Melatonin mediated antidepressant-like effect in the hippocampus of chronic stress-induced depression rats: Regulating vesicular monoamine transporter 2 and monoamine oxidase A levels
Samo za registrovane korisnike
2016
Autori
Stefanović, BojanaSpasojević, Nataša
Jovanović, Predrag
Jasnić, Nebojša
Đorđević, Jelena D.
Dronjak, Slađana
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The hippocampus is sensitive to stress which activates norepinephrine terminals deriving from the locus coeruleus. Melatonin exerts positive effects on the hippocampal neurogenic process and on depressive-like behaviour. Thus, in the present study, an examination was made of the effect of chronic melatonin treatment on norepinephrine content, synthesis, uptake, vesicular transport and degradation in the hippocampus of rats exposed to CUMS. This entailed quantifying the norephinephrine, mRNA and protein levels of DBH, NET, VMAT 2, MAO-A and COMT. The results show that CUMS evoked prolonged immobility. Melatonin treatment decreased immobility in comparison with the placebo group, reflecting an antidepressant-like effect. Compared with the placebo group, a dramatic decrease in norepinephrine content, decreased VMAT2 mRNA and protein and increased MAO-A protein levels in the hippocampus of the CUMS rats were observed. However, no significant differences in the levels of DBH, NET, COMT mRNA... and protein and MAO-A mRNA levels between the placebo and the stressed groups were found. The results showed the restorative effects of melatonin on the stress-induced decline in the norepinephrine content of the hippocampus. It was observed that melatonin treatment in the CUMS rats prevented the stress-induced decrease in VMAT2 mRNA and protein levels, whereas it reduced the increase of the mRNA of COMT and protein levels of MAO-A. Chronic treatment with melatonin failed to alter the gene expression of DBH or NET in the hippocampus of the CUMS rats. Additionally, the results show that melatonin enhances VMAT2 expression and norepinephrine storage, whilst it reduces norepinephrine degrading enzymes. (C) 2016 Elsevier B.V. and ECNP. All rights reserved.
Ključne reči:
Melatonin / Norepinephrine / Hippocampus / Depression / Enzymes / TransportersIzvor:
European Neuropsychopharmacology, 2016, 26, 10, 1629-1637Finansiranje / projekti:
- Molekularni mehanizmi patofizioloških promena u ćelijama centralnog nervnog sistema i perifernog tkiva kod sisara (RS-173044)
- Efekat metaboličkih i nemetaboličkih stresora na ekspresiju i delovanje neuroendokrinih regulatora energetske homeostaze (RS-173023)
DOI: 10.1016/j.euroneuro.2016.07.005
ISSN: 0924-977X; 1873-7862
PubMed: 27499503
WoS: 000384869300008
Scopus: 2-s2.0-84991065457
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Stefanović, Bojana AU - Spasojević, Nataša AU - Jovanović, Predrag AU - Jasnić, Nebojša AU - Đorđević, Jelena D. AU - Dronjak, Slađana PY - 2016 UR - https://vinar.vin.bg.ac.rs/handle/123456789/1265 AB - The hippocampus is sensitive to stress which activates norepinephrine terminals deriving from the locus coeruleus. Melatonin exerts positive effects on the hippocampal neurogenic process and on depressive-like behaviour. Thus, in the present study, an examination was made of the effect of chronic melatonin treatment on norepinephrine content, synthesis, uptake, vesicular transport and degradation in the hippocampus of rats exposed to CUMS. This entailed quantifying the norephinephrine, mRNA and protein levels of DBH, NET, VMAT 2, MAO-A and COMT. The results show that CUMS evoked prolonged immobility. Melatonin treatment decreased immobility in comparison with the placebo group, reflecting an antidepressant-like effect. Compared with the placebo group, a dramatic decrease in norepinephrine content, decreased VMAT2 mRNA and protein and increased MAO-A protein levels in the hippocampus of the CUMS rats were observed. However, no significant differences in the levels of DBH, NET, COMT mRNA and protein and MAO-A mRNA levels between the placebo and the stressed groups were found. The results showed the restorative effects of melatonin on the stress-induced decline in the norepinephrine content of the hippocampus. It was observed that melatonin