Insight in miRNome of severe multiple sclerosis: Pilot study of distinctive relapse-onset MS phenotypes
Autori
Stanković, Aleksandra![](/themes/MirageVinar/images/orcid.png)
Jovanović, Ivan G.
![](/themes/MirageVinar/images/orcid.png)
Dinčić, E.
Vojinović, S.
Stojković, Ljiljana S.
![](/themes/MirageVinar/images/orcid.png)
Đorđević, Ana
![](/themes/MirageVinar/images/orcid.png)
Kuveljić, Jovana
![](/themes/MirageVinar/images/orcid.png)
Živković, Maja
![](/themes/MirageVinar/images/orcid.png)
Konferencijski prilog (Objavljena verzija)
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Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Molecular background and biomarkers of highly heterogenous and hardly predictable disease progression among relapse-onset MS patients are of high research interest. In the current pilot study, we aimed to employ next-generation sequencing to investigate the expression of whole small non-coding microRNAs (miRNome) in two groups of MS patients with highly distinctive progression phenotype: one with fast progressing, severely disabling course vs. mild course of MS, longitudinally followed 10 years. Peripheral blood mononuclear cells (PBMC) miRNome data was obtained from mild phenotype MS (n=4 patients) and progressive phenotype MS (n=5 patients), using TakaraBio SMARTer smRNA-Seq Kit on iSeq100 (Illumina). Pre-processing of raw sequencing data, quality control and miRNA differential expression analysis was performed using sRNAtoolbox pipeline. Functional interpretation of differentially expressed miRNA target genes was done in DIANA-miRPathv3.0. Tarbase v8.0 served as a resource of miRNA:...gene interactions. Achieved read depth was approximately 1 million raw reads/sample, allowing detection of up to 92 mature miRNAs after genome alignment and miRbase v22 annotation. Differential expression analysis identified the significant upregulation of hsa-miR-23c (log2FC=4.29, Padj= 0.03) in progressive phenotype. Top significantly enriched KEGG pathways in hsa-miR-23c targets suggested regulation of molecular pathways involved in autoimmunity (antigen presentation, Epstein-Barr virus infection) and cancer. In conclusion, this pilot study indicates phenotype-related differences in expression of miRNAs, molecules with high regulatory and biomarker properties. Although detected in PBMC, has-miR-23c is highly expressed in the brain and target MS relevant genes such as, HLA (A, B, C), transferrin receptor, Nrf2, recently proposed to play important role in neurodegeneration.
Izvor:
17th World Congress on Controversies in Neurology (CONy) : e-posters, 2023, 427-427URI
https://web.archive.org/web/20240131090652/https://cony2023.comtecmed.com/e-posters/https://vinar.vin.bg.ac.rs/handle/123456789/12641
Kolekcije
Institucija/grupa
VinčaTY - CONF AU - Stanković, Aleksandra AU - Jovanović, Ivan G. AU - Dinčić, E. AU - Vojinović, S. AU - Stojković, Ljiljana S. AU - Đorđević, Ana AU - Kuveljić, Jovana AU - Živković, Maja PY - 2023 UR - https://web.archive.org/web/20240131090652/https://cony2023.comtecmed.com/e-posters/ UR - https://vinar.vin.bg.ac.rs/handle/123456789/12641 AB - Molecular background and biomarkers of highly heterogenous and hardly predictable disease progression among relapse-onset MS patients are of high research interest. In the current pilot study, we aimed to employ next-generation sequencing to investigate the expression of whole small non-coding microRNAs (miRNome) in two groups of MS patients with highly distinctive progression phenotype: one with fast progressing, severely disabling course vs. mild course of MS, longitudinally followed 10 years. Peripheral blood mononuclear cells (PBMC) miRNome data was obtained from mild phenotype MS (n=4 patients) and progressive phenotype MS (n=5 patients), using TakaraBio SMARTer smRNA-Seq Kit on iSeq100 (Illumina). Pre-processing of raw sequencing data, quality control and miRNA differential expression analysis was performed using sRNAtoolbox pipeline. Functional interpretation of differentially expressed miRNA target genes was done in DIANA-miRPathv3.0. Tarbase v8.0 served as a resource of miRNA:gene interactions. Achieved read depth was approximately 1 million raw reads/sample, allowing detection of up to 92 mature miRNAs after genome alignment and miRbase v22 annotation. Differential expression analysis identified the significant