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dc.creatorPajović, Jelena D.
dc.creatorDojčilović, Radovan
dc.creatorKaščáková, Slávka
dc.creatorRéfrégiers, Matthieu
dc.creatorBožanić, Dušan K.
dc.creatorĐoković, Vladimir
dc.date.accessioned2023-05-29T10:53:56Z
dc.date.available2023-05-29T10:53:56Z
dc.date.issued2023
dc.identifier.issn0927-7765
dc.identifier.urihttps://vinar.vin.bg.ac.rs/handle/123456789/11022
dc.description.abstractGold nanoparticles were functionalized by amino acid tryptophan and vitamin riboflavin - a resonance energy transfer (RET) pair of biomolecules. The presence of the gold nanoparticles resulted in 65% increase in RET efficiency. Because of enhanced RET efficiency, the photobleaching dynamics of the fluorescent molecules at the surface of the nanoparticles is different from that of molecules in solution. The observed effect was used for detection of the functionalized nanoparticles within biological material rich with autofluorescent species. Synchrotron radiation deep-ultraviolet fluorescence microscopy is used to study the photobleaching dynamics of the fluorescence centers within human hepatocellular carcinoma Huh7.5.1 cells incubated with the nanoparticles. The fluorescent centers were classified according to their photobleaching dynamics, which enabled the discrimination of the cell areas where the accumulation of the nanoparticles takes place, even though the particles were smaller than the spatial resolution of the images.en
dc.languageen
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200162/RS//
dc.relationSynchrotron Soleil (France) - [Project No. 20131218 ]
dc.rightsrestrictedAccess
dc.sourceColloids and Surfaces B: Biointerfaces
dc.subjectFluorescence imagingen
dc.subjectGold nanoparticlesen
dc.subjectPhotobleachingen
dc.subjectResonance energy transferen
dc.subjectRiboflavinen
dc.subjectTryptophanen
dc.titleEnhanced resonance energy transfer in gold nanoparticles bifunctionalized by tryptophan and riboflavin and its application in fluorescence bioimagingen
dc.typearticleen
dc.rights.licenseARR
dc.citation.volume227
dc.citation.spage113340
dc.identifier.doi10.1016/j.colsurfb.2023.113340
dc.type.versionpublishedVersion
dc.identifier.scopus2-s2.0-85159420647


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