treatment in the CUMS rats prevented the stress-induced decrease in VMAT2 mRNA and protein levels, whereas it reduced the increase of the mRNA of COMT and protein levels of MAO-A. Chronic treatment with melatonin failed to alter the gene expression of DBH or NET in the hippocampus of the CUMS rats. Additionally, the results show that melatonin enhances VMAT2 expression and norepinephrine storage, whilst it reduces norepinephrine degrading enzymes. (C) 2016 Elsevier B.V. and ECNP. All rights reserved. T2 - European Neuropsychopharmacology T1 - Melatonin mediated antidepressant-like effect in the hippocampus of chronic stress-induced depression rats: Regulating vesicular monoamine transporter 2 and monoamine oxidase A levels VL - 26 IS - 10 SP - 1629 EP - 1637 DO - 10.1016/j.euroneuro.2016.07.005 ER -
@article{ author = "Stefanović, Bojana and Spasojević, Nataša and Jovanović, Predrag and Jasnić, Nebojša and Đorđević, Jelena D. and Dronjak, Slađana", year = "2016", abstract = "The hippocampus is sensitive to stress which activates norepinephrine terminals deriving from the locus coeruleus. Melatonin exerts positive effects on the hippocampal neurogenic process and on depressive-like behaviour. Thus, in the present study, an examination was made of the effect of chronic melatonin treatment on norepinephrine content, synthesis, uptake, vesicular transport and degradation in the hippocampus of rats exposed to CUMS. This entailed quantifying the norephinephrine, mRNA and protein levels of DBH, NET, VMAT 2, MAO-A and COMT. The results show that CUMS evoked prolonged immobility. Melatonin treatment decreased immobility in comparison with the placebo group, reflecting an antidepressant-like effect. Compared with the placebo group, a dramatic decrease in norepinephrine content, decreased VMAT2 mRNA and protein and increased MAO-A protein levels in the hippocampus of the CUMS rats were observed. However, no significant differences in the levels of DBH, NET, COMT mRNA and protein and MAO-A mRNA levels between the placebo and the stressed groups were found. The results showed the restorative effects of melatonin on the stress-induced decline in the norepinephrine content of the hippocampus. It was observed that melatonin treatment in the CUMS rats prevented the stress-induced decrease in VMAT2 mRNA and protein levels, whereas it reduced the increase of the mRNA of COMT and protein levels of MAO-A. Chronic treatment with melatonin failed to alter the gene expression of DBH or NET in the hippocampus of the CUMS rats. Additionally, the results show that melatonin enhances VMAT2 expression and norepinephrine storage, whilst it reduces norepinephrine degrading enzymes. (C) 2016 Elsevier B.V. and ECNP. All rights reserved.", journal = "European Neuropsychopharmacology", title = "Melatonin mediated antidepressant-like effect in the hippocampus of chronic stress-induced depression rats: Regulating vesicular monoamine transporter 2 and monoamine oxidase A levels", volume = "26", number = "10", pages = "1629-1637", doi = "10.1016/j.euroneuro.2016.07.005" }
Stefanović, B., Spasojević, N., Jovanović, P., Jasnić, N., Đorđević, J. D.,& Dronjak, S.. (2016). Melatonin mediated antidepressant-like effect in the hippocampus of chronic stress-induced depression rats: Regulating vesicular monoamine transporter 2 and monoamine oxidase A levels. in European Neuropsychopharmacology, 26(10), 1629-1637. https://doi.org/10.1016/j.euroneuro.2016.07.005
Stefanović B, Spasojević N, Jovanović P, Jasnić N, Đorđević JD, Dronjak S. Melatonin mediated antidepressant-like effect in the hippocampus of chronic stress-induced depression rats: Regulating vesicular monoamine transporter 2 and monoamine oxidase A levels. in European Neuropsychopharmacology. 2016;26(10):1629-1637. doi:10.1016/j.euroneuro.2016.07.005 .
Stefanović, Bojana, Spasojević, Nataša, Jovanović, Predrag, Jasnić, Nebojša, Đorđević, Jelena D., Dronjak, Slađana, "Melatonin mediated antidepressant-like effect in the hippocampus of chronic stress-induced depression rats: Regulating vesicular monoamine transporter 2 and monoamine oxidase A levels" in European Neuropsychopharmacology, 26, no. 10 (2016):1629-1637, https://doi.org/10.1016/j.euroneuro.2016.07.005 . .