upregulation of hsa-miR-23c (log2FC=4.29, Padj= 0.03) in progressive phenotype. Top significantly enriched KEGG pathways in hsa-miR-23c targets suggested regulation of molecular pathways involved in autoimmunity (antigen presentation, Epstein-Barr virus infection) and cancer. In conclusion, this pilot study indicates phenotype-related differences in expression of miRNAs, molecules with high regulatory and biomarker properties. Although detected in PBMC, has-miR-23c is highly expressed in the brain and target MS relevant genes such as, HLA (A, B, C), transferrin receptor, Nrf2, recently proposed to play important role in neurodegeneration. C3 - 17th World Congress on Controversies in Neurology (CONy) : e-posters T1 - Insight in miRNome of severe multiple sclerosis: Pilot study of distinctive relapse-onset MS phenotypes SP - 427 EP - 427 UR - https://hdl.handle.net/21.15107/rcub_vinar_12641 ER -
@conference{ author = "Stanković, Aleksandra and Jovanović, Ivan G. and Dinčić, E. and Vojinović, S. and Stojković, Ljiljana S. and Đorđević, Ana and Kuveljić, Jovana and Živković, Maja", year = "2023", abstract = "Molecular background and biomarkers of highly heterogenous and hardly predictable disease progression among relapse-onset MS patients are of high research interest. In the current pilot study, we aimed to employ next-generation sequencing to investigate the expression of whole small non-coding microRNAs (miRNome) in two groups of MS patients with highly distinctive progression phenotype: one with fast progressing, severely disabling course vs. mild course of MS, longitudinally followed 10 years. Peripheral blood mononuclear cells (PBMC) miRNome data was obtained from mild phenotype MS (n=4 patients) and progressive phenotype MS (n=5 patients), using TakaraBio SMARTer smRNA-Seq Kit on iSeq100 (Illumina). Pre-processing of raw sequencing data, quality control and miRNA differential expression analysis was performed using sRNAtoolbox pipeline. Functional interpretation of differentially expressed miRNA target genes was done in DIANA-miRPathv3.0. Tarbase v8.0 served as a resource of miRNA:gene interactions. Achieved read depth was approximately 1 million raw reads/sample, allowing detection of up to 92 mature miRNAs after genome alignment and miRbase v22 annotation. Differential expression analysis identified the significant upregulation of hsa-miR-23c (log2FC=4.29, Padj= 0.03) in progressive phenotype. Top significantly enriched KEGG pathways in hsa-miR-23c targets suggested regulation of molecular pathways involved in autoimmunity (antigen presentation, Epstein-Barr virus infection) and cancer. In conclusion, this pilot study indicates phenotype-related differences in expression of miRNAs, molecules with high regulatory and biomarker properties. Although detected in PBMC, has-miR-23c is highly expressed in the brain and target MS relevant genes such as, HLA (A, B, C), transferrin receptor, Nrf2, recently proposed to play important role in neurodegeneration.", journal = "17th World Congress on Controversies in Neurology (CONy) : e-posters", title = "Insight in miRNome of severe multiple sclerosis: Pilot study of distinctive relapse-onset MS phenotypes", pages = "427-427", url = "https://hdl.handle.net/21.15107/rcub_vinar_12641" }
Stanković, A., Jovanović, I. G., Dinčić, E., Vojinović, S., Stojković, L. S., Đorđević, A., Kuveljić, J.,& Živković, M.. (2023). Insight in miRNome of severe multiple sclerosis: Pilot study of distinctive relapse-onset MS phenotypes. in 17th World Congress on Controversies in Neurology (CONy) : e-posters, 427-427. https://hdl.handle.net/21.15107/rcub_vinar_12641
Stanković A, Jovanović IG, Dinčić E, Vojinović S, Stojković LS, Đorđević A, Kuveljić J, Živković M. Insight in miRNome of severe multiple sclerosis: Pilot study of distinctive relapse-onset MS phenotypes. in 17th World Congress on Controversies in Neurology (CONy) : e-posters. 2023;:427-427. https://hdl.handle.net/21.15107/rcub_vinar_12641 .
Stanković, Aleksandra, Jovanović, Ivan G., Dinčić, E., Vojinović, S., Stojković, Ljiljana S., Đorđević, Ana, Kuveljić, Jovana, Živković, Maja, "Insight in miRNome of severe multiple sclerosis: Pilot study of distinctive relapse-onset MS phenotypes" in 17th World Congress on Controversies in Neurology (CONy) : e-posters (2023):427-427, https://hdl.handle.net/21.15107/rcub_vinar_12641 .