Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin
Authors
Ponjavić, Marijana
Malagurski, Ivana
Lazić, Jelena

Jeremić, Sanja

Pavlović, Vladimir B.

Prlainović, Nevena
Maksimović, Vesna

Ćosović, Vladan

Atanase, Leonard Ionut

Freitas, Filomena

Matos, Mariana

Nikodinović-Runić, Jasmina

Article (Published version)
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The quest for sustainable biomaterials with excellent biocompatibility and tailorableproperties has put polyhydroxyalkanoates (PHAs) into the research spotlight. However, high productioncosts and the lack of bioactivity limit their market penetration. To address this, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was combined with a bacterial pigment with stronganticancer activity, prodigiosin (PG), to obtain functionally enhanced PHBV-based biomaterials. Thesamples were produced in the form of films 115.6–118.8 m in thickness using the solvent castingmethod. The effects of PG incorporation on the physical properties (morphology, biopolymer crystallinityand thermal stability) and functionality of the obtained biomaterials were investigated. PGhas acted as a nucleating agent, in turn affecting the degree of crystallinity, thermal stability andmorphology of the films. All samples with PG had a more organized internal structure and highermelting and degradation temperatures. The ca...lculated degree of crystallinity of the PHBV copolymerwas 53%, while the PG1, PG3 and PG3 films had values of 64.0%, 63.9% and 69.2%, respectively.Cytotoxicity studies have shown the excellent anticancer activity of films against HCT116 (coloncancer) cells, thus advancing PHBV biomedical application potential.
Keywords:
prodigiosin / polyhydroxyalkanoate / PHBV / anticancer activity / film / drug delivery / biopolymer crystallinity / biocompatibilitySource:
International Journal of Molecular Sciences, 2023, 24, 3, 1906-Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200042 (University of Belgrade, Institute of Molecular Genetics and Genetic Engineering) (RS-200042)
- BioECOLogics - Value-added biologics through eco-sustainable routes (RS-7730810)
- Associate Laboratory Institute for Health and Bioeconomy—i4HB [project LA/P/0140/202019]
- FCT—Fundação para a Ciência e a Tecnologia and Research Unit on Applied Molecular Biosciences - UCIBIO - [UIDP/04378/2020 and UIDB/04378/2020]
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VinčaTY - JOUR AU - Ponjavić, Marijana AU - Malagurski, Ivana AU - Lazić, Jelena AU - Jeremić, Sanja AU - Pavlović, Vladimir B. AU - Prlainović, Nevena AU - Maksimović, Vesna AU - Ćosović, Vladan AU - Atanase, Leonard Ionut AU - Freitas, Filomena AU - Matos, Mariana AU - Nikodinović-Runić, Jasmina PY - 2023 UR - https://vinar.vin.bg.ac.rs/handle/123456789/10660 AB - The quest for sustainable biomaterials with excellent biocompatibility and tailorableproperties has put polyhydroxyalkanoates (PHAs) into the research spotlight. However, high productioncosts and the lack of bioactivity limit their market penetration. To address this, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was combined with a bacterial pigment with stronganticancer activity, prodigiosin (PG), to obtain functionally enhanced PHBV-based biomaterials. Thesamples were produced in the form of films 115.6–118.8 m in thickness using the solvent castingmethod. The effects of PG incorporation on the physical properties (morphology, biopolymer crystallinityand thermal stability) and functionality of the obtained biomaterials were investigated. PGhas acted as a nucleating agent, in turn affecting the degree of crystallinity, thermal stability andmorphology of the films. All samples with PG had a more organized internal structure and highermelting and degradation temperatures. The calculated degree of crystallinity of the PHBV copolymerwas 53%, while the PG1, PG3 and PG3 films had values of 64.0%, 63.9% and 69.2%, respectively.Cytotoxicity studies have shown the excellent anticancer activity of films against HCT116 (coloncancer) cells, thus advancing PHBV biomedical application potential. T2 - International Journal of Molecular Sciences T1 - Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin VL - 24 IS - 3 SP - 1906 DO - 10.3390/ijms24031906 ER -
@article{ author = "Ponjavić, Marijana and Malagurski, Ivana and Lazić, Jelena and Jeremić, Sanja and Pavlović, Vladimir B. and Prlainović, Nevena and Maksimović, Vesna and Ćosović, Vladan and Atanase, Leonard Ionut and Freitas, Filomena and Matos, Mariana and Nikodinović-Runić, Jasmina", year = "2023", abstract = "The quest for sustainable biomaterials with excellent biocompatibility and tailorableproperties has put polyhydroxyalkanoates (PHAs) into the research spotlight. However, high productioncosts and the lack of bioactivity limit their market penetration. To address this, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was combined with a bacterial pigment with stronganticancer activity, prodigiosin (PG), to obtain functionally enhanced PHBV-based biomaterials. Thesamples were produced in the form of films 115.6–118.8 m in thickness using the solvent castingmethod. The effects of PG incorporation on the physical properties (morphology, biopolymer crystallinityand thermal stability) and functionality of the obtained biomaterials were investigated. PGhas acted as a nucleating agent, in turn affecting the degree of crystallinity, thermal stability andmorphology of the films. All samples with PG had a more organized internal structure and highermelting and degradation temperatures. The calculated degree of crystallinity of the PHBV copolymerwas 53%, while the PG1, PG3 and PG3 films had values of 64.0%, 63.9% and 69.2%, respectively.Cytotoxicity studies have shown the excellent anticancer activity of films against HCT116 (coloncancer) cells, thus advancing PHBV biomedical application potential.", journal = "International Journal of Molecular Sciences", title = "Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin", volume = "24", number = "3", pages = "1906", doi = "10.3390/ijms24031906" }
Ponjavić, M., Malagurski, I., Lazić, J., Jeremić, S., Pavlović, V. B., Prlainović, N., Maksimović, V., Ćosović, V., Atanase, L. I., Freitas, F., Matos, M.,& Nikodinović-Runić, J.. (2023). Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin. in International Journal of Molecular Sciences, 24(3), 1906. https://doi.org/10.3390/ijms24031906
Ponjavić M, Malagurski I, Lazić J, Jeremić S, Pavlović VB, Prlainović N, Maksimović V, Ćosović V, Atanase LI, Freitas F, Matos M, Nikodinović-Runić J. Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin. in International Journal of Molecular Sciences. 2023;24(3):1906. doi:10.3390/ijms24031906 .
Ponjavić, Marijana, Malagurski, Ivana, Lazić, Jelena, Jeremić, Sanja, Pavlović, Vladimir B., Prlainović, Nevena, Maksimović, Vesna, Ćosović, Vladan, Atanase, Leonard Ionut, Freitas, Filomena, Matos, Mariana, Nikodinović-Runić, Jasmina, "Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin" in International Journal of Molecular Sciences, 24, no. 3 (2023):1906, https://doi.org/10.3390/ijms24031906 . .