienceFundRS-Dijaspora-6526393)
- Bilateral project Serbia-Croatia [No. 337-00-205/2019-09/19]
- Erasmus+ KA103 European Mobility Program
Note:
- 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : September 26-30, Belgrade.
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VinčaTY - CONF AU - Domijan, Ana-Marija AU - Gajski, Goran AU - Gerić, M. AU - Savić, Nada AU - Parac-Vogt, Tatjana AU - Krstić, Danijela AU - Čolović, Mirjana PY - 2022 UR - https://vinar.vin.bg.ac.rs/handle/123456789/12926 AB - In this study, prooxidative properties of three Wells-Dawson polyoxometalates (POMs) previously reported as promising contrast-enhancing staining agents for micro-computed tomography (microCT): parent Wells-Dawson POM α2-K6P2W18O62.14H2O (parent WD), monolacunary Wells-Dawson POM α2-K10P2W17O61.20H2O (lacunary WD), and 1:2 hafnium(IV)-substituted Wells-Dawson POM K16[Hf(α2-P2W17O61)2]·19H2O (Hf-WD 1:2), were evaluated in vitro. In this aim, malondialdehyde (MDA) level, as an indicator of prooxidant action and lipid peroxidation, was determined in human plasma after 24 h treatment with the POMs in the concentration range 1-100 μM at 37 °C. The results demonstrated that the studied POMs did not induce an increase in plasma lipid peroxidation level compared to the corresponding control. Thus, it could be concluded that the investigated POM-based contrast agent candidates for microCT are not sufficiently potent prooxidants capable to cause membrane damage and consequent toxic occurrences. PB - Belgrade : Society of Physical Chemists of Serbia C3 - PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings T1 - In vitro toxicity evaluation of potential contrast agents for micro-computed tomography: effect on cell membrane VL - 1 SP - 283 EP - 286 UR - https://hdl.handle.net/21.15107/rcub_vinar_12926 ER -
@conference{ author = "Domijan, Ana-Marija and Gajski, Goran and Gerić, M. and Savić, Nada and Parac-Vogt, Tatjana and Krstić, Danijela and Čolović, Mirjana", year = "2022", abstract = "In this study, prooxidative properties of three Wells-Dawson polyoxometalates (POMs) previously reported as promising contrast-enhancing staining agents for micro-computed tomography (microCT): parent Wells-Dawson POM α2-K6P2W18O62.14H2O (parent WD), monolacunary Wells-Dawson POM α2-K10P2W17O61.20H2O (lacunary WD), and 1:2 hafnium(IV)-substituted Wells-Dawson POM K16[Hf(α2-P2W17O61)2]·19H2O (Hf-WD 1:2), were evaluated in vitro. In this aim, malondialdehyde (MDA) level, as an indicator of prooxidant action and lipid peroxidation, was determined in human plasma after 24 h treatment with the POMs in the concentration range 1-100 μM at 37 °C. The results demonstrated that the studied POMs did not induce an increase in plasma lipid peroxidation level compared to the corresponding control. Thus, it could be concluded that the investigated POM-based contrast agent candidates for microCT are not sufficiently potent prooxidants capable to cause membrane damage and consequent toxic occurrences.", publisher = "Belgrade : Society of Physical Chemists of Serbia", journal = "PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings", title = "In vitro toxicity evaluation of potential contrast agents for micro-computed tomography: effect on cell membrane", volume = "1", pages = "283-286", url = "https://hdl.handle.net/21.15107/rcub_vinar_12926" }
Domijan, A., Gajski, G., Gerić, M., Savić, N., Parac-Vogt, T., Krstić, D.,& Čolović, M.. (2022). In vitro toxicity evaluation of potential contrast agents for micro-computed tomography: effect on cell membrane. in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings Belgrade : Society of Physical Chemists of Serbia., 1, 283-286. https://hdl.handle.net/21.15107/rcub_vinar_12926
Domijan A, Gajski G, Gerić M, Savić N, Parac-Vogt T, Krstić D, Čolović M. In vitro toxicity evaluation of potential contrast agents for micro-computed tomography: effect on cell membrane. in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings. 2022;1:283-286. https://hdl.handle.net/21.15107/rcub_vinar_12926 .
Domijan, Ana-Marija, Gajski, Goran, Gerić, M., Savić, Nada, Parac-Vogt, Tatjana, Krstić, Danijela, Čolović, Mirjana, "In vitro toxicity evaluation of potential contrast agents for micro-computed tomography: effect on cell membrane" in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings, 1 (2022):283-286, https://hdl.handle.net/21.15107/rcub_vinar_12926